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Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate

Prorocentrum lima is a global benthic dinoflagellate that produces diarrhetic shellfish poisoning (DSP) toxins, which can be ingested by filter-feeding bivalves, and eventually pose a great threat to human health through food chain. After being exposed to P. lima, different bivalves may accumulate v...

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Autores principales: Ye, Mei-Hua, Li, Da-Wei, Cai, Qiu-Die, Jiao, Yu-Hu, Liu, Yang, Li, Hong-Ye, Yang, Wei-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317551/
https://www.ncbi.nlm.nih.gov/pubmed/35878199
http://dx.doi.org/10.3390/toxins14070461
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author Ye, Mei-Hua
Li, Da-Wei
Cai, Qiu-Die
Jiao, Yu-Hu
Liu, Yang
Li, Hong-Ye
Yang, Wei-Dong
author_facet Ye, Mei-Hua
Li, Da-Wei
Cai, Qiu-Die
Jiao, Yu-Hu
Liu, Yang
Li, Hong-Ye
Yang, Wei-Dong
author_sort Ye, Mei-Hua
collection PubMed
description Prorocentrum lima is a global benthic dinoflagellate that produces diarrhetic shellfish poisoning (DSP) toxins, which can be ingested by filter-feeding bivalves, and eventually pose a great threat to human health through food chain. After being exposed to P. lima, different bivalves may accumulate various levels of DSP toxins and display different toxic responses. However, the underlying mechanism remains unclear. Here, we found that the content of okadaic acid-equivalents (OA-eq) varied in the digestive glands of the three bivalves including Crassostrea gigas, Mytilus coruscus and Tegillarca granosa after P. lima exposure. The degree of esterification of OA-eq in the three bivalves were opposite to the accumulation of OA-eq. The digestive gland tissues of the three bivalve species were damaged to different degrees. The transcriptional induction of Nrf2 targeted genes such as ABCB1 and GPx indicates the functionality of Nrf2 pathway against DSP toxins in bivalves. The oyster could protect against DSP toxins mainly through ABC transporters and esterification, while the mussel and clam reduce the damage induced by DSP toxins mainly by regulating the expression of antioxidant genes. Our findings may provide some explanations for the difference in toxic response to DSP toxins in different shellfish.
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spelling pubmed-93175512022-07-27 Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate Ye, Mei-Hua Li, Da-Wei Cai, Qiu-Die Jiao, Yu-Hu Liu, Yang Li, Hong-Ye Yang, Wei-Dong Toxins (Basel) Article Prorocentrum lima is a global benthic dinoflagellate that produces diarrhetic shellfish poisoning (DSP) toxins, which can be ingested by filter-feeding bivalves, and eventually pose a great threat to human health through food chain. After being exposed to P. lima, different bivalves may accumulate various levels of DSP toxins and display different toxic responses. However, the underlying mechanism remains unclear. Here, we found that the content of okadaic acid-equivalents (OA-eq) varied in the digestive glands of the three bivalves including Crassostrea gigas, Mytilus coruscus and Tegillarca granosa after P. lima exposure. The degree of esterification of OA-eq in the three bivalves were opposite to the accumulation of OA-eq. The digestive gland tissues of the three bivalve species were damaged to different degrees. The transcriptional induction of Nrf2 targeted genes such as ABCB1 and GPx indicates the functionality of Nrf2 pathway against DSP toxins in bivalves. The oyster could protect against DSP toxins mainly through ABC transporters and esterification, while the mussel and clam reduce the damage induced by DSP toxins mainly by regulating the expression of antioxidant genes. Our findings may provide some explanations for the difference in toxic response to DSP toxins in different shellfish. MDPI 2022-07-05 /pmc/articles/PMC9317551/ /pubmed/35878199 http://dx.doi.org/10.3390/toxins14070461 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ye, Mei-Hua
Li, Da-Wei
Cai, Qiu-Die
Jiao, Yu-Hu
Liu, Yang
Li, Hong-Ye
Yang, Wei-Dong
Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate
title Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate
title_full Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate
title_fullStr Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate
title_full_unstemmed Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate
title_short Toxic Responses of Different Shellfish Species after Exposure to Prorocentrum lima, a DSP Toxins Producing Dinoflagellate
title_sort toxic responses of different shellfish species after exposure to prorocentrum lima, a dsp toxins producing dinoflagellate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317551/
https://www.ncbi.nlm.nih.gov/pubmed/35878199
http://dx.doi.org/10.3390/toxins14070461
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