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Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer

The solid self-nanoemulsifying drug delivery system (s-SNEDDS) is a growing platform for the delivery of drugs via oral route. In the present work, tamoxifen (TAM) was loaded in SNEDDS with resveratrol (RES), which is a potent chemotherapeutic, antioxidant, anti-inflammatory and P-gp inhibitor for e...

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Autores principales: Shrivastava, Nupur, Parikh, Ankit, Dewangan, Rikeshwer Prasad, Biswas, Largee, Verma, Anita Kamra, Mittal, Saurabh, Ali, Javed, Garg, Sanjay, Baboota, Sanjula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318459/
https://www.ncbi.nlm.nih.gov/pubmed/35890384
http://dx.doi.org/10.3390/pharmaceutics14071486
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author Shrivastava, Nupur
Parikh, Ankit
Dewangan, Rikeshwer Prasad
Biswas, Largee
Verma, Anita Kamra
Mittal, Saurabh
Ali, Javed
Garg, Sanjay
Baboota, Sanjula
author_facet Shrivastava, Nupur
Parikh, Ankit
Dewangan, Rikeshwer Prasad
Biswas, Largee
Verma, Anita Kamra
Mittal, Saurabh
Ali, Javed
Garg, Sanjay
Baboota, Sanjula
author_sort Shrivastava, Nupur
collection PubMed
description The solid self-nanoemulsifying drug delivery system (s-SNEDDS) is a growing platform for the delivery of drugs via oral route. In the present work, tamoxifen (TAM) was loaded in SNEDDS with resveratrol (RES), which is a potent chemotherapeutic, antioxidant, anti-inflammatory and P-gp inhibitor for enhancing bioavailability and to obtain synergistic anti-cancer effect against breast cancer. SNEDDS were developed using capmul MCM as oil, Tween 80 as surfactant and transcutol-HP as co-surfactant and optimized by central composite rotatable design. Neusilin US2 concentration was optimized for adsorption of liquid SNEDDS to prepare s-SNEDDS. The developed formulation was characterized and investigated for various in vitro and cell line comparative studies. Optimized TAM-RES-s-SNEDDS showed spherical droplets of a size less than 200 nm. In all in vitro studies, TAM-RES-s-SNEDDS showed significantly improved (p ˂ 0.05) release and permeation across the dialysis membrane and intestinal lumen. Moreover, TAM-RES-s-SNEDDS possessed significantly greater therapeutic efficacy (p < 0.05) and better internalization on the MCF-7 cell line as compared to the conventional formulation. Additionally, oral bioavailability of TAM from SNEDDS was 1.63 folds significantly higher (p < 0.05) than that of combination suspension and 4.16 folds significantly higher (p < 0.05) than TAM suspension. Thus, findings suggest that TAM- RES-s-SNEDDS can be the future delivery system that potentially delivers both drugs to cancer cells for better treatment.
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spelling pubmed-93184592022-07-27 Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer Shrivastava, Nupur Parikh, Ankit Dewangan, Rikeshwer Prasad Biswas, Largee Verma, Anita Kamra Mittal, Saurabh Ali, Javed Garg, Sanjay Baboota, Sanjula Pharmaceutics Article The solid self-nanoemulsifying drug delivery system (s-SNEDDS) is a growing platform for the delivery of drugs via oral route. In the present work, tamoxifen (TAM) was loaded in SNEDDS with resveratrol (RES), which is a potent chemotherapeutic, antioxidant, anti-inflammatory and P-gp inhibitor for enhancing bioavailability and to obtain synergistic anti-cancer effect against breast cancer. SNEDDS were developed using capmul MCM as oil, Tween 80 as surfactant and transcutol-HP as co-surfactant and optimized by central composite rotatable design. Neusilin US2 concentration was optimized for adsorption of liquid SNEDDS to prepare s-SNEDDS. The developed formulation was characterized and investigated for various in vitro and cell line comparative studies. Optimized TAM-RES-s-SNEDDS showed spherical droplets of a size less than 200 nm. In all in vitro studies, TAM-RES-s-SNEDDS showed significantly improved (p ˂ 0.05) release and permeation across the dialysis membrane and intestinal lumen. Moreover, TAM-RES-s-SNEDDS possessed significantly greater therapeutic efficacy (p < 0.05) and better internalization on the MCF-7 cell line as compared to the conventional formulation. Additionally, oral bioavailability of TAM from SNEDDS was 1.63 folds significantly higher (p < 0.05) than that of combination suspension and 4.16 folds significantly higher (p < 0.05) than TAM suspension. Thus, findings suggest that TAM- RES-s-SNEDDS can be the future delivery system that potentially delivers both drugs to cancer cells for better treatment. MDPI 2022-07-18 /pmc/articles/PMC9318459/ /pubmed/35890384 http://dx.doi.org/10.3390/pharmaceutics14071486 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shrivastava, Nupur
Parikh, Ankit
Dewangan, Rikeshwer Prasad
Biswas, Largee
Verma, Anita Kamra
Mittal, Saurabh
Ali, Javed
Garg, Sanjay
Baboota, Sanjula
Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer
title Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer
title_full Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer
title_fullStr Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer
title_full_unstemmed Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer
title_short Solid Self-Nano Emulsifying Nanoplatform Loaded with Tamoxifen and Resveratrol for Treatment of Breast Cancer
title_sort solid self-nano emulsifying nanoplatform loaded with tamoxifen and resveratrol for treatment of breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318459/
https://www.ncbi.nlm.nih.gov/pubmed/35890384
http://dx.doi.org/10.3390/pharmaceutics14071486
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