Cargando…

Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation

Context: Flaxseed has a characteristic fatty acids composition and unique phytonutrient profile that may have health-promoting properties. Objective: This study aimed to determine the effects of 10 weeks of supplementation with the flaxseed (28 g/day) on endothelial cells (EC) function, serum lipids...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanikowska, Dominika, Malińska, Agnieszka, Mickiewicz, Agnieszka, Zawada, Agnieszka, Rutkowski, Rafał, Pawlaczyk, Krzysztof, Sato, Maki, Bręborowicz, Andrzej, Witowski, Janusz, Korybalska, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315791/
https://www.ncbi.nlm.nih.gov/pubmed/35889835
http://dx.doi.org/10.3390/nu14142879
_version_ 1784754649524338688
author Kanikowska, Dominika
Malińska, Agnieszka
Mickiewicz, Agnieszka
Zawada, Agnieszka
Rutkowski, Rafał
Pawlaczyk, Krzysztof
Sato, Maki
Bręborowicz, Andrzej
Witowski, Janusz
Korybalska, Katarzyna
author_facet Kanikowska, Dominika
Malińska, Agnieszka
Mickiewicz, Agnieszka
Zawada, Agnieszka
Rutkowski, Rafał
Pawlaczyk, Krzysztof
Sato, Maki
Bręborowicz, Andrzej
Witowski, Janusz
Korybalska, Katarzyna
author_sort Kanikowska, Dominika
collection PubMed
description Context: Flaxseed has a characteristic fatty acids composition and unique phytonutrient profile that may have health-promoting properties. Objective: This study aimed to determine the effects of 10 weeks of supplementation with the flaxseed (28 g/day) on endothelial cells (EC) function, serum lipids and proinflammatory mediators in patients with mild and severe dyslipidaemia. Materials and methods: Eleven lean patients with severe dyslipidaemia treated with apheresis (group 1; 10 weeks treated in four phases: (i) ordinary diet, (ii) ordinary diet + flaxseed, (iii) ordinary diet (wash out), (iv) ordinary diet + placebo) and eleven obese patients with mild dyslipidaemia—not treated with apheresis (group 2; 10 weeks treated in two phases: (i) ordinary diet, (ii) low fat diet + flaxseed). Flaxseed was given blindly. Serum was collected at the end of each phase of the study. ECs were exposed in vitro to the medium supplemented with pooled serum taken from patients from both groups to detect their morphological changes using light and electron microscopy. ECs proliferation was also measured at the end of each study phase. Results: Serum vascular endothelial growth factor was decreased after flaxseed supplementation but only in group 1. ECs proliferation was increased after flaxseed supplementation only in obese patients. ECs exposed to medium supplemented with obese patients’ serum revealed the following cellular abnormalities: accumulation of lipid droplets, changes of rough endoplasmic reticulum and mitochondria, and flaxseed did not reverse observed changes. At the same time, flaxseed supplementation decreases total cholesterol in both tested groups, low-density lipoprotein cholesterol in group 1 and triglycerides in group 2. Conclusions: Our findings support the potential role of flaxseed in treating dyslipidaemia but indicate only a slight impact on endothelial cell function.
format Online
Article
Text
id pubmed-9315791
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93157912022-07-27 Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation Kanikowska, Dominika Malińska, Agnieszka Mickiewicz, Agnieszka Zawada, Agnieszka Rutkowski, Rafał Pawlaczyk, Krzysztof Sato, Maki Bręborowicz, Andrzej Witowski, Janusz Korybalska, Katarzyna Nutrients Article Context: Flaxseed has a characteristic fatty acids composition and unique phytonutrient profile that may have health-promoting properties. Objective: This study aimed to determine the effects of 10 weeks of supplementation with the flaxseed (28 g/day) on endothelial cells (EC) function, serum lipids and proinflammatory mediators in patients with mild and severe dyslipidaemia. Materials and methods: Eleven lean patients with severe dyslipidaemia treated with apheresis (group 1; 10 weeks treated in four phases: (i) ordinary diet, (ii) ordinary diet + flaxseed, (iii) ordinary diet (wash out), (iv) ordinary diet + placebo) and eleven obese patients with mild dyslipidaemia—not treated with apheresis (group 2; 10 weeks treated in two phases: (i) ordinary diet, (ii) low fat diet + flaxseed). Flaxseed was given blindly. Serum was collected at the end of each phase of the study. ECs were exposed in vitro to the medium supplemented with pooled serum taken from patients from both groups to detect their morphological changes using light and electron microscopy. ECs proliferation was also measured at the end of each study phase. Results: Serum vascular endothelial growth factor was decreased after flaxseed supplementation but only in group 1. ECs proliferation was increased after flaxseed supplementation only in obese patients. ECs exposed to medium supplemented with obese patients’ serum revealed the following cellular abnormalities: accumulation of lipid droplets, changes of rough endoplasmic reticulum and mitochondria, and flaxseed did not reverse observed changes. At the same time, flaxseed supplementation decreases total cholesterol in both tested groups, low-density lipoprotein cholesterol in group 1 and triglycerides in group 2. Conclusions: Our findings support the potential role of flaxseed in treating dyslipidaemia but indicate only a slight impact on endothelial cell function. MDPI 2022-07-13 /pmc/articles/PMC9315791/ /pubmed/35889835 http://dx.doi.org/10.3390/nu14142879 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kanikowska, Dominika
Malińska, Agnieszka
Mickiewicz, Agnieszka
Zawada, Agnieszka
Rutkowski, Rafał
Pawlaczyk, Krzysztof
Sato, Maki
Bręborowicz, Andrzej
Witowski, Janusz
Korybalska, Katarzyna
Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
title Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
title_full Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
title_fullStr Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
title_full_unstemmed Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
title_short Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
title_sort effect of flaxseed (linum usitatissimum l.) supplementation on vascular endothelial cell morphology and function in patients with dyslipidaemia—a preliminary observation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315791/
https://www.ncbi.nlm.nih.gov/pubmed/35889835
http://dx.doi.org/10.3390/nu14142879
work_keys_str_mv AT kanikowskadominika effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT malinskaagnieszka effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT mickiewiczagnieszka effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT zawadaagnieszka effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT rutkowskirafał effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT pawlaczykkrzysztof effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT satomaki effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT breborowiczandrzej effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT witowskijanusz effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation
AT korybalskakatarzyna effectofflaxseedlinumusitatissimumlsupplementationonvascularendothelialcellmorphologyandfunctioninpatientswithdyslipidaemiaapreliminaryobservation