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The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System

Renal fibrosis, which is characterized as progressive extracellular matrix accumulation, is a common feature of different stages of chronic kidney disease, and the degree of fibrosis is strongly associated with renal function. In clinical practice, precise understanding of the space distribution of...

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Autores principales: Li, Han, Ding, Jiarong, Zhang, Huan, Li, Maoting, Lai, Xueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316920/
https://www.ncbi.nlm.nih.gov/pubmed/35885509
http://dx.doi.org/10.3390/diagnostics12071602
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author Li, Han
Ding, Jiarong
Zhang, Huan
Li, Maoting
Lai, Xueli
author_facet Li, Han
Ding, Jiarong
Zhang, Huan
Li, Maoting
Lai, Xueli
author_sort Li, Han
collection PubMed
description Renal fibrosis, which is characterized as progressive extracellular matrix accumulation, is a common feature of different stages of chronic kidney disease, and the degree of fibrosis is strongly associated with renal function. In clinical practice, precise understanding of the space distribution of fibrosis is extremely important for the diagnosis and prognosis of renal disease. Rapid advances in terahertz (THz) technology have been made, and this technology has a broad application in bio-detection, as it can interact and measure the collective vibrations and rotations of molecular groups. It is well known that hydroxyproline (HYP) is the key component of collagen, which is synthesized by fibroblasts to maintain the extracellular matrix, and HYP content detection in tissue homogenate can be achieved by classical biochemistry method. In this study, a THz microprobe system was employed to conduct THz microspatial scanning with a resolution of 20 µm. Both the content and distribution of HYP were directly characterized by the THz absorption spectrum. The absorption intensity in the THz spectrum was used to determine HYP density in renal tissues; therefore, the fibrosis change in the kidneys can be determined using THz scanning at micrometer resolution, which provides more possibilities for precise diagnosis of renal fibrosis.
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spelling pubmed-93169202022-07-27 The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System Li, Han Ding, Jiarong Zhang, Huan Li, Maoting Lai, Xueli Diagnostics (Basel) Article Renal fibrosis, which is characterized as progressive extracellular matrix accumulation, is a common feature of different stages of chronic kidney disease, and the degree of fibrosis is strongly associated with renal function. In clinical practice, precise understanding of the space distribution of fibrosis is extremely important for the diagnosis and prognosis of renal disease. Rapid advances in terahertz (THz) technology have been made, and this technology has a broad application in bio-detection, as it can interact and measure the collective vibrations and rotations of molecular groups. It is well known that hydroxyproline (HYP) is the key component of collagen, which is synthesized by fibroblasts to maintain the extracellular matrix, and HYP content detection in tissue homogenate can be achieved by classical biochemistry method. In this study, a THz microprobe system was employed to conduct THz microspatial scanning with a resolution of 20 µm. Both the content and distribution of HYP were directly characterized by the THz absorption spectrum. The absorption intensity in the THz spectrum was used to determine HYP density in renal tissues; therefore, the fibrosis change in the kidneys can be determined using THz scanning at micrometer resolution, which provides more possibilities for precise diagnosis of renal fibrosis. MDPI 2022-06-30 /pmc/articles/PMC9316920/ /pubmed/35885509 http://dx.doi.org/10.3390/diagnostics12071602 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Han
Ding, Jiarong
Zhang, Huan
Li, Maoting
Lai, Xueli
The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System
title The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System
title_full The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System
title_fullStr The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System
title_full_unstemmed The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System
title_short The Spatial Distribution of Renal Fibrosis Investigated by Micro-probe Terahertz Spectroscopy System
title_sort spatial distribution of renal fibrosis investigated by micro-probe terahertz spectroscopy system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316920/
https://www.ncbi.nlm.nih.gov/pubmed/35885509
http://dx.doi.org/10.3390/diagnostics12071602
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