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Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2

The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation...

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Autores principales: Xie, Cangyou, Satake-Ozawa, Michiko, Rashed, Fatma, Khan, Masud, Ikeda, Masaomi, Hayashi, Shunya, Sawada, Shinichi, Sasaki, Yoshihiro, Ikeda, Tohru, Mori, Yoshiyuki, Akiyoshi, Kazunari, Aoki, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316061/
https://www.ncbi.nlm.nih.gov/pubmed/35887115
http://dx.doi.org/10.3390/ijms23147768
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author Xie, Cangyou
Satake-Ozawa, Michiko
Rashed, Fatma
Khan, Masud
Ikeda, Masaomi
Hayashi, Shunya
Sawada, Shinichi
Sasaki, Yoshihiro
Ikeda, Tohru
Mori, Yoshiyuki
Akiyoshi, Kazunari
Aoki, Kazuhiro
author_facet Xie, Cangyou
Satake-Ozawa, Michiko
Rashed, Fatma
Khan, Masud
Ikeda, Masaomi
Hayashi, Shunya
Sawada, Shinichi
Sasaki, Yoshihiro
Ikeda, Tohru
Mori, Yoshiyuki
Akiyoshi, Kazunari
Aoki, Kazuhiro
author_sort Xie, Cangyou
collection PubMed
description The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation of peptides and to allow their sustained release and activity; however, the appropriate design of CHP nanogels to conduct local bone formation needs to be developed. In the present study, we investigated the osteoconductive capacity of a newly synthesized CHP nanogel, CHPA using OP3-4 and BMP-2. We also clarified the difference between perforated and nonperforated CHPA impregnated with the two signaling molecules. Thirty-six, five-week-old male BALB/c mice were used for the calvarial defect model. The mice were euthanized at 6 weeks postoperatively. A higher cortical bone mineral content and bone formation rate were observed in the perforated scaffold in comparison to the nonperforated scaffold, especially in the OP3-4/BMP-2 combination group. The degradation rate of scaffold material in the perforated OP3-4/BMP-2 combination group was lower than that in the nonperforated group. These data suggest that perforated CHPA nanogel could lead to local bone formation induced by OP3-4 and BMP–2 and clarified the appropriate degradation rate for inducing local bone formation when CHPA nanogels are designed to be perforated.
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spelling pubmed-93160612022-07-27 Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 Xie, Cangyou Satake-Ozawa, Michiko Rashed, Fatma Khan, Masud Ikeda, Masaomi Hayashi, Shunya Sawada, Shinichi Sasaki, Yoshihiro Ikeda, Tohru Mori, Yoshiyuki Akiyoshi, Kazunari Aoki, Kazuhiro Int J Mol Sci Article The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation of peptides and to allow their sustained release and activity; however, the appropriate design of CHP nanogels to conduct local bone formation needs to be developed. In the present study, we investigated the osteoconductive capacity of a newly synthesized CHP nanogel, CHPA using OP3-4 and BMP-2. We also clarified the difference between perforated and nonperforated CHPA impregnated with the two signaling molecules. Thirty-six, five-week-old male BALB/c mice were used for the calvarial defect model. The mice were euthanized at 6 weeks postoperatively. A higher cortical bone mineral content and bone formation rate were observed in the perforated scaffold in comparison to the nonperforated scaffold, especially in the OP3-4/BMP-2 combination group. The degradation rate of scaffold material in the perforated OP3-4/BMP-2 combination group was lower than that in the nonperforated group. These data suggest that perforated CHPA nanogel could lead to local bone formation induced by OP3-4 and BMP–2 and clarified the appropriate degradation rate for inducing local bone formation when CHPA nanogels are designed to be perforated. MDPI 2022-07-14 /pmc/articles/PMC9316061/ /pubmed/35887115 http://dx.doi.org/10.3390/ijms23147768 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Cangyou
Satake-Ozawa, Michiko
Rashed, Fatma
Khan, Masud
Ikeda, Masaomi
Hayashi, Shunya
Sawada, Shinichi
Sasaki, Yoshihiro
Ikeda, Tohru
Mori, Yoshiyuki
Akiyoshi, Kazunari
Aoki, Kazuhiro
Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
title Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
title_full Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
title_fullStr Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
title_full_unstemmed Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
title_short Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
title_sort perforated hydrogels consisting of cholesterol-bearing pullulan (chp) nanogels: a newly designed scaffold for bone regeneration induced by rankl-binding peptides and bmp-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316061/
https://www.ncbi.nlm.nih.gov/pubmed/35887115
http://dx.doi.org/10.3390/ijms23147768
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