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Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients

Multiple myeloma results from the clonal proliferation of abnormal plasma cells (PCs) in the bone marrow (BM). In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome...

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Autores principales: Dragoș, Mihaiela L., Ivanov, Iuliu C., Mențel, Mihaela, Văcărean-Trandafir, Irina C., Sireteanu, Adriana, Titianu, Amalia A., Dăscălescu, Angela S., Stache, Alexandru B., Jitaru, Daniela, Gorgan, Dragoș L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318311/
https://www.ncbi.nlm.nih.gov/pubmed/35886877
http://dx.doi.org/10.3390/ijms23147530
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author Dragoș, Mihaiela L.
Ivanov, Iuliu C.
Mențel, Mihaela
Văcărean-Trandafir, Irina C.
Sireteanu, Adriana
Titianu, Amalia A.
Dăscălescu, Angela S.
Stache, Alexandru B.
Jitaru, Daniela
Gorgan, Dragoș L.
author_facet Dragoș, Mihaiela L.
Ivanov, Iuliu C.
Mențel, Mihaela
Văcărean-Trandafir, Irina C.
Sireteanu, Adriana
Titianu, Amalia A.
Dăscălescu, Angela S.
Stache, Alexandru B.
Jitaru, Daniela
Gorgan, Dragoș L.
author_sort Dragoș, Mihaiela L.
collection PubMed
description Multiple myeloma results from the clonal proliferation of abnormal plasma cells (PCs) in the bone marrow (BM). In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome (detected by multiplex ligation-dependent probe amplification (MLPA)) to assess their impact on prognosis in newly diagnosed MM patients. Statistically significant results were obtained when different stages of PC maturation (classified based on CD19 and CD81 expression) were associated with CD117 expression and identified CNAs. In the intermediately differentiated PC group (CD19(−) CD81(+)), patients who didn’t express CD117 had a lower median progression free survival (PFS) (p = 0.024). Moreover, within this group, patients with less than three adverse CNAs, which harbor CD117, had a better outcome with a PFS of more than 48 months compared with 19 months (p = 0.008). Considering all the results, our study suggested the need to integrate both the CD markers and copy number alterations to evaluate the prognosis of newly diagnosed multiple myeloma patients.
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spelling pubmed-93183112022-07-27 Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients Dragoș, Mihaiela L. Ivanov, Iuliu C. Mențel, Mihaela Văcărean-Trandafir, Irina C. Sireteanu, Adriana Titianu, Amalia A. Dăscălescu, Angela S. Stache, Alexandru B. Jitaru, Daniela Gorgan, Dragoș L. Int J Mol Sci Article Multiple myeloma results from the clonal proliferation of abnormal plasma cells (PCs) in the bone marrow (BM). In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome (detected by multiplex ligation-dependent probe amplification (MLPA)) to assess their impact on prognosis in newly diagnosed MM patients. Statistically significant results were obtained when different stages of PC maturation (classified based on CD19 and CD81 expression) were associated with CD117 expression and identified CNAs. In the intermediately differentiated PC group (CD19(−) CD81(+)), patients who didn’t express CD117 had a lower median progression free survival (PFS) (p = 0.024). Moreover, within this group, patients with less than three adverse CNAs, which harbor CD117, had a better outcome with a PFS of more than 48 months compared with 19 months (p = 0.008). Considering all the results, our study suggested the need to integrate both the CD markers and copy number alterations to evaluate the prognosis of newly diagnosed multiple myeloma patients. MDPI 2022-07-07 /pmc/articles/PMC9318311/ /pubmed/35886877 http://dx.doi.org/10.3390/ijms23147530 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dragoș, Mihaiela L.
Ivanov, Iuliu C.
Mențel, Mihaela
Văcărean-Trandafir, Irina C.
Sireteanu, Adriana
Titianu, Amalia A.
Dăscălescu, Angela S.
Stache, Alexandru B.
Jitaru, Daniela
Gorgan, Dragoș L.
Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
title Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
title_full Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
title_fullStr Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
title_full_unstemmed Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
title_short Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
title_sort prognostic value of association of copy number alterations and cell-surface expression markers in newly diagnosed multiple myeloma patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318311/
https://www.ncbi.nlm.nih.gov/pubmed/35886877
http://dx.doi.org/10.3390/ijms23147530
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