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Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects

Galphimia spp. is popularly used in Mexican traditional medicine. Some populations of Galphimia exert anxiolytic and sedative effects due to the presence of the modified triterpenoids galphimines. However, the galphimine synthesis pathway has not yet been elucidated. Hence, in this study, a comparat...

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Autores principales: Iglesias, Dianella, de Donato Capote, Marcos, Méndez Tenorio, Alfonso, Valdivia, Ana Victoria, Gutiérrez-García, Claudia, Paul, Sujay, Iqbal, Hafiz M. N., Villarreal, María Luisa, Sharma, Ashutosh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318123/
https://www.ncbi.nlm.nih.gov/pubmed/35890513
http://dx.doi.org/10.3390/plants11141879
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author Iglesias, Dianella
de Donato Capote, Marcos
Méndez Tenorio, Alfonso
Valdivia, Ana Victoria
Gutiérrez-García, Claudia
Paul, Sujay
Iqbal, Hafiz M. N.
Villarreal, María Luisa
Sharma, Ashutosh
author_facet Iglesias, Dianella
de Donato Capote, Marcos
Méndez Tenorio, Alfonso
Valdivia, Ana Victoria
Gutiérrez-García, Claudia
Paul, Sujay
Iqbal, Hafiz M. N.
Villarreal, María Luisa
Sharma, Ashutosh
author_sort Iglesias, Dianella
collection PubMed
description Galphimia spp. is popularly used in Mexican traditional medicine. Some populations of Galphimia exert anxiolytic and sedative effects due to the presence of the modified triterpenoids galphimines. However, the galphimine synthesis pathway has not yet been elucidated. Hence, in this study, a comparative transcriptome analysis between two contrasting populations of Galphimia spp., a galphimine-producer, and a non-galphimine-producer, is performed using RNA-Seq in the Illumina Next Seq 550 platform to identify putative candidates genes that encode enzymes of this metabolic pathway. Transcriptome functional annotation was performed using the Blast2GO in levels of gene ontology. For differential expression analysis, edgeR, pheatmap, and Genie3 library were used. To validate transcriptome data, qPCR was conducted. In producer and non-producer plants of both populations of Galphimia spp., most of the transcripts were grouped in the Molecular Function level of gene ontology. A total of 680 differentially expressed transcripts between producer and non-producer plants were detected. In galphimine-producer plants, a larger number of highly expressed transcripts related to acyclic and polycyclic terpene synthesis were identified. As putative candidate genes involved in the galphimine synthesis pathway, P450 family members and enzymes with kinase activity were identified.
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spelling pubmed-93181232022-07-27 Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects Iglesias, Dianella de Donato Capote, Marcos Méndez Tenorio, Alfonso Valdivia, Ana Victoria Gutiérrez-García, Claudia Paul, Sujay Iqbal, Hafiz M. N. Villarreal, María Luisa Sharma, Ashutosh Plants (Basel) Article Galphimia spp. is popularly used in Mexican traditional medicine. Some populations of Galphimia exert anxiolytic and sedative effects due to the presence of the modified triterpenoids galphimines. However, the galphimine synthesis pathway has not yet been elucidated. Hence, in this study, a comparative transcriptome analysis between two contrasting populations of Galphimia spp., a galphimine-producer, and a non-galphimine-producer, is performed using RNA-Seq in the Illumina Next Seq 550 platform to identify putative candidates genes that encode enzymes of this metabolic pathway. Transcriptome functional annotation was performed using the Blast2GO in levels of gene ontology. For differential expression analysis, edgeR, pheatmap, and Genie3 library were used. To validate transcriptome data, qPCR was conducted. In producer and non-producer plants of both populations of Galphimia spp., most of the transcripts were grouped in the Molecular Function level of gene ontology. A total of 680 differentially expressed transcripts between producer and non-producer plants were detected. In galphimine-producer plants, a larger number of highly expressed transcripts related to acyclic and polycyclic terpene synthesis were identified. As putative candidate genes involved in the galphimine synthesis pathway, P450 family members and enzymes with kinase activity were identified. MDPI 2022-07-20 /pmc/articles/PMC9318123/ /pubmed/35890513 http://dx.doi.org/10.3390/plants11141879 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iglesias, Dianella
de Donato Capote, Marcos
Méndez Tenorio, Alfonso
Valdivia, Ana Victoria
Gutiérrez-García, Claudia
Paul, Sujay
Iqbal, Hafiz M. N.
Villarreal, María Luisa
Sharma, Ashutosh
Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
title Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
title_full Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
title_fullStr Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
title_full_unstemmed Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
title_short Identification of Putative Candidate Genes from Galphimia spp. Encoding Enzymes of the Galphimines Triterpenoids Synthesis Pathway with Anxiolytic and Sedative Effects
title_sort identification of putative candidate genes from galphimia spp. encoding enzymes of the galphimines triterpenoids synthesis pathway with anxiolytic and sedative effects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318123/
https://www.ncbi.nlm.nih.gov/pubmed/35890513
http://dx.doi.org/10.3390/plants11141879
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