Cargando…

DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma

Hypermethylation of tumor suppressor genes and hypomethylation of oncogenes might be identified as possible biomarkers in gastric cancer (GC). We aimed to assess the DNA methylation status of selected genes in GC tissue samples and evaluate these genes’ prognostic importance on patient survival. Pat...

Descripción completa

Detalles Bibliográficos
Autores principales: Ignatavicius, Povilas, Dauksa, Albertas, Zilinskas, Justas, Kazokaite, Mintaute, Riauka, Romualdas, Barauskas, Giedrius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317388/
https://www.ncbi.nlm.nih.gov/pubmed/35885590
http://dx.doi.org/10.3390/diagnostics12071686
_version_ 1784755043270918144
author Ignatavicius, Povilas
Dauksa, Albertas
Zilinskas, Justas
Kazokaite, Mintaute
Riauka, Romualdas
Barauskas, Giedrius
author_facet Ignatavicius, Povilas
Dauksa, Albertas
Zilinskas, Justas
Kazokaite, Mintaute
Riauka, Romualdas
Barauskas, Giedrius
author_sort Ignatavicius, Povilas
collection PubMed
description Hypermethylation of tumor suppressor genes and hypomethylation of oncogenes might be identified as possible biomarkers in gastric cancer (GC). We aimed to assess the DNA methylation status of selected genes in GC tissue samples and evaluate these genes’ prognostic importance on patient survival. Patients (99) diagnosed with GC and who underwent gastrectomy were included. We selected a group of genes (RAD51B, GFRA3, AKR7A3, HOXA11, TUSC3, FLI1, SEZ6L, GLDC, NDRG) which may be considered as potential tumor suppressor genes and oncogenes. Methylation of the HOXA11 gene promoter was significantly more frequent in GC tumor tissue (p = 0.006) than in healthy gastric mucosa. The probability of surviving longer (71.2 months (95% CI 57–85.3) vs. 44.3 months (95% CI 34.8–53.9)) was observed with unmethylated HOXA11 promoter in cancer tissues. Survival in patients with a methylation of HOXA11 promoter either in healthy gastric mucosa or gastric cancer tissue was twice as high as in patients with a methylation of HOXA11 promoter in both healthy gastric mucosa and cancer tissue (61.2 months (95% CI 50.9–71.4) vs. 28.5 months (95% CI 20.8–36.2)). Multivariate Cox analysis revealed the HOXA11 methylation as significantly associated with patients’ survival (HR = 2.4, 95% CI 1.19–4.86). Our results suggest that the HOXA11 gene might be a potential prognostic molecular marker in patients with gastric adenocarcinoma.
format Online
Article
Text
id pubmed-9317388
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93173882022-07-27 DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma Ignatavicius, Povilas Dauksa, Albertas Zilinskas, Justas Kazokaite, Mintaute Riauka, Romualdas Barauskas, Giedrius Diagnostics (Basel) Article Hypermethylation of tumor suppressor genes and hypomethylation of oncogenes might be identified as possible biomarkers in gastric cancer (GC). We aimed to assess the DNA methylation status of selected genes in GC tissue samples and evaluate these genes’ prognostic importance on patient survival. Patients (99) diagnosed with GC and who underwent gastrectomy were included. We selected a group of genes (RAD51B, GFRA3, AKR7A3, HOXA11, TUSC3, FLI1, SEZ6L, GLDC, NDRG) which may be considered as potential tumor suppressor genes and oncogenes. Methylation of the HOXA11 gene promoter was significantly more frequent in GC tumor tissue (p = 0.006) than in healthy gastric mucosa. The probability of surviving longer (71.2 months (95% CI 57–85.3) vs. 44.3 months (95% CI 34.8–53.9)) was observed with unmethylated HOXA11 promoter in cancer tissues. Survival in patients with a methylation of HOXA11 promoter either in healthy gastric mucosa or gastric cancer tissue was twice as high as in patients with a methylation of HOXA11 promoter in both healthy gastric mucosa and cancer tissue (61.2 months (95% CI 50.9–71.4) vs. 28.5 months (95% CI 20.8–36.2)). Multivariate Cox analysis revealed the HOXA11 methylation as significantly associated with patients’ survival (HR = 2.4, 95% CI 1.19–4.86). Our results suggest that the HOXA11 gene might be a potential prognostic molecular marker in patients with gastric adenocarcinoma. MDPI 2022-07-11 /pmc/articles/PMC9317388/ /pubmed/35885590 http://dx.doi.org/10.3390/diagnostics12071686 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ignatavicius, Povilas
Dauksa, Albertas
Zilinskas, Justas
Kazokaite, Mintaute
Riauka, Romualdas
Barauskas, Giedrius
DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma
title DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma
title_full DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma
title_fullStr DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma
title_full_unstemmed DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma
title_short DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma
title_sort dna methylation of hoxa11 gene as prognostic molecular marker in human gastric adenocarcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317388/
https://www.ncbi.nlm.nih.gov/pubmed/35885590
http://dx.doi.org/10.3390/diagnostics12071686
work_keys_str_mv AT ignataviciuspovilas dnamethylationofhoxa11geneasprognosticmolecularmarkerinhumangastricadenocarcinoma
AT dauksaalbertas dnamethylationofhoxa11geneasprognosticmolecularmarkerinhumangastricadenocarcinoma
AT zilinskasjustas dnamethylationofhoxa11geneasprognosticmolecularmarkerinhumangastricadenocarcinoma
AT kazokaitemintaute dnamethylationofhoxa11geneasprognosticmolecularmarkerinhumangastricadenocarcinoma
AT riaukaromualdas dnamethylationofhoxa11geneasprognosticmolecularmarkerinhumangastricadenocarcinoma
AT barauskasgiedrius dnamethylationofhoxa11geneasprognosticmolecularmarkerinhumangastricadenocarcinoma