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Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton
China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding proc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318479/ https://www.ncbi.nlm.nih.gov/pubmed/35885942 http://dx.doi.org/10.3390/genes13071159 |
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author | Zheng, Juyun Zhang, Zeliang Liang, Yajun Gong, Zhaolong Zhang, Nala Ditta, Allah Sang, Zhiwei Wang, Junduo Li, Xueyuan |
author_facet | Zheng, Juyun Zhang, Zeliang Liang, Yajun Gong, Zhaolong Zhang, Nala Ditta, Allah Sang, Zhiwei Wang, Junduo Li, Xueyuan |
author_sort | Zheng, Juyun |
collection | PubMed |
description | China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding procedures are therefore critical to the cotton industry’s success. The drought-resistant cotton variety Xinluzhong No. 82 and the drought-sensitive cotton variety Kexin No. 1 were utilised in this study to uncover a batch of drought-resistant candidate genes using whole transcriptome sequencing. The following are the key research findings: A competing endogenous RNA network (ceRNA) was built using complete transcriptional sequencing to screen the core genes in the core pathway, and two drought-related candidate genes were discovered. It was found that γ-aminobutyric acid aminotransferase (GhGABA-T, Gohir.A11G156000) was upregulated at 0 h vs. 12 h and downregulated at 12 h vs. 24 h. L-Aspartate oxidase (GhAO, Gohir.A07G220600) was downregulated at 0 h vs. 12 h and upregulated at 12 h vs. 24 h. GABA-T is analogous to a pyridoxal phosphate-dependent transferase superfamily protein (POP2) in Arabidopsis thaliana and influences plant drought resistance by controlling γ-aminobutyric acid (GABA) concentration. The analogue of GhAO in A. thaliana is involved in the early steps of nicotinamide adenine dinucleotide (NAD) production as well as in plant antioxidant responses. This study revealed that gene expression regulatory networks can be used for rapid screening of reliable drought resistance genes and then utilised to validate gene function. |
format | Online Article Text |
id | pubmed-9318479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93184792022-07-27 Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton Zheng, Juyun Zhang, Zeliang Liang, Yajun Gong, Zhaolong Zhang, Nala Ditta, Allah Sang, Zhiwei Wang, Junduo Li, Xueyuan Genes (Basel) Article China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding procedures are therefore critical to the cotton industry’s success. The drought-resistant cotton variety Xinluzhong No. 82 and the drought-sensitive cotton variety Kexin No. 1 were utilised in this study to uncover a batch of drought-resistant candidate genes using whole transcriptome sequencing. The following are the key research findings: A competing endogenous RNA network (ceRNA) was built using complete transcriptional sequencing to screen the core genes in the core pathway, and two drought-related candidate genes were discovered. It was found that γ-aminobutyric acid aminotransferase (GhGABA-T, Gohir.A11G156000) was upregulated at 0 h vs. 12 h and downregulated at 12 h vs. 24 h. L-Aspartate oxidase (GhAO, Gohir.A07G220600) was downregulated at 0 h vs. 12 h and upregulated at 12 h vs. 24 h. GABA-T is analogous to a pyridoxal phosphate-dependent transferase superfamily protein (POP2) in Arabidopsis thaliana and influences plant drought resistance by controlling γ-aminobutyric acid (GABA) concentration. The analogue of GhAO in A. thaliana is involved in the early steps of nicotinamide adenine dinucleotide (NAD) production as well as in plant antioxidant responses. This study revealed that gene expression regulatory networks can be used for rapid screening of reliable drought resistance genes and then utilised to validate gene function. MDPI 2022-06-27 /pmc/articles/PMC9318479/ /pubmed/35885942 http://dx.doi.org/10.3390/genes13071159 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zheng, Juyun Zhang, Zeliang Liang, Yajun Gong, Zhaolong Zhang, Nala Ditta, Allah Sang, Zhiwei Wang, Junduo Li, Xueyuan Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton |
title | Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton |
title_full | Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton |
title_fullStr | Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton |
title_full_unstemmed | Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton |
title_short | Whole Transcriptome Sequencing Reveals Drought Resistance-Related Genes in Upland Cotton |
title_sort | whole transcriptome sequencing reveals drought resistance-related genes in upland cotton |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318479/ https://www.ncbi.nlm.nih.gov/pubmed/35885942 http://dx.doi.org/10.3390/genes13071159 |
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