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Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress

We aimed to investigate the cardioprotective effects of ethanolic Melissa officinalis L. extract (ME) in the rat model of myocardial ischemia/reperfusion (I/R) injury. Thirty-two Wistar rats were randomly divided into a CTRL non-treated control group with myocardial I/R injury and three experimental...

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Autores principales: Draginic, Nevena, Milosavljevic, Isidora, Andjic, Marijana, Jeremic, Jovana, Nikolic, Marina, Sretenovic, Jasmina, Kocovic, Aleksandar, Srejovic, Ivan, Zivkovic, Vladimir, Bolevich, Sergey, Bolevich, Stefani, Curcic, Svetlana, Jakovljevic, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317599/
https://www.ncbi.nlm.nih.gov/pubmed/35890139
http://dx.doi.org/10.3390/ph15070840
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author Draginic, Nevena
Milosavljevic, Isidora
Andjic, Marijana
Jeremic, Jovana
Nikolic, Marina
Sretenovic, Jasmina
Kocovic, Aleksandar
Srejovic, Ivan
Zivkovic, Vladimir
Bolevich, Sergey
Bolevich, Stefani
Curcic, Svetlana
Jakovljevic, Vladimir
author_facet Draginic, Nevena
Milosavljevic, Isidora
Andjic, Marijana
Jeremic, Jovana
Nikolic, Marina
Sretenovic, Jasmina
Kocovic, Aleksandar
Srejovic, Ivan
Zivkovic, Vladimir
Bolevich, Sergey
Bolevich, Stefani
Curcic, Svetlana
Jakovljevic, Vladimir
author_sort Draginic, Nevena
collection PubMed
description We aimed to investigate the cardioprotective effects of ethanolic Melissa officinalis L. extract (ME) in the rat model of myocardial ischemia/reperfusion (I/R) injury. Thirty-two Wistar rats were randomly divided into a CTRL non-treated control group with myocardial I/R injury and three experimental groups of rats treated with 50, 100, or 200 mg/kg of ME for 7 days per os. Afterward, hearts were isolated, and cardiodynamic function was assessed via the Langendorff model of global 20 min ischemia and 30 min reperfusion. Oxidative stress parameters were determined spectrophotometrically from the samples of coronary venous effluent (O(2)(−), H(2)O(2), TBARS, and NO(2)(−),) and heart tissue homogenate (TBARS, NO(2)(−), SOD, and CAT). H/E and Picrosirius red staining were used to examine cardiac architecture and cardiac collagen content. ME improved cardiodynamic parameters and achieved to preserve cardiac architecture after I/R injury and to decrease fibrosis, especially in the ME200 group compared to CTRL. ME200 and ME100 markedly decreased prooxidants TBARS, O(2)(−), and H(2)O(2) while increasing NO(2)(−). Hereby, we confirmed the ME`s ability to save the heart from I/R induced damage, even after short-term preconditioning in terms of preserving cardiodynamic alterations, cardiac architecture, fibrosis, and suppressing oxidative stress, especially in dose of 200 mg/kg.
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spelling pubmed-93175992022-07-27 Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress Draginic, Nevena Milosavljevic, Isidora Andjic, Marijana Jeremic, Jovana Nikolic, Marina Sretenovic, Jasmina Kocovic, Aleksandar Srejovic, Ivan Zivkovic, Vladimir Bolevich, Sergey Bolevich, Stefani Curcic, Svetlana Jakovljevic, Vladimir Pharmaceuticals (Basel) Article We aimed to investigate the cardioprotective effects of ethanolic Melissa officinalis L. extract (ME) in the rat model of myocardial ischemia/reperfusion (I/R) injury. Thirty-two Wistar rats were randomly divided into a CTRL non-treated control group with myocardial I/R injury and three experimental groups of rats treated with 50, 100, or 200 mg/kg of ME for 7 days per os. Afterward, hearts were isolated, and cardiodynamic function was assessed via the Langendorff model of global 20 min ischemia and 30 min reperfusion. Oxidative stress parameters were determined spectrophotometrically from the samples of coronary venous effluent (O(2)(−), H(2)O(2), TBARS, and NO(2)(−),) and heart tissue homogenate (TBARS, NO(2)(−), SOD, and CAT). H/E and Picrosirius red staining were used to examine cardiac architecture and cardiac collagen content. ME improved cardiodynamic parameters and achieved to preserve cardiac architecture after I/R injury and to decrease fibrosis, especially in the ME200 group compared to CTRL. ME200 and ME100 markedly decreased prooxidants TBARS, O(2)(−), and H(2)O(2) while increasing NO(2)(−). Hereby, we confirmed the ME`s ability to save the heart from I/R induced damage, even after short-term preconditioning in terms of preserving cardiodynamic alterations, cardiac architecture, fibrosis, and suppressing oxidative stress, especially in dose of 200 mg/kg. MDPI 2022-07-08 /pmc/articles/PMC9317599/ /pubmed/35890139 http://dx.doi.org/10.3390/ph15070840 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Draginic, Nevena
Milosavljevic, Isidora
Andjic, Marijana
Jeremic, Jovana
Nikolic, Marina
Sretenovic, Jasmina
Kocovic, Aleksandar
Srejovic, Ivan
Zivkovic, Vladimir
Bolevich, Sergey
Bolevich, Stefani
Curcic, Svetlana
Jakovljevic, Vladimir
Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress
title Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress
title_full Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress
title_fullStr Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress
title_full_unstemmed Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress
title_short Short-Term Administration of Lemon Balm Extract Ameliorates Myocardial Ischemia/Reperfusion Injury: Focus on Oxidative Stress
title_sort short-term administration of lemon balm extract ameliorates myocardial ischemia/reperfusion injury: focus on oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317599/
https://www.ncbi.nlm.nih.gov/pubmed/35890139
http://dx.doi.org/10.3390/ph15070840
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