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Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2
The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316061/ https://www.ncbi.nlm.nih.gov/pubmed/35887115 http://dx.doi.org/10.3390/ijms23147768 |
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author | Xie, Cangyou Satake-Ozawa, Michiko Rashed, Fatma Khan, Masud Ikeda, Masaomi Hayashi, Shunya Sawada, Shinichi Sasaki, Yoshihiro Ikeda, Tohru Mori, Yoshiyuki Akiyoshi, Kazunari Aoki, Kazuhiro |
author_facet | Xie, Cangyou Satake-Ozawa, Michiko Rashed, Fatma Khan, Masud Ikeda, Masaomi Hayashi, Shunya Sawada, Shinichi Sasaki, Yoshihiro Ikeda, Tohru Mori, Yoshiyuki Akiyoshi, Kazunari Aoki, Kazuhiro |
author_sort | Xie, Cangyou |
collection | PubMed |
description | The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation of peptides and to allow their sustained release and activity; however, the appropriate design of CHP nanogels to conduct local bone formation needs to be developed. In the present study, we investigated the osteoconductive capacity of a newly synthesized CHP nanogel, CHPA using OP3-4 and BMP-2. We also clarified the difference between perforated and nonperforated CHPA impregnated with the two signaling molecules. Thirty-six, five-week-old male BALB/c mice were used for the calvarial defect model. The mice were euthanized at 6 weeks postoperatively. A higher cortical bone mineral content and bone formation rate were observed in the perforated scaffold in comparison to the nonperforated scaffold, especially in the OP3-4/BMP-2 combination group. The degradation rate of scaffold material in the perforated OP3-4/BMP-2 combination group was lower than that in the nonperforated group. These data suggest that perforated CHPA nanogel could lead to local bone formation induced by OP3-4 and BMP–2 and clarified the appropriate degradation rate for inducing local bone formation when CHPA nanogels are designed to be perforated. |
format | Online Article Text |
id | pubmed-9316061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93160612022-07-27 Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 Xie, Cangyou Satake-Ozawa, Michiko Rashed, Fatma Khan, Masud Ikeda, Masaomi Hayashi, Shunya Sawada, Shinichi Sasaki, Yoshihiro Ikeda, Tohru Mori, Yoshiyuki Akiyoshi, Kazunari Aoki, Kazuhiro Int J Mol Sci Article The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation of peptides and to allow their sustained release and activity; however, the appropriate design of CHP nanogels to conduct local bone formation needs to be developed. In the present study, we investigated the osteoconductive capacity of a newly synthesized CHP nanogel, CHPA using OP3-4 and BMP-2. We also clarified the difference between perforated and nonperforated CHPA impregnated with the two signaling molecules. Thirty-six, five-week-old male BALB/c mice were used for the calvarial defect model. The mice were euthanized at 6 weeks postoperatively. A higher cortical bone mineral content and bone formation rate were observed in the perforated scaffold in comparison to the nonperforated scaffold, especially in the OP3-4/BMP-2 combination group. The degradation rate of scaffold material in the perforated OP3-4/BMP-2 combination group was lower than that in the nonperforated group. These data suggest that perforated CHPA nanogel could lead to local bone formation induced by OP3-4 and BMP–2 and clarified the appropriate degradation rate for inducing local bone formation when CHPA nanogels are designed to be perforated. MDPI 2022-07-14 /pmc/articles/PMC9316061/ /pubmed/35887115 http://dx.doi.org/10.3390/ijms23147768 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Cangyou Satake-Ozawa, Michiko Rashed, Fatma Khan, Masud Ikeda, Masaomi Hayashi, Shunya Sawada, Shinichi Sasaki, Yoshihiro Ikeda, Tohru Mori, Yoshiyuki Akiyoshi, Kazunari Aoki, Kazuhiro Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 |
title | Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 |
title_full | Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 |
title_fullStr | Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 |
title_full_unstemmed | Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 |
title_short | Perforated Hydrogels Consisting of Cholesterol-Bearing Pullulan (CHP) Nanogels: A Newly Designed Scaffold for Bone Regeneration Induced by RANKL-Binding Peptides and BMP-2 |
title_sort | perforated hydrogels consisting of cholesterol-bearing pullulan (chp) nanogels: a newly designed scaffold for bone regeneration induced by rankl-binding peptides and bmp-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316061/ https://www.ncbi.nlm.nih.gov/pubmed/35887115 http://dx.doi.org/10.3390/ijms23147768 |
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