Prevalence and Risk Factors for Bartonella spp. and Haemoplasma Infections in Cats from Greece

SIMPLE SUMMARY: Bartonellosis and haemoplasmosis are diseases with global impact on the health of domestic cats and of zoonotic importance. This is the first study investigating the risk factors for Bartonella spp. and haemoplasma species infections in cats from Greece. In addition, this study determined the serologic and molecular prevalence of Bartonella spp. and haemoplasma species infections in different populations of cats living in different regions of Greece. A total of 452 cats were enrolled into the study. Blood was collected from each cat for the serological detection of Bartonella henselae antibodies and the molecular detection of Bartonella spp. and haemoplasma species infections. Overall, the seroprevalence of B. henselae was 35.4%, while the molecular prevalence of Bartonella spp. and haemoplasma species was 2.9% and 19%, respectively. The results of this study indicate that cats with partial or exclusive outdoor access and cats with flea infestation are at the greatest risk for B. henselae seropositivity. Furthermore, cats living in warmer regions such as Attica and Crete are at the greatest risk. Lack of ectoparasiticide use was identified as a risk factor for haemoplasma species infection. This suggests that the use of ectoparasiticides in cats may be an effective means of preventing haemoplasma species infection in cats. ABSTRACT: Bartonellosis and haemoplasmosis are vector-borne diseases with global impact on the health of domestic cats and of zoonotic importance. The aim of this study was to describe the epidemiological aspects of various populations of cats infected with Bartonella spp. or haemoplasma species. The populations evaluated included client-owned cats, stray cats and cats that live in breeding catteries in Greece. A total of 452 cats were prospectively enrolled into the study. A commercially available indirect immunofluorescence antibody testkit was used for the detection of Bartonella henselae IgG antibodies in serum. PCRs for the detection of Bartonella spp. and haemoplasma species DNA in the blood were also performed in a subgroup of 242 of the 452 cats. Risk factors for B. henselae seropositivity and infection with the haemoplasma species were determined using multivariable analysis. Overall, 160 (35.4%) of the 452 cats were seropositive for B. henselae. Seven (2.9%) and 46 (19%) of the 242 cats were PCR-positive for Bartonella spp. and haemoplasma species, respectively. The factors associated with B. henselae seropositivity, based on multivariate analysis, included older age, outdoor access, living region and flea infestation. Non-administration of ectoparasiticides was associated with haemoplasma species infection. This study shows a high prevalence of seropositivity for B. henselae and a relatively high prevalence of infection with haemoplasma species. Therefore, it is necessary to establish optimal strategies for the prevention of Bartonella spp. and haemoplasma species infections, considering the high-risk groups of cats identified in this study.