Cargando…

T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs

Antibodies in response to antigens are related to the immune repertoire of T- and B-cell receptors. However, some patients with chronic hepatitis B (CHB) have coexisting HBsAg and anti-HBsAg antibodies (anti-HBs) that cannot neutralize HBV. We attempted to investigate the repertoires that produce th...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhan, Qiao, Chang, Le, Wu, Jian, Zhang, Zhiyuan, Xu, Jinghang, Yu, Yanyan, Feng, Zhenru, Zeng, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318409/
https://www.ncbi.nlm.nih.gov/pubmed/35889974
http://dx.doi.org/10.3390/pathogens11070727
_version_ 1784755283608731648
author Zhan, Qiao
Chang, Le
Wu, Jian
Zhang, Zhiyuan
Xu, Jinghang
Yu, Yanyan
Feng, Zhenru
Zeng, Zheng
author_facet Zhan, Qiao
Chang, Le
Wu, Jian
Zhang, Zhiyuan
Xu, Jinghang
Yu, Yanyan
Feng, Zhenru
Zeng, Zheng
author_sort Zhan, Qiao
collection PubMed
description Antibodies in response to antigens are related to the immune repertoire of T- and B-cell receptors. However, some patients with chronic hepatitis B (CHB) have coexisting HBsAg and anti-HBsAg antibodies (anti-HBs) that cannot neutralize HBV. We attempted to investigate the repertoires that produce this response in CHB patients. The T-cell receptor β chain (TRB) and B-cell receptor (BCR) repertoires of peripheral blood genomic DNA were analyzed using MiXCR. T-cell receptor (TCR) cluster analysis was carried out by clusTCR, and motifs prediction was selected by Multiple Em for Motif Elicitation (MEME). A total of 76 subjects were enrolled, including 26 HBsAg and anti-HBs coexisting patients with CHB (DP group), 25 anti-HBs single-positive healthy people (SP group), and 25 CHB patients (CHB group). The clone length of BCR in 39, 90 was significantly different among these groups (p = 0.005, 0.036). The motif “CASSLG” in the DP group was significantly higher than SP and CHB groups and may relate to coexistence, and the motif “GAGPLT” was only shown in the SP group and may relate to anti-HB expression. These provide important insights into vaccine development and CHB treatment.
format Online
Article
Text
id pubmed-9318409
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93184092022-07-27 T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs Zhan, Qiao Chang, Le Wu, Jian Zhang, Zhiyuan Xu, Jinghang Yu, Yanyan Feng, Zhenru Zeng, Zheng Pathogens Article Antibodies in response to antigens are related to the immune repertoire of T- and B-cell receptors. However, some patients with chronic hepatitis B (CHB) have coexisting HBsAg and anti-HBsAg antibodies (anti-HBs) that cannot neutralize HBV. We attempted to investigate the repertoires that produce this response in CHB patients. The T-cell receptor β chain (TRB) and B-cell receptor (BCR) repertoires of peripheral blood genomic DNA were analyzed using MiXCR. T-cell receptor (TCR) cluster analysis was carried out by clusTCR, and motifs prediction was selected by Multiple Em for Motif Elicitation (MEME). A total of 76 subjects were enrolled, including 26 HBsAg and anti-HBs coexisting patients with CHB (DP group), 25 anti-HBs single-positive healthy people (SP group), and 25 CHB patients (CHB group). The clone length of BCR in 39, 90 was significantly different among these groups (p = 0.005, 0.036). The motif “CASSLG” in the DP group was significantly higher than SP and CHB groups and may relate to coexistence, and the motif “GAGPLT” was only shown in the SP group and may relate to anti-HB expression. These provide important insights into vaccine development and CHB treatment. MDPI 2022-06-26 /pmc/articles/PMC9318409/ /pubmed/35889974 http://dx.doi.org/10.3390/pathogens11070727 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhan, Qiao
Chang, Le
Wu, Jian
Zhang, Zhiyuan
Xu, Jinghang
Yu, Yanyan
Feng, Zhenru
Zeng, Zheng
T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs
title T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs
title_full T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs
title_fullStr T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs
title_full_unstemmed T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs
title_short T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs
title_sort t-cell receptor β chain and b-cell receptor repertoires in chronic hepatitis b patients with coexisting hbsag and anti-hbs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318409/
https://www.ncbi.nlm.nih.gov/pubmed/35889974
http://dx.doi.org/10.3390/pathogens11070727
work_keys_str_mv AT zhanqiao tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT changle tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT wujian tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT zhangzhiyuan tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT xujinghang tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT yuyanyan tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT fengzhenru tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs
AT zengzheng tcellreceptorbchainandbcellreceptorrepertoiresinchronichepatitisbpatientswithcoexistinghbsagandantihbs