Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid Macrosiphoniella sanborni

SIMPLE SUMMARY: The olfactory system is key for insects to receive external chemical signals, and various chemosensory genes are involved in this process. Thus, research focused on the olfactory mechanisms of insects can provide theoretical guidance for the development of effective green pest-control measures. In this study, an antennal transcriptome analysis of the chrysanthemum aphid (Macrosiphoniella sanborni) was conducted to identify putative chemosensory genes. The relative relationships of chemosensory genes between chrysanthemum aphids and other aphid species were analyzed. Then, the wing-specific and odor-specific expression profiles of these candidate genes were examined. This study contributes to our understanding of the olfactory system and lays the foundation for functional studies of the chemoreception mechanism in M. sanborni. ABSTRACT: As one of the most destructive oligophagous pests, the chrysanthemum aphid (Macrosiphoniella sanborni) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of M. sanborni. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of M. sanborni adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, MsanOBP9, MsanOR2, MsanOR4, MsanOR43b-1, MsanCSP1, MsanCSP2, MsanCSP4, MsanIR25a and MsanIR40a in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in M. sanborni.