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Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections
Periprosthetic joint infection (PJI) is a troublesome clinical issue in total joint arthroplasty (TJA). Although methicillin-resistant Staphylococcus aureus (MRSA) is considered to be the most serious pathogen in PJIs, little is known about the genotypic and phenotypic characteristics of MRSA clones...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316792/ https://www.ncbi.nlm.nih.gov/pubmed/35889965 http://dx.doi.org/10.3390/pathogens11070719 |
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author | Chen, Jiun-Liang Huang, Tsung-Yu Hsu, Wei-Bin Lee, Chiang-Wen Chiang, Yao-Chang Chang, Pey-Jium Peng, Kuo-Ti |
author_facet | Chen, Jiun-Liang Huang, Tsung-Yu Hsu, Wei-Bin Lee, Chiang-Wen Chiang, Yao-Chang Chang, Pey-Jium Peng, Kuo-Ti |
author_sort | Chen, Jiun-Liang |
collection | PubMed |
description | Periprosthetic joint infection (PJI) is a troublesome clinical issue in total joint arthroplasty (TJA). Although methicillin-resistant Staphylococcus aureus (MRSA) is considered to be the most serious pathogen in PJIs, little is known about the genotypic and phenotypic characteristics of MRSA clones isolated from PJI patients. A total of 36 MRSA isolates from PJI patients were collected at the Chang-Gung Memorial Hospital in Taiwan from May 2016 to October 2019. All MRSA isolates were subjected to genome typing. The prevalence of Panton–Valentine leucocidin (PVL), the antibiotic susceptibility profile, and the biofilm formation ability were compared among different MRSA genogroups. Additionally, demographics and clinical manifestations of patients infected with different MRSA genogroups were investigated. Eight sequence types (STs) were identified among 36 isolated from PJIs. According to the incidence of MRSA genotypes in PJIs, in this study, we divided them into four groups, including ST8 (n = 10), ST59 (n = 8), ST239 (n = 11), and other STs (n = 7). For the antibiotic susceptibility testing, we found that all MRSA isolates in the ST239 group were highly resistant to ciprofloxacin, gentamicin trimethoprim-sulfamethoxazole, and levofloxacin. Additionally, ST239 MRSA also had a higher ability to form biofilm than other groups. Importantly, patients with ST239 infection typically had a fever and exhibited higher levels of inflammatory markers, including C-reactive protein (CRP) and white blood cell count (WBC). Epidemiological investigations revealed that knee PJIs were mainly attributed to infection with ST59 MRSA and increasing trends for infection with ST8 and other ST types of MRSAs in PJI patients were observed from 2016 to 2019. The identification of MRSA genotypes in PJIs may be helpful for the management of PJIs. |
format | Online Article Text |
id | pubmed-9316792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93167922022-07-27 Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections Chen, Jiun-Liang Huang, Tsung-Yu Hsu, Wei-Bin Lee, Chiang-Wen Chiang, Yao-Chang Chang, Pey-Jium Peng, Kuo-Ti Pathogens Article Periprosthetic joint infection (PJI) is a troublesome clinical issue in total joint arthroplasty (TJA). Although methicillin-resistant Staphylococcus aureus (MRSA) is considered to be the most serious pathogen in PJIs, little is known about the genotypic and phenotypic characteristics of MRSA clones isolated from PJI patients. A total of 36 MRSA isolates from PJI patients were collected at the Chang-Gung Memorial Hospital in Taiwan from May 2016 to October 2019. All MRSA isolates were subjected to genome typing. The prevalence of Panton–Valentine leucocidin (PVL), the antibiotic susceptibility profile, and the biofilm formation ability were compared among different MRSA genogroups. Additionally, demographics and clinical manifestations of patients infected with different MRSA genogroups were investigated. Eight sequence types (STs) were identified among 36 isolated from PJIs. According to the incidence of MRSA genotypes in PJIs, in this study, we divided them into four groups, including ST8 (n = 10), ST59 (n = 8), ST239 (n = 11), and other STs (n = 7). For the antibiotic susceptibility testing, we found that all MRSA isolates in the ST239 group were highly resistant to ciprofloxacin, gentamicin trimethoprim-sulfamethoxazole, and levofloxacin. Additionally, ST239 MRSA also had a higher ability to form biofilm than other groups. Importantly, patients with ST239 infection typically had a fever and exhibited higher levels of inflammatory markers, including C-reactive protein (CRP) and white blood cell count (WBC). Epidemiological investigations revealed that knee PJIs were mainly attributed to infection with ST59 MRSA and increasing trends for infection with ST8 and other ST types of MRSAs in PJI patients were observed from 2016 to 2019. The identification of MRSA genotypes in PJIs may be helpful for the management of PJIs. MDPI 2022-06-23 /pmc/articles/PMC9316792/ /pubmed/35889965 http://dx.doi.org/10.3390/pathogens11070719 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Jiun-Liang Huang, Tsung-Yu Hsu, Wei-Bin Lee, Chiang-Wen Chiang, Yao-Chang Chang, Pey-Jium Peng, Kuo-Ti Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections |
title | Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections |
title_full | Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections |
title_fullStr | Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections |
title_full_unstemmed | Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections |
title_short | Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections |
title_sort | characterization of methicillin-resistant staphylococcus aureus isolates from periprosthetic joint infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316792/ https://www.ncbi.nlm.nih.gov/pubmed/35889965 http://dx.doi.org/10.3390/pathogens11070719 |
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