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New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics

With the rapid and continuous emergence of antimicrobial resistance, bacterial infections became a significant global healthcare concern. One of the proposed strategies to combat multidrug-resistant pathogens is to use additional compounds, such as natural biologically active substances, as adjuvant...

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Autores principales: Woziwodzka, Anna, Krychowiak-Maśnicka, Marta, Gołuński, Grzegorz, Łosiewska, Anna, Borowik, Agnieszka, Wyrzykowski, Dariusz, Piosik, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315996/
https://www.ncbi.nlm.nih.gov/pubmed/35890171
http://dx.doi.org/10.3390/ph15070872
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author Woziwodzka, Anna
Krychowiak-Maśnicka, Marta
Gołuński, Grzegorz
Łosiewska, Anna
Borowik, Agnieszka
Wyrzykowski, Dariusz
Piosik, Jacek
author_facet Woziwodzka, Anna
Krychowiak-Maśnicka, Marta
Gołuński, Grzegorz
Łosiewska, Anna
Borowik, Agnieszka
Wyrzykowski, Dariusz
Piosik, Jacek
author_sort Woziwodzka, Anna
collection PubMed
description With the rapid and continuous emergence of antimicrobial resistance, bacterial infections became a significant global healthcare concern. One of the proposed strategies to combat multidrug-resistant pathogens is to use additional compounds, such as natural biologically active substances, as adjuvants for existing antibiotics. In this study, we investigated the potential of caffeine, the widely consumed alkaloid, to modulate the antibacterial effects of antibiotics commonly used in clinical practice. We used disc diffusion assay to evaluate the effects of caffeine on 40 antibiotics in two Staphylococcus aureus strains (methicillin-resistant and methicillin-sensitive). Based on the results of this step, we selected five antibiotics for which the greatest caffeine-induced improvements in antibacterial activity were observed, and further analyzed their interactions with caffeine using a checkerboard approach. Caffeine at concentrations of 250 µg/mL or higher halved the MIC values of ticarcillin, cefepime, gentamycin, azithromycin, and novobiocin for all gram-negative species investigated (Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii). At the highest caffeine concentrations tested (up to 16 mg/mL), decreases in MIC values were 8- to 16-fold. The obtained results prove that caffeine modulates the activity of structurally diverse antibiotics, with the most promising synergistic effects observed for cefepime and azithromycin toward gram-negative pathogens.
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spelling pubmed-93159962022-07-27 New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics Woziwodzka, Anna Krychowiak-Maśnicka, Marta Gołuński, Grzegorz Łosiewska, Anna Borowik, Agnieszka Wyrzykowski, Dariusz Piosik, Jacek Pharmaceuticals (Basel) Article With the rapid and continuous emergence of antimicrobial resistance, bacterial infections became a significant global healthcare concern. One of the proposed strategies to combat multidrug-resistant pathogens is to use additional compounds, such as natural biologically active substances, as adjuvants for existing antibiotics. In this study, we investigated the potential of caffeine, the widely consumed alkaloid, to modulate the antibacterial effects of antibiotics commonly used in clinical practice. We used disc diffusion assay to evaluate the effects of caffeine on 40 antibiotics in two Staphylococcus aureus strains (methicillin-resistant and methicillin-sensitive). Based on the results of this step, we selected five antibiotics for which the greatest caffeine-induced improvements in antibacterial activity were observed, and further analyzed their interactions with caffeine using a checkerboard approach. Caffeine at concentrations of 250 µg/mL or higher halved the MIC values of ticarcillin, cefepime, gentamycin, azithromycin, and novobiocin for all gram-negative species investigated (Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii). At the highest caffeine concentrations tested (up to 16 mg/mL), decreases in MIC values were 8- to 16-fold. The obtained results prove that caffeine modulates the activity of structurally diverse antibiotics, with the most promising synergistic effects observed for cefepime and azithromycin toward gram-negative pathogens. MDPI 2022-07-15 /pmc/articles/PMC9315996/ /pubmed/35890171 http://dx.doi.org/10.3390/ph15070872 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Woziwodzka, Anna
Krychowiak-Maśnicka, Marta
Gołuński, Grzegorz
Łosiewska, Anna
Borowik, Agnieszka
Wyrzykowski, Dariusz
Piosik, Jacek
New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
title New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
title_full New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
title_fullStr New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
title_full_unstemmed New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
title_short New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
title_sort new life of an old drug: caffeine as a modulator of antibacterial activity of commonly used antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315996/
https://www.ncbi.nlm.nih.gov/pubmed/35890171
http://dx.doi.org/10.3390/ph15070872
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