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Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo
Mulberry (Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxid...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318041/ https://www.ncbi.nlm.nih.gov/pubmed/35887036 http://dx.doi.org/10.3390/ijms23147694 |
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author | Lin, Ziwei Gan, Tiantian Huang, Yanzhen Bao, Lijun Liu, Shuang Cui, Xiaopeng Wang, Hexin Jiao, Feng Zhang, Minjuan Su, Chao Qian, Yonghua |
author_facet | Lin, Ziwei Gan, Tiantian Huang, Yanzhen Bao, Lijun Liu, Shuang Cui, Xiaopeng Wang, Hexin Jiao, Feng Zhang, Minjuan Su, Chao Qian, Yonghua |
author_sort | Lin, Ziwei |
collection | PubMed |
description | Mulberry (Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, and 75%) and explore its pharmacological properties. Three types of MLFs inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in lipopolysaccharide (LPS)-induced RAW 264.7 cells. All MLFs boosted the antioxidative capacity by decreasing the reactive oxygen species (ROS) production and the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and improving the metal ion chelating activity and reducing power. The results revealed that the MLFs eluted by 30% ethanol exhibited the best anti-inflammatory and antioxidative activities. A nontargeted metabolomic analysis was used to analyze 24 types of differential flavonoids between the MLFs. Quercetin, kaempferol, and their derivatives in 30%MLF were more abundant than the other two MLFs. Furthermore, we evaluated the pharmacological activities of 30%MLF in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. The 30%MLF could alleviate the clinical symptoms, reduce the secretion of inflammatory cytokines, and inhibit the activation of the inflammatory pathway in DSS-induced colitis mice. This study will provide valuable information for the development of MLFs eluted by 30% ethanol as a functional food. |
format | Online Article Text |
id | pubmed-9318041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93180412022-07-27 Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo Lin, Ziwei Gan, Tiantian Huang, Yanzhen Bao, Lijun Liu, Shuang Cui, Xiaopeng Wang, Hexin Jiao, Feng Zhang, Minjuan Su, Chao Qian, Yonghua Int J Mol Sci Article Mulberry (Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, and 75%) and explore its pharmacological properties. Three types of MLFs inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in lipopolysaccharide (LPS)-induced RAW 264.7 cells. All MLFs boosted the antioxidative capacity by decreasing the reactive oxygen species (ROS) production and the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and improving the metal ion chelating activity and reducing power. The results revealed that the MLFs eluted by 30% ethanol exhibited the best anti-inflammatory and antioxidative activities. A nontargeted metabolomic analysis was used to analyze 24 types of differential flavonoids between the MLFs. Quercetin, kaempferol, and their derivatives in 30%MLF were more abundant than the other two MLFs. Furthermore, we evaluated the pharmacological activities of 30%MLF in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. The 30%MLF could alleviate the clinical symptoms, reduce the secretion of inflammatory cytokines, and inhibit the activation of the inflammatory pathway in DSS-induced colitis mice. This study will provide valuable information for the development of MLFs eluted by 30% ethanol as a functional food. MDPI 2022-07-12 /pmc/articles/PMC9318041/ /pubmed/35887036 http://dx.doi.org/10.3390/ijms23147694 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lin, Ziwei Gan, Tiantian Huang, Yanzhen Bao, Lijun Liu, Shuang Cui, Xiaopeng Wang, Hexin Jiao, Feng Zhang, Minjuan Su, Chao Qian, Yonghua Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
title | Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
title_full | Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
title_fullStr | Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
title_full_unstemmed | Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
title_short | Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
title_sort | anti-inflammatory activity of mulberry leaf flavonoids in vitro and in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318041/ https://www.ncbi.nlm.nih.gov/pubmed/35887036 http://dx.doi.org/10.3390/ijms23147694 |
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