The Dissemination of a Single Staphylococcus aureus Strain, Spa-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms

SIMPLE SUMMARY: Staphylococcus aureus is one of the most important pathogens causing intramammary infection (IMI) in cattle. We aimed to characterize which are the specific strains that are responsible for the majority of these cases. During the one-year survey, S. aureus was identified in 152 dairy farms, with a total of 440 positive samples. One specific type (designated-spa t2873) was found in 284 isolates (64.5%). Notably, 25 cases were detected in one specific farm, all of which were found to be dominant spa t2873 type. By using genomic analysis, we were able to ascertain that most transmission events occurred within the same milking group. Our study revealed the dissemination of a single strain to the majority of Israeli dairy farms. The possibility of inter-farm transmission should be monitored and prevented. ABSTRACT: Staphylococcus aureus is one of the most important pathogens causing intramammary infection (IMI) in dairy herds. The goals of this study were (1) to describe the prevalence of S. aureus in Israeli dairy farms; (2) to characterize the spa-based clonal structure of mastitis-related S. aureus isolates; (3) to analyze the transmission network of a large outbreak within a single farm and (4) to characterize the virulence factors of the outbreak strain. The prevalence and the molecular survey were performed on all Israeli IMI-related isolates, 9.2019–8.2020. Molecular methods included spa-typing for the survey and whole-genome sequencing (WGS) for the investigation of the farm ‘A’ outbreak. During the one-year survey, S. aureus was identified in 152 dairy farms, with a total of 440 positive samples. The spa t2873 was found in 284 isolates (64.5%) across 112 farms (73.6%). Other common types included t529 (n = 46), t9303 (n = 34) and the methicillin-resistant S. aureus t011 (n = 11). The highest number of cases (n = 25) was detected in Farm ‘A’, all of which were found to be spa t2873. Phylogenetic analysis confirmed that most transmission events occurred within the same milking group, and inter-group transmission was due to the transfer of cows between groups or due to consecutive milking order. The spa t2873 strain contained putative virulence genes, including various intracellular and collagen adhesion proteins. Our study revealed the dissemination of the t2873 strain to the majority of Israeli dairy farms. The possibility of inter-farm transmission should be monitored and prevented.