Cargando…
β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317568/ https://www.ncbi.nlm.nih.gov/pubmed/35889700 http://dx.doi.org/10.3390/nano12142475 |
_version_ | 1784755088863002624 |
---|---|
author | Lima, Tânia Gunnarsson, Stefán B. Coelho, Elisabete Evtuguin, Dmitry V. Correia, Alexandra Coimbra, Manuel A. Cedervall, Tommy Vilanova, Manuel |
author_facet | Lima, Tânia Gunnarsson, Stefán B. Coelho, Elisabete Evtuguin, Dmitry V. Correia, Alexandra Coimbra, Manuel A. Cedervall, Tommy Vilanova, Manuel |
author_sort | Lima, Tânia |
collection | PubMed |
description | Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to fungal elimination. Nevertheless, the elicited inflammatory response may be deleterious to the host by causing excessive tissue damage. We developed a nanoparticle-based approach to modulate the host deleterious inflammatory consequences of fungal infection by using β1,3-glucan-functionalized polystyrene (β-Glc-PS) nanoparticles. β-Glc-PS nanoparticles decreased the levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-12p40 detected in in vitro culture supernatants of bone marrow-derived dendritic cells and macrophage challenged with C. albicans cells. Moreover, β-Glc-PS nanoparticles impaired the production of reactive oxygen species by bone marrow-derived dendritic cells incubated with C. albicans. This immunomodulatory effect was dependent on the nanoparticle size. Overall, β-Glc-PS nanoparticles reduced the proinflammatory response elicited by fungal cells in mononuclear phagocytes, setting the basis for a targeted therapy aimed at protecting the host by lowering the inflammatory cost of infection. |
format | Online Article Text |
id | pubmed-9317568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93175682022-07-27 β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans Lima, Tânia Gunnarsson, Stefán B. Coelho, Elisabete Evtuguin, Dmitry V. Correia, Alexandra Coimbra, Manuel A. Cedervall, Tommy Vilanova, Manuel Nanomaterials (Basel) Article Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to fungal elimination. Nevertheless, the elicited inflammatory response may be deleterious to the host by causing excessive tissue damage. We developed a nanoparticle-based approach to modulate the host deleterious inflammatory consequences of fungal infection by using β1,3-glucan-functionalized polystyrene (β-Glc-PS) nanoparticles. β-Glc-PS nanoparticles decreased the levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-12p40 detected in in vitro culture supernatants of bone marrow-derived dendritic cells and macrophage challenged with C. albicans cells. Moreover, β-Glc-PS nanoparticles impaired the production of reactive oxygen species by bone marrow-derived dendritic cells incubated with C. albicans. This immunomodulatory effect was dependent on the nanoparticle size. Overall, β-Glc-PS nanoparticles reduced the proinflammatory response elicited by fungal cells in mononuclear phagocytes, setting the basis for a targeted therapy aimed at protecting the host by lowering the inflammatory cost of infection. MDPI 2022-07-19 /pmc/articles/PMC9317568/ /pubmed/35889700 http://dx.doi.org/10.3390/nano12142475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lima, Tânia Gunnarsson, Stefán B. Coelho, Elisabete Evtuguin, Dmitry V. Correia, Alexandra Coimbra, Manuel A. Cedervall, Tommy Vilanova, Manuel β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans |
title | β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans |
title_full | β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans |
title_fullStr | β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans |
title_full_unstemmed | β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans |
title_short | β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans |
title_sort | β-glucan-functionalized nanoparticles down-modulate the proinflammatory response of mononuclear phagocytes challenged with candida albicans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317568/ https://www.ncbi.nlm.nih.gov/pubmed/35889700 http://dx.doi.org/10.3390/nano12142475 |
work_keys_str_mv | AT limatania bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT gunnarssonstefanb bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT coelhoelisabete bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT evtuguindmitryv bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT correiaalexandra bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT coimbramanuela bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT cedervalltommy bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans AT vilanovamanuel bglucanfunctionalizednanoparticlesdownmodulatetheproinflammatoryresponseofmononuclearphagocyteschallengedwithcandidaalbicans |