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β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans

Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to...

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Autores principales: Lima, Tânia, Gunnarsson, Stefán B., Coelho, Elisabete, Evtuguin, Dmitry V., Correia, Alexandra, Coimbra, Manuel A., Cedervall, Tommy, Vilanova, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317568/
https://www.ncbi.nlm.nih.gov/pubmed/35889700
http://dx.doi.org/10.3390/nano12142475
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author Lima, Tânia
Gunnarsson, Stefán B.
Coelho, Elisabete
Evtuguin, Dmitry V.
Correia, Alexandra
Coimbra, Manuel A.
Cedervall, Tommy
Vilanova, Manuel
author_facet Lima, Tânia
Gunnarsson, Stefán B.
Coelho, Elisabete
Evtuguin, Dmitry V.
Correia, Alexandra
Coimbra, Manuel A.
Cedervall, Tommy
Vilanova, Manuel
author_sort Lima, Tânia
collection PubMed
description Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to fungal elimination. Nevertheless, the elicited inflammatory response may be deleterious to the host by causing excessive tissue damage. We developed a nanoparticle-based approach to modulate the host deleterious inflammatory consequences of fungal infection by using β1,3-glucan-functionalized polystyrene (β-Glc-PS) nanoparticles. β-Glc-PS nanoparticles decreased the levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-12p40 detected in in vitro culture supernatants of bone marrow-derived dendritic cells and macrophage challenged with C. albicans cells. Moreover, β-Glc-PS nanoparticles impaired the production of reactive oxygen species by bone marrow-derived dendritic cells incubated with C. albicans. This immunomodulatory effect was dependent on the nanoparticle size. Overall, β-Glc-PS nanoparticles reduced the proinflammatory response elicited by fungal cells in mononuclear phagocytes, setting the basis for a targeted therapy aimed at protecting the host by lowering the inflammatory cost of infection.
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spelling pubmed-93175682022-07-27 β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans Lima, Tânia Gunnarsson, Stefán B. Coelho, Elisabete Evtuguin, Dmitry V. Correia, Alexandra Coimbra, Manuel A. Cedervall, Tommy Vilanova, Manuel Nanomaterials (Basel) Article Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to fungal elimination. Nevertheless, the elicited inflammatory response may be deleterious to the host by causing excessive tissue damage. We developed a nanoparticle-based approach to modulate the host deleterious inflammatory consequences of fungal infection by using β1,3-glucan-functionalized polystyrene (β-Glc-PS) nanoparticles. β-Glc-PS nanoparticles decreased the levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-12p40 detected in in vitro culture supernatants of bone marrow-derived dendritic cells and macrophage challenged with C. albicans cells. Moreover, β-Glc-PS nanoparticles impaired the production of reactive oxygen species by bone marrow-derived dendritic cells incubated with C. albicans. This immunomodulatory effect was dependent on the nanoparticle size. Overall, β-Glc-PS nanoparticles reduced the proinflammatory response elicited by fungal cells in mononuclear phagocytes, setting the basis for a targeted therapy aimed at protecting the host by lowering the inflammatory cost of infection. MDPI 2022-07-19 /pmc/articles/PMC9317568/ /pubmed/35889700 http://dx.doi.org/10.3390/nano12142475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Tânia
Gunnarsson, Stefán B.
Coelho, Elisabete
Evtuguin, Dmitry V.
Correia, Alexandra
Coimbra, Manuel A.
Cedervall, Tommy
Vilanova, Manuel
β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
title β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
title_full β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
title_fullStr β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
title_full_unstemmed β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
title_short β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
title_sort β-glucan-functionalized nanoparticles down-modulate the proinflammatory response of mononuclear phagocytes challenged with candida albicans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317568/
https://www.ncbi.nlm.nih.gov/pubmed/35889700
http://dx.doi.org/10.3390/nano12142475
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