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HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil

During the 2015–2016 epidemic, Brazil was the country with the highest rate of Zika virus (ZIKV) infection in the Americas. Twenty-nine percent of pregnant women positive for ZIKV exhibited ultrasound scans with fetus anomalies. Human leukocyte antigen-G (HLA-G) exerts immunoregulatory effects by bi...

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Autores principales: de Moraes, Amarilis Giaretta, Ayo, Christiane Maria, Elpídio, Laise Nayana Sala, de Souza, Victor Hugo, Yamanaka, Aléia Harumi Uchibaba, Nogueira, Maurício Lacerda, Passos, Saulo Duarte, Brandão, Cinara Cássia, de Mattos, Luiz Carlos, do Amaral, Greicy Cezar, Neto, Quirino Alves de Lima, Visentainer, Jeane Eliete Laguila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317030/
https://www.ncbi.nlm.nih.gov/pubmed/35877415
http://dx.doi.org/10.3390/cimb44070191
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author de Moraes, Amarilis Giaretta
Ayo, Christiane Maria
Elpídio, Laise Nayana Sala
de Souza, Victor Hugo
Yamanaka, Aléia Harumi Uchibaba
Nogueira, Maurício Lacerda
Passos, Saulo Duarte
Brandão, Cinara Cássia
de Mattos, Luiz Carlos
do Amaral, Greicy Cezar
Neto, Quirino Alves de Lima
Visentainer, Jeane Eliete Laguila
author_facet de Moraes, Amarilis Giaretta
Ayo, Christiane Maria
Elpídio, Laise Nayana Sala
de Souza, Victor Hugo
Yamanaka, Aléia Harumi Uchibaba
Nogueira, Maurício Lacerda
Passos, Saulo Duarte
Brandão, Cinara Cássia
de Mattos, Luiz Carlos
do Amaral, Greicy Cezar
Neto, Quirino Alves de Lima
Visentainer, Jeane Eliete Laguila
author_sort de Moraes, Amarilis Giaretta
collection PubMed
description During the 2015–2016 epidemic, Brazil was the country with the highest rate of Zika virus (ZIKV) infection in the Americas. Twenty-nine percent of pregnant women positive for ZIKV exhibited ultrasound scans with fetus anomalies. Human leukocyte antigen-G (HLA-G) exerts immunoregulatory effects by binding to inhibitory receptors, namely LILRB1 and LILRB2, thus preventing mother–fetus rejection and vertical pathogen transmission. The binding of HLA-G to one of its receptors modulates both innate and adaptive immunity. However, in a viral infection, these molecules may behave as pathogenic mediators shifting the pregnancy environment from an anti-inflammatory profile to a pro-inflammatory phenotype. Genetic mutations might be associated with the change in phenotype. This study aimed to explore the possible role of polymorphic sites in HLA-G, LILRB1 and LILRB2 in mother–fetus ZIKV transmission. Polymorphisms were detected by direct sequencing. Differences in allele and/or genotype frequencies for each SNP analyzed among ZIKV non-transmitting and transmitting mother–child pairs, among ZIKV-transmitting and non-transmitting mothers and between ZIKV-infected and non-infected children were compared by Mid-P exact test or Yates’ correction. Significant susceptibility of ZIKV vertical transmission is suggested in ZIKV-transmitting and non-transmitting mothers and ZIKV-infected and non-infected children for LILRB1_rs1061684 T/T (p = 0.03, Pc = 0.06, OR = 12.4; p = 0.008, Pc = 0.016, OR = 16.4) and LILRB1_rs16985478 A/A (p = 0.01, Pc = 0.02, OR = 19.2; p = 0.008, Pc = 0.016, OR = 16.4). HLA-G_rs1710 (p = 0.04, Pc = 0.52, OR = 4.30) was also a susceptibility factor. LILRB2_rs386056 G/A (p = 0.02, Pc = 0.08, OR = 0.07), LILRB2_rs7247451 G/G (p = 0.01, Pc = 0.04, OR = 0.04) and HLAG_rs9380142 T/T (p = 0.04, Pc = 0.52, OR = 0.14) were suggested as protective factors against vertical transmission. The current study suggests that polymorphic sites in the LILRB1 and HLA-G genes might be associated with mother-to-child ZIKV transmission while LILRB2 might be associated with protection against ZIKV transmission in the womb in a population from the south and southeast of Brazil.
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spelling pubmed-93170302022-07-27 HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil de Moraes, Amarilis Giaretta Ayo, Christiane Maria Elpídio, Laise Nayana Sala de Souza, Victor Hugo Yamanaka, Aléia Harumi Uchibaba Nogueira, Maurício Lacerda Passos, Saulo Duarte Brandão, Cinara Cássia de Mattos, Luiz Carlos do Amaral, Greicy Cezar Neto, Quirino Alves de Lima Visentainer, Jeane Eliete Laguila Curr Issues Mol Biol Article During the 2015–2016 epidemic, Brazil was the country with the highest rate of Zika virus (ZIKV) infection in the Americas. Twenty-nine percent of pregnant women positive for ZIKV exhibited ultrasound scans with fetus anomalies. Human leukocyte antigen-G (HLA-G) exerts immunoregulatory effects by binding to inhibitory receptors, namely LILRB1 and LILRB2, thus preventing mother–fetus rejection and vertical pathogen transmission. The binding of HLA-G to one of its receptors modulates both innate and adaptive immunity. However, in a viral infection, these molecules may behave as pathogenic mediators shifting the pregnancy environment from an anti-inflammatory profile to a pro-inflammatory phenotype. Genetic mutations might be associated with the change in phenotype. This study aimed to explore the possible role of polymorphic sites in HLA-G, LILRB1 and LILRB2 in mother–fetus ZIKV transmission. Polymorphisms were detected by direct sequencing. Differences in allele and/or genotype frequencies for each SNP analyzed among ZIKV non-transmitting and transmitting mother–child pairs, among ZIKV-transmitting and non-transmitting mothers and between ZIKV-infected and non-infected children were compared by Mid-P exact test or Yates’ correction. Significant susceptibility of ZIKV vertical transmission is suggested in ZIKV-transmitting and non-transmitting mothers and ZIKV-infected and non-infected children for LILRB1_rs1061684 T/T (p = 0.03, Pc = 0.06, OR = 12.4; p = 0.008, Pc = 0.016, OR = 16.4) and LILRB1_rs16985478 A/A (p = 0.01, Pc = 0.02, OR = 19.2; p = 0.008, Pc = 0.016, OR = 16.4). HLA-G_rs1710 (p = 0.04, Pc = 0.52, OR = 4.30) was also a susceptibility factor. LILRB2_rs386056 G/A (p = 0.02, Pc = 0.08, OR = 0.07), LILRB2_rs7247451 G/G (p = 0.01, Pc = 0.04, OR = 0.04) and HLAG_rs9380142 T/T (p = 0.04, Pc = 0.52, OR = 0.14) were suggested as protective factors against vertical transmission. The current study suggests that polymorphic sites in the LILRB1 and HLA-G genes might be associated with mother-to-child ZIKV transmission while LILRB2 might be associated with protection against ZIKV transmission in the womb in a population from the south and southeast of Brazil. MDPI 2022-06-27 /pmc/articles/PMC9317030/ /pubmed/35877415 http://dx.doi.org/10.3390/cimb44070191 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Moraes, Amarilis Giaretta
Ayo, Christiane Maria
Elpídio, Laise Nayana Sala
de Souza, Victor Hugo
Yamanaka, Aléia Harumi Uchibaba
Nogueira, Maurício Lacerda
Passos, Saulo Duarte
Brandão, Cinara Cássia
de Mattos, Luiz Carlos
do Amaral, Greicy Cezar
Neto, Quirino Alves de Lima
Visentainer, Jeane Eliete Laguila
HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
title HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
title_full HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
title_fullStr HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
title_full_unstemmed HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
title_short HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil
title_sort hla-g, lilrb1 and lilrb2 variants in zika virus transmission from mother to child in a population from south and southeast of brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317030/
https://www.ncbi.nlm.nih.gov/pubmed/35877415
http://dx.doi.org/10.3390/cimb44070191
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