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Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia

Chagas disease (CD) is one of the leading neglected tropical diseases. In the Americas, CD is endemic in about 21 countries, but only less than 1% of the patients have access to medical treatment. Indigenous populations are particularly affected because they live in socio-economic and climate condit...

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Autores principales: Kann, Simone, Dib, Juan Carlos, Aristizabal, Andrés, Mendoza, Gustavo Concha, Lacouture, Hugo Dario Soto, Hartmann, Maria, Frickmann, Hagen, Kreienbrock, Lothar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318061/
https://www.ncbi.nlm.nih.gov/pubmed/35889145
http://dx.doi.org/10.3390/microorganisms10071427
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author Kann, Simone
Dib, Juan Carlos
Aristizabal, Andrés
Mendoza, Gustavo Concha
Lacouture, Hugo Dario Soto
Hartmann, Maria
Frickmann, Hagen
Kreienbrock, Lothar
author_facet Kann, Simone
Dib, Juan Carlos
Aristizabal, Andrés
Mendoza, Gustavo Concha
Lacouture, Hugo Dario Soto
Hartmann, Maria
Frickmann, Hagen
Kreienbrock, Lothar
author_sort Kann, Simone
collection PubMed
description Chagas disease (CD) is one of the leading neglected tropical diseases. In the Americas, CD is endemic in about 21 countries, but only less than 1% of the patients have access to medical treatment. Indigenous populations are particularly affected because they live in socio-economic and climate conditions that favor CD infections. In this study, diagnostic strategies and regional prevalence of the Chagas disease were assessed. In nine villages of the indigenous tribe Wiwa, 1134 persons were tested with a Chagas-antibody-specific rapid test (RT), two different Chagas-antibody-specific ELISAs and a Chagas-specific real-time polymerase chain reaction. The overall prevalence of CD in the villages was 35.4%, with a variation from 24.9% to 52.8% for the different communities. Rapid tests and ELISAs showed the same results in all cases. The proportion of replication-active infections, defined by positive PCR results, was 8.7%. In conclusion, the assessed indigenous population in Colombia was shown to be severely affected by CD. For a serological diagnosis, one rapid test was shown to be sufficient. Replacements of ELISAs by RT would decrease costs, increase feasibility and would relevantly help detect positive patients, especially if combined with the applied real-time PCR protocol. Real-time PCR can be considered for the detection of acute cases, outbreaks, chronic cases with re-infection/activation, as well as for therapy management and control.
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spelling pubmed-93180612022-07-27 Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia Kann, Simone Dib, Juan Carlos Aristizabal, Andrés Mendoza, Gustavo Concha Lacouture, Hugo Dario Soto Hartmann, Maria Frickmann, Hagen Kreienbrock, Lothar Microorganisms Article Chagas disease (CD) is one of the leading neglected tropical diseases. In the Americas, CD is endemic in about 21 countries, but only less than 1% of the patients have access to medical treatment. Indigenous populations are particularly affected because they live in socio-economic and climate conditions that favor CD infections. In this study, diagnostic strategies and regional prevalence of the Chagas disease were assessed. In nine villages of the indigenous tribe Wiwa, 1134 persons were tested with a Chagas-antibody-specific rapid test (RT), two different Chagas-antibody-specific ELISAs and a Chagas-specific real-time polymerase chain reaction. The overall prevalence of CD in the villages was 35.4%, with a variation from 24.9% to 52.8% for the different communities. Rapid tests and ELISAs showed the same results in all cases. The proportion of replication-active infections, defined by positive PCR results, was 8.7%. In conclusion, the assessed indigenous population in Colombia was shown to be severely affected by CD. For a serological diagnosis, one rapid test was shown to be sufficient. Replacements of ELISAs by RT would decrease costs, increase feasibility and would relevantly help detect positive patients, especially if combined with the applied real-time PCR protocol. Real-time PCR can be considered for the detection of acute cases, outbreaks, chronic cases with re-infection/activation, as well as for therapy management and control. MDPI 2022-07-14 /pmc/articles/PMC9318061/ /pubmed/35889145 http://dx.doi.org/10.3390/microorganisms10071427 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kann, Simone
Dib, Juan Carlos
Aristizabal, Andrés
Mendoza, Gustavo Concha
Lacouture, Hugo Dario Soto
Hartmann, Maria
Frickmann, Hagen
Kreienbrock, Lothar
Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia
title Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia
title_full Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia
title_fullStr Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia
title_full_unstemmed Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia
title_short Diagnosis and Prevalence of Chagas Disease in an Indigenous Population of Colombia
title_sort diagnosis and prevalence of chagas disease in an indigenous population of colombia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318061/
https://www.ncbi.nlm.nih.gov/pubmed/35889145
http://dx.doi.org/10.3390/microorganisms10071427
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