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Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells

Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier...

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Autores principales: Kong, Junhua, Garcia, Virginie, Zehraoui, Enric, Stammitti, Linda, Hilbert, Ghislaine, Renaud, Christel, Maury, Stéphane, Delaunay, Alain, Cluzet, Stéphanie, Lecourieux, Fatma, Lecourieux, David, Teyssier, Emeline, Gallusci, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316115/
https://www.ncbi.nlm.nih.gov/pubmed/35886036
http://dx.doi.org/10.3390/genes13071256
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author Kong, Junhua
Garcia, Virginie
Zehraoui, Enric
Stammitti, Linda
Hilbert, Ghislaine
Renaud, Christel
Maury, Stéphane
Delaunay, Alain
Cluzet, Stéphanie
Lecourieux, Fatma
Lecourieux, David
Teyssier, Emeline
Gallusci, Philippe
author_facet Kong, Junhua
Garcia, Virginie
Zehraoui, Enric
Stammitti, Linda
Hilbert, Ghislaine
Renaud, Christel
Maury, Stéphane
Delaunay, Alain
Cluzet, Stéphanie
Lecourieux, Fatma
Lecourieux, David
Teyssier, Emeline
Gallusci, Philippe
author_sort Kong, Junhua
collection PubMed
description Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated.
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spelling pubmed-93161152022-07-27 Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells Kong, Junhua Garcia, Virginie Zehraoui, Enric Stammitti, Linda Hilbert, Ghislaine Renaud, Christel Maury, Stéphane Delaunay, Alain Cluzet, Stéphanie Lecourieux, Fatma Lecourieux, David Teyssier, Emeline Gallusci, Philippe Genes (Basel) Article Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated. MDPI 2022-07-15 /pmc/articles/PMC9316115/ /pubmed/35886036 http://dx.doi.org/10.3390/genes13071256 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kong, Junhua
Garcia, Virginie
Zehraoui, Enric
Stammitti, Linda
Hilbert, Ghislaine
Renaud, Christel
Maury, Stéphane
Delaunay, Alain
Cluzet, Stéphanie
Lecourieux, Fatma
Lecourieux, David
Teyssier, Emeline
Gallusci, Philippe
Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
title Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
title_full Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
title_fullStr Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
title_full_unstemmed Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
title_short Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
title_sort zebularine, a dna methylation inhibitor, activates anthocyanin accumulation in grapevine cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316115/
https://www.ncbi.nlm.nih.gov/pubmed/35886036
http://dx.doi.org/10.3390/genes13071256
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