Cargando…
Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis
Many cell lines in the embryogenic callus cannot produce somatic embryos (SEs) even if they meet the optimal SE maturation culture conditions during conifer somatic embryogenesis. This phenomenon hinders the progress of the industrial-scale reproduction of conifers. Therefore, there is an urgent nee...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316463/ https://www.ncbi.nlm.nih.gov/pubmed/35890500 http://dx.doi.org/10.3390/plants11141867 |
_version_ | 1784754818730950656 |
---|---|
author | Peng, Chunxue Gao, Fang Wang, Hao Tretyakova, Iraida Nikolaevna Nosov, Alexander Mikhaylovich Shen, Hailong Yang, Ling |
author_facet | Peng, Chunxue Gao, Fang Wang, Hao Tretyakova, Iraida Nikolaevna Nosov, Alexander Mikhaylovich Shen, Hailong Yang, Ling |
author_sort | Peng, Chunxue |
collection | PubMed |
description | Many cell lines in the embryogenic callus cannot produce somatic embryos (SEs) even if they meet the optimal SE maturation culture conditions during conifer somatic embryogenesis. This phenomenon hinders the progress of the industrial-scale reproduction of conifers. Therefore, there is an urgent need to obtain morphological and physiological markers to screen embryogenic calli in response to SE maturation conditions. To detect cell lines with high somatic embryogenesis potential during the proliferation process, we counted the number of pro-embryos and early SEs (ESEs) in different cell lines and storage substances, endogenous hormones, and polyamine contents. The results showed that the yield of P. koraiensis SEs was heavily dependent on genotype (p = 0.001). There were high levels of PE III (pro-embryo III) number, ESE number, and soluble protein content, in the response cell lines (R cell lines), which were 1.6-, 3-, and 1.1-fold those of the obstructive cell lines (B cell lines), respectively. The B cell line had high levels of starch, auxin (IAA), Put, Spd, and putrescine: spermine (Put: Spm) compared to the R cell line. In addition, the numbers of PE III, ESEs, and soluble protein content were significantly positively correlated with SE yield. In contrast, the contents of starch, abscisic acid (ABA), Put, Spm, and Spd were significantly negatively correlated with SE yield. To ensure the accuracy of the results, we used nine cell lines to test the results. The PE III and ESE numbers and the Spm and Spd contents were positively correlated with SE yield, while the levels of starch, ABA, IAA, Put: Spd, and Put: Spm were negatively correlated with SE yield. Thus, we recommend using high PE III and ESEs as morphological indicators and low levels of starch, IAA, ABA, and Put: Spm as physiological markers to screen cell lines with a high somatic embryogenesis potential. In addition, we also found that the relationship between Spd, Spm, and SE yield was opposite in the two experimental results. Therefore, we speculate that the differences in Spd and Spm content are mainly affected by genotype. In conclusion, this study obtained the morphological and physiological markers of some high-somatic embryogenic cell lines by comparing the differences between nine somatic embryogenic cell lines. Our results can guide the improvement of conifer somatic embryogenesis technology and can provide a theoretical basis for accelerating the application of biotechnology in large-scale artificial breeding. |
format | Online Article Text |
id | pubmed-9316463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93164632022-07-27 Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis Peng, Chunxue Gao, Fang Wang, Hao Tretyakova, Iraida Nikolaevna Nosov, Alexander Mikhaylovich Shen, Hailong Yang, Ling Plants (Basel) Article Many cell lines in the embryogenic callus cannot produce somatic embryos (SEs) even if they meet the optimal SE maturation culture conditions during conifer somatic embryogenesis. This phenomenon hinders the progress of the industrial-scale reproduction of conifers. Therefore, there is an urgent need to obtain morphological and physiological markers to screen embryogenic calli in response to SE maturation conditions. To detect cell lines with high somatic embryogenesis potential during the proliferation process, we counted the number of pro-embryos and early SEs (ESEs) in different cell lines and storage substances, endogenous hormones, and polyamine contents. The results showed that the yield of P. koraiensis SEs was heavily dependent on genotype (p = 0.001). There were high levels of PE III (pro-embryo III) number, ESE number, and soluble protein content, in the response cell lines (R cell lines), which were 1.6-, 3-, and 1.1-fold those of the obstructive cell lines (B cell lines), respectively. The B cell line had high levels of starch, auxin (IAA), Put, Spd, and putrescine: spermine (Put: Spm) compared to the R cell line. In addition, the numbers of PE III, ESEs, and soluble protein content were significantly positively correlated with SE yield. In contrast, the contents of starch, abscisic acid (ABA), Put, Spm, and Spd were significantly negatively correlated with SE yield. To ensure the accuracy of the results, we used nine cell lines to test the results. The PE III and ESE numbers and the Spm and Spd contents were positively correlated with SE yield, while the levels of starch, ABA, IAA, Put: Spd, and Put: Spm were negatively correlated with SE yield. Thus, we recommend using high PE III and ESEs as morphological indicators and low levels of starch, IAA, ABA, and Put: Spm as physiological markers to screen cell lines with a high somatic embryogenesis potential. In addition, we also found that the relationship between Spd, Spm, and SE yield was opposite in the two experimental results. Therefore, we speculate that the differences in Spd and Spm content are mainly affected by genotype. In conclusion, this study obtained the morphological and physiological markers of some high-somatic embryogenic cell lines by comparing the differences between nine somatic embryogenic cell lines. Our results can guide the improvement of conifer somatic embryogenesis technology and can provide a theoretical basis for accelerating the application of biotechnology in large-scale artificial breeding. MDPI 2022-07-18 /pmc/articles/PMC9316463/ /pubmed/35890500 http://dx.doi.org/10.3390/plants11141867 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Peng, Chunxue Gao, Fang Wang, Hao Tretyakova, Iraida Nikolaevna Nosov, Alexander Mikhaylovich Shen, Hailong Yang, Ling Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis |
title | Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis |
title_full | Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis |
title_fullStr | Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis |
title_full_unstemmed | Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis |
title_short | Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis |
title_sort | morphological and physiological indicators for screening cell lines with high potential for somatic embryo maturation at an early stage of somatic embryogenesis in pinus koraiensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316463/ https://www.ncbi.nlm.nih.gov/pubmed/35890500 http://dx.doi.org/10.3390/plants11141867 |
work_keys_str_mv | AT pengchunxue morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis AT gaofang morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis AT wanghao morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis AT tretyakovairaidanikolaevna morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis AT nosovalexandermikhaylovich morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis AT shenhailong morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis AT yangling morphologicalandphysiologicalindicatorsforscreeningcelllineswithhighpotentialforsomaticembryomaturationatanearlystageofsomaticembryogenesisinpinuskoraiensis |