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Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and compet...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318239/ https://www.ncbi.nlm.nih.gov/pubmed/35886898 http://dx.doi.org/10.3390/ijms23147552 |
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author | Valencia-Reséndiz, Diana Gabriela Villegas, Atenea Bahena, Daniel Palomino, Kenia Cornejo-Bravo, Jose Manuel Quintanar, Liliana Palomino-Vizcaino, Giovanni Alvarez-Salas, Luis Marat |
author_facet | Valencia-Reséndiz, Diana Gabriela Villegas, Atenea Bahena, Daniel Palomino, Kenia Cornejo-Bravo, Jose Manuel Quintanar, Liliana Palomino-Vizcaino, Giovanni Alvarez-Salas, Luis Marat |
author_sort | Valencia-Reséndiz, Diana Gabriela |
collection | PubMed |
description | The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and competition studies showed that color-shift inhibition is a consequence of direct nfGNP–VLP interaction and, thus, may produce a negative impact on the virus entry cell process. Here, an in vitro infection system based on the HPV16 pseudovirus (PsV) was used to stimulate the natural infection process in vitro. PsVs carry a pseudogenome with a reporter gene, resulting in a fluorescent signal when PsVs infect a cell, allowing quantification of the viral infection process. Aggregation assays showed that nfGNP-treated PsVs also inhibit color shift in the presence of NaCl. High-resolution microscopy confirmed nfGNP–PsV complex formation. In addition, PsVs can interact with silver nanoparticles, suggesting a generalized interaction of metallic nanoparticles with HPV16 capsids. The treatment of PsVs with nfGNPs produced viral infection inhibition at a higher level than heparin, the canonical inhibitor of HPV infection. Thus, nfGNPs can efficiently interfere with the HPV16 cell entry process and may represent a potential active component in prophylactic formulations to reduce the risk of HPV infection. |
format | Online Article Text |
id | pubmed-9318239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93182392022-07-27 Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection Valencia-Reséndiz, Diana Gabriela Villegas, Atenea Bahena, Daniel Palomino, Kenia Cornejo-Bravo, Jose Manuel Quintanar, Liliana Palomino-Vizcaino, Giovanni Alvarez-Salas, Luis Marat Int J Mol Sci Article The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and competition studies showed that color-shift inhibition is a consequence of direct nfGNP–VLP interaction and, thus, may produce a negative impact on the virus entry cell process. Here, an in vitro infection system based on the HPV16 pseudovirus (PsV) was used to stimulate the natural infection process in vitro. PsVs carry a pseudogenome with a reporter gene, resulting in a fluorescent signal when PsVs infect a cell, allowing quantification of the viral infection process. Aggregation assays showed that nfGNP-treated PsVs also inhibit color shift in the presence of NaCl. High-resolution microscopy confirmed nfGNP–PsV complex formation. In addition, PsVs can interact with silver nanoparticles, suggesting a generalized interaction of metallic nanoparticles with HPV16 capsids. The treatment of PsVs with nfGNPs produced viral infection inhibition at a higher level than heparin, the canonical inhibitor of HPV infection. Thus, nfGNPs can efficiently interfere with the HPV16 cell entry process and may represent a potential active component in prophylactic formulations to reduce the risk of HPV infection. MDPI 2022-07-07 /pmc/articles/PMC9318239/ /pubmed/35886898 http://dx.doi.org/10.3390/ijms23147552 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Valencia-Reséndiz, Diana Gabriela Villegas, Atenea Bahena, Daniel Palomino, Kenia Cornejo-Bravo, Jose Manuel Quintanar, Liliana Palomino-Vizcaino, Giovanni Alvarez-Salas, Luis Marat Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection |
title | Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection |
title_full | Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection |
title_fullStr | Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection |
title_full_unstemmed | Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection |
title_short | Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection |
title_sort | non-functionalized gold nanoparticles inhibit human papillomavirus (hpv) infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318239/ https://www.ncbi.nlm.nih.gov/pubmed/35886898 http://dx.doi.org/10.3390/ijms23147552 |
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