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Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection

The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and compet...

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Autores principales: Valencia-Reséndiz, Diana Gabriela, Villegas, Atenea, Bahena, Daniel, Palomino, Kenia, Cornejo-Bravo, Jose Manuel, Quintanar, Liliana, Palomino-Vizcaino, Giovanni, Alvarez-Salas, Luis Marat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318239/
https://www.ncbi.nlm.nih.gov/pubmed/35886898
http://dx.doi.org/10.3390/ijms23147552
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author Valencia-Reséndiz, Diana Gabriela
Villegas, Atenea
Bahena, Daniel
Palomino, Kenia
Cornejo-Bravo, Jose Manuel
Quintanar, Liliana
Palomino-Vizcaino, Giovanni
Alvarez-Salas, Luis Marat
author_facet Valencia-Reséndiz, Diana Gabriela
Villegas, Atenea
Bahena, Daniel
Palomino, Kenia
Cornejo-Bravo, Jose Manuel
Quintanar, Liliana
Palomino-Vizcaino, Giovanni
Alvarez-Salas, Luis Marat
author_sort Valencia-Reséndiz, Diana Gabriela
collection PubMed
description The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and competition studies showed that color-shift inhibition is a consequence of direct nfGNP–VLP interaction and, thus, may produce a negative impact on the virus entry cell process. Here, an in vitro infection system based on the HPV16 pseudovirus (PsV) was used to stimulate the natural infection process in vitro. PsVs carry a pseudogenome with a reporter gene, resulting in a fluorescent signal when PsVs infect a cell, allowing quantification of the viral infection process. Aggregation assays showed that nfGNP-treated PsVs also inhibit color shift in the presence of NaCl. High-resolution microscopy confirmed nfGNP–PsV complex formation. In addition, PsVs can interact with silver nanoparticles, suggesting a generalized interaction of metallic nanoparticles with HPV16 capsids. The treatment of PsVs with nfGNPs produced viral infection inhibition at a higher level than heparin, the canonical inhibitor of HPV infection. Thus, nfGNPs can efficiently interfere with the HPV16 cell entry process and may represent a potential active component in prophylactic formulations to reduce the risk of HPV infection.
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spelling pubmed-93182392022-07-27 Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection Valencia-Reséndiz, Diana Gabriela Villegas, Atenea Bahena, Daniel Palomino, Kenia Cornejo-Bravo, Jose Manuel Quintanar, Liliana Palomino-Vizcaino, Giovanni Alvarez-Salas, Luis Marat Int J Mol Sci Article The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and competition studies showed that color-shift inhibition is a consequence of direct nfGNP–VLP interaction and, thus, may produce a negative impact on the virus entry cell process. Here, an in vitro infection system based on the HPV16 pseudovirus (PsV) was used to stimulate the natural infection process in vitro. PsVs carry a pseudogenome with a reporter gene, resulting in a fluorescent signal when PsVs infect a cell, allowing quantification of the viral infection process. Aggregation assays showed that nfGNP-treated PsVs also inhibit color shift in the presence of NaCl. High-resolution microscopy confirmed nfGNP–PsV complex formation. In addition, PsVs can interact with silver nanoparticles, suggesting a generalized interaction of metallic nanoparticles with HPV16 capsids. The treatment of PsVs with nfGNPs produced viral infection inhibition at a higher level than heparin, the canonical inhibitor of HPV infection. Thus, nfGNPs can efficiently interfere with the HPV16 cell entry process and may represent a potential active component in prophylactic formulations to reduce the risk of HPV infection. MDPI 2022-07-07 /pmc/articles/PMC9318239/ /pubmed/35886898 http://dx.doi.org/10.3390/ijms23147552 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Valencia-Reséndiz, Diana Gabriela
Villegas, Atenea
Bahena, Daniel
Palomino, Kenia
Cornejo-Bravo, Jose Manuel
Quintanar, Liliana
Palomino-Vizcaino, Giovanni
Alvarez-Salas, Luis Marat
Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
title Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
title_full Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
title_fullStr Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
title_full_unstemmed Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
title_short Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection
title_sort non-functionalized gold nanoparticles inhibit human papillomavirus (hpv) infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318239/
https://www.ncbi.nlm.nih.gov/pubmed/35886898
http://dx.doi.org/10.3390/ijms23147552
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