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Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton

China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding proc...

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Autores principales: Zheng, Juyun, Zhang, Zeliang, Liang, Yajun, Gong, Zhaolong, Zhang, Nala, Ditta, Allah, Sang, Zhiwei, Wang, Junduo, Li, Xueyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318479/
https://www.ncbi.nlm.nih.gov/pubmed/35885942
http://dx.doi.org/10.3390/genes13071159
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author Zheng, Juyun
Zhang, Zeliang
Liang, Yajun
Gong, Zhaolong
Zhang, Nala
Ditta, Allah
Sang, Zhiwei
Wang, Junduo
Li, Xueyuan
author_facet Zheng, Juyun
Zhang, Zeliang
Liang, Yajun
Gong, Zhaolong
Zhang, Nala
Ditta, Allah
Sang, Zhiwei
Wang, Junduo
Li, Xueyuan
author_sort Zheng, Juyun
collection PubMed
description China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding procedures are therefore critical to the cotton industry’s success. The drought-resistant cotton variety Xinluzhong No. 82 and the drought-sensitive cotton variety Kexin No. 1 were utilised in this study to uncover a batch of drought-resistant candidate genes using whole transcriptome sequencing. The following are the key research findings: A competing endogenous RNA network (ceRNA) was built using complete transcriptional sequencing to screen the core genes in the core pathway, and two drought-related candidate genes were discovered. It was found that γ-aminobutyric acid aminotransferase (GhGABA-T, Gohir.A11G156000) was upregulated at 0 h vs. 12 h and downregulated at 12 h vs. 24 h. L-Aspartate oxidase (GhAO, Gohir.A07G220600) was downregulated at 0 h vs. 12 h and upregulated at 12 h vs. 24 h. GABA-T is analogous to a pyridoxal phosphate-dependent transferase superfamily protein (POP2) in Arabidopsis thaliana and influences plant drought resistance by controlling γ-aminobutyric acid (GABA) concentration. The analogue of GhAO in A. thaliana is involved in the early steps of nicotinamide adenine dinucleotide (NAD) production as well as in plant antioxidant responses. This study revealed that gene expression regulatory networks can be used for rapid screening of reliable drought resistance genes and then utilised to validate gene function.
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spelling pubmed-93184792022-07-27 Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton Zheng, Juyun Zhang, Zeliang Liang, Yajun Gong, Zhaolong Zhang, Nala Ditta, Allah Sang, Zhiwei Wang, Junduo Li, Xueyuan Genes (Basel) Article China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding procedures are therefore critical to the cotton industry’s success. The drought-resistant cotton variety Xinluzhong No. 82 and the drought-sensitive cotton variety Kexin No. 1 were utilised in this study to uncover a batch of drought-resistant candidate genes using whole transcriptome sequencing. The following are the key research findings: A competing endogenous RNA network (ceRNA) was built using complete transcriptional sequencing to screen the core genes in the core pathway, and two drought-related candidate genes were discovered. It was found that γ-aminobutyric acid aminotransferase (GhGABA-T, Gohir.A11G156000) was upregulated at 0 h vs. 12 h and downregulated at 12 h vs. 24 h. L-Aspartate oxidase (GhAO, Gohir.A07G220600) was downregulated at 0 h vs. 12 h and upregulated at 12 h vs. 24 h. GABA-T is analogous to a pyridoxal phosphate-dependent transferase superfamily protein (POP2) in Arabidopsis thaliana and influences plant drought resistance by controlling γ-aminobutyric acid (GABA) concentration. The analogue of GhAO in A. thaliana is involved in the early steps of nicotinamide adenine dinucleotide (NAD) production as well as in plant antioxidant responses. This study revealed that gene expression regulatory networks can be used for rapid screening of reliable drought resistance genes and then utilised to validate gene function. MDPI 2022-06-27 /pmc/articles/PMC9318479/ /pubmed/35885942 http://dx.doi.org/10.3390/genes13071159 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Juyun
Zhang, Zeliang
Liang, Yajun
Gong, Zhaolong
Zhang, Nala
Ditta, Allah
Sang, Zhiwei
Wang, Junduo
Li, Xueyuan
Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
title Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
title_full Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
title_fullStr Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
title_full_unstemmed Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
title_short Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
title_sort whole transcriptome sequencing reveals drought resistance-related genes in upland cotton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318479/
https://www.ncbi.nlm.nih.gov/pubmed/35885942
http://dx.doi.org/10.3390/genes13071159
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