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Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
Galphimia spp. is popularly used in Mexican traditional medicine. Some populations of Galphimia exert anxiolytic and sedative effects due to the presence of the modified triterpenoids galphimines. However, the galphimine synthesis pathway has not yet been elucidated. Hence, in this study, a comparat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318123/ https://www.ncbi.nlm.nih.gov/pubmed/35890513 http://dx.doi.org/10.3390/plants11141879 |
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author | Iglesias, Dianella de Donato Capote, Marcos Méndez Tenorio, Alfonso Valdivia, Ana Victoria Gutiérrez-García, Claudia Paul, Sujay Iqbal, Hafiz M. N. Villarreal, María Luisa Sharma, Ashutosh |
author_facet | Iglesias, Dianella de Donato Capote, Marcos Méndez Tenorio, Alfonso Valdivia, Ana Victoria Gutiérrez-García, Claudia Paul, Sujay Iqbal, Hafiz M. N. Villarreal, María Luisa Sharma, Ashutosh |
author_sort | Iglesias, Dianella |
collection | PubMed |
description | Galphimia spp. is popularly used in Mexican traditional medicine. Some populations of Galphimia exert anxiolytic and sedative effects due to the presence of the modified triterpenoids galphimines. However, the galphimine synthesis pathway has not yet been elucidated. Hence, in this study, a comparative transcriptome analysis between two contrasting populations of Galphimia spp., a galphimine-producer, and a non-galphimine-producer, is performed using RNA-Seq in the Illumina Next Seq 550 platform to identify putative candidates genes that encode enzymes of this metabolic pathway. Transcriptome functional annotation was performed using the Blast2GO in levels of gene ontology. For differential expression analysis, edgeR, pheatmap, and Genie3 library were used. To validate transcriptome data, qPCR was conducted. In producer and non-producer plants of both populations of Galphimia spp., most of the transcripts were grouped in the Molecular Function level of gene ontology. A total of 680 differentially expressed transcripts between producer and non-producer plants were detected. In galphimine-producer plants, a larger number of highly expressed transcripts related to acyclic and polycyclic terpene synthesis were identified. As putative candidate genes involved in the galphimine synthesis pathway, P450 family members and enzymes with kinase activity were identified. |
format | Online Article Text |
id | pubmed-9318123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93181232022-07-27 Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects Iglesias, Dianella de Donato Capote, Marcos Méndez Tenorio, Alfonso Valdivia, Ana Victoria Gutiérrez-García, Claudia Paul, Sujay Iqbal, Hafiz M. N. Villarreal, María Luisa Sharma, Ashutosh Plants (Basel) Article Galphimia spp. is popularly used in Mexican traditional medicine. Some populations of Galphimia exert anxiolytic and sedative effects due to the presence of the modified triterpenoids galphimines. However, the galphimine synthesis pathway has not yet been elucidated. Hence, in this study, a comparative transcriptome analysis between two contrasting populations of Galphimia spp., a galphimine-producer, and a non-galphimine-producer, is performed using RNA-Seq in the Illumina Next Seq 550 platform to identify putative candidates genes that encode enzymes of this metabolic pathway. Transcriptome functional annotation was performed using the Blast2GO in levels of gene ontology. For differential expression analysis, edgeR, pheatmap, and Genie3 library were used. To validate transcriptome data, qPCR was conducted. In producer and non-producer plants of both populations of Galphimia spp., most of the transcripts were grouped in the Molecular Function level of gene ontology. A total of 680 differentially expressed transcripts between producer and non-producer plants were detected. In galphimine-producer plants, a larger number of highly expressed transcripts related to acyclic and polycyclic terpene synthesis were identified. As putative candidate genes involved in the galphimine synthesis pathway, P450 family members and enzymes with kinase activity were identified. MDPI 2022-07-20 /pmc/articles/PMC9318123/ /pubmed/35890513 http://dx.doi.org/10.3390/plants11141879 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Iglesias, Dianella de Donato Capote, Marcos Méndez Tenorio, Alfonso Valdivia, Ana Victoria Gutiérrez-García, Claudia Paul, Sujay Iqbal, Hafiz M. N. Villarreal, María Luisa Sharma, Ashutosh Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects |
title | Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects |
title_full | Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects |
title_fullStr | Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects |
title_full_unstemmed | Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects |
title_short | Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects |
title_sort | identification of putative candidate genes from galphimia spp. encoding enzymes of the galphimines triterpenoids synthesis pathway with anxiolytic and sedative effects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318123/ https://www.ncbi.nlm.nih.gov/pubmed/35890513 http://dx.doi.org/10.3390/plants11141879 |
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