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GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)

Soybeans are a major crop that produce the best vegetable oil and protein for use in food and beverage products worldwide. However, one of the most well-known viral infections affecting soybeans is the Soybean Mosaic Virus (SMV), a member of the Potyviridae family. A crucial method for preventing SM...

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Autores principales: Song, Shuang, Wang, Jing, Yang, Xingqi, Zhang, Xuan, Xin, Xiuli, Liu, Chunyan, Zou, Jianan, Cheng, Xiaofei, Zhang, Ning, Hu, Yuxi, Wang, Jinhui, Chen, Qingshan, Xin, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318458/
https://www.ncbi.nlm.nih.gov/pubmed/35886929
http://dx.doi.org/10.3390/ijms23147577
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author Song, Shuang
Wang, Jing
Yang, Xingqi
Zhang, Xuan
Xin, Xiuli
Liu, Chunyan
Zou, Jianan
Cheng, Xiaofei
Zhang, Ning
Hu, Yuxi
Wang, Jinhui
Chen, Qingshan
Xin, Dawei
author_facet Song, Shuang
Wang, Jing
Yang, Xingqi
Zhang, Xuan
Xin, Xiuli
Liu, Chunyan
Zou, Jianan
Cheng, Xiaofei
Zhang, Ning
Hu, Yuxi
Wang, Jinhui
Chen, Qingshan
Xin, Dawei
author_sort Song, Shuang
collection PubMed
description Soybeans are a major crop that produce the best vegetable oil and protein for use in food and beverage products worldwide. However, one of the most well-known viral infections affecting soybeans is the Soybean Mosaic Virus (SMV), a member of the Potyviridae family. A crucial method for preventing SMV damage is the breeding of resistant soybean cultivars. Adult resistance and resistance of seedcoat mottling are two types of resistance to SMV. Most studies have focused on adult-plant resistance but not on the resistance to seedcoat mottling. In this study, chromosome segment-substituted lines derived from a cross between Suinong14 (cultivated soybean) and ZYD00006 (wild soybean) were used to identify the chromosome region and candidate genes underlying soybean resistance to seed coat mottling. Herein, two quantitative trait loci (QTLs) were found on chromosome 17, and eighteen genes were found in the QTL region. RNA-seq was used to evaluate the differentially expressed genes (DEGs) among the eighteen genes located in the QTLs. According to the obtained data, variations were observed in the expression of five genes following SMV infection. Furthermore, Nicotiana benthamiana was subjected to an Agrobacterium-mediated transient expression assay to investigate the role of the five candidate genes in SMV resistance. It has also been revealed that Glyma.17g238900 encoding a RICE SALT SENSITIVE 3-like protein (RSS3L) can inhibit the multiplication of SMV in N. benthamiana. Moreover, two nonsynonymous single-nucleotide polymorphisms (SNPs) were found in the coding sequence of Glyma.17g238900 derived from the wild soybean ZYD00006 (GsRSS3L), and the two amino acid mutants may be associated with SMV resistance. Hence, it has been suggested that GsRSS3L confers seedcoat mottling resistance, shedding light on the mechanism of soybean resistance to SMV.
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spelling pubmed-93184582022-07-27 GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc) Song, Shuang Wang, Jing Yang, Xingqi Zhang, Xuan Xin, Xiuli Liu, Chunyan Zou, Jianan Cheng, Xiaofei Zhang, Ning Hu, Yuxi Wang, Jinhui Chen, Qingshan Xin, Dawei Int J Mol Sci Article Soybeans are a major crop that produce the best vegetable oil and protein for use in food and beverage products worldwide. However, one of the most well-known viral infections affecting soybeans is the Soybean Mosaic Virus (SMV), a member of the Potyviridae family. A crucial method for preventing SMV damage is the breeding of resistant soybean cultivars. Adult resistance and resistance of seedcoat mottling are two types of resistance to SMV. Most studies have focused on adult-plant resistance but not on the resistance to seedcoat mottling. In this study, chromosome segment-substituted lines derived from a cross between Suinong14 (cultivated soybean) and ZYD00006 (wild soybean) were used to identify the chromosome region and candidate genes underlying soybean resistance to seed coat mottling. Herein, two quantitative trait loci (QTLs) were found on chromosome 17, and eighteen genes were found in the QTL region. RNA-seq was used to evaluate the differentially expressed genes (DEGs) among the eighteen genes located in the QTLs. According to the obtained data, variations were observed in the expression of five genes following SMV infection. Furthermore, Nicotiana benthamiana was subjected to an Agrobacterium-mediated transient expression assay to investigate the role of the five candidate genes in SMV resistance. It has also been revealed that Glyma.17g238900 encoding a RICE SALT SENSITIVE 3-like protein (RSS3L) can inhibit the multiplication of SMV in N. benthamiana. Moreover, two nonsynonymous single-nucleotide polymorphisms (SNPs) were found in the coding sequence of Glyma.17g238900 derived from the wild soybean ZYD00006 (GsRSS3L), and the two amino acid mutants may be associated with SMV resistance. Hence, it has been suggested that GsRSS3L confers seedcoat mottling resistance, shedding light on the mechanism of soybean resistance to SMV. MDPI 2022-07-08 /pmc/articles/PMC9318458/ /pubmed/35886929 http://dx.doi.org/10.3390/ijms23147577 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Shuang
Wang, Jing
Yang, Xingqi
Zhang, Xuan
Xin, Xiuli
Liu, Chunyan
Zou, Jianan
Cheng, Xiaofei
Zhang, Ning
Hu, Yuxi
Wang, Jinhui
Chen, Qingshan
Xin, Dawei
GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)
title GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)
title_full GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)
title_fullStr GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)
title_full_unstemmed GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)
title_short GsRSS3L, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (Glycine soja Sieb. and Zucc)
title_sort gsrss3l, a candidate gene underlying soybean resistance to seedcoat mottling derived from wild soybean (glycine soja sieb. and zucc)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318458/
https://www.ncbi.nlm.nih.gov/pubmed/35886929
http://dx.doi.org/10.3390/ijms23147577
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