Cargando…

Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin

In highly aggressive tumors, cancer cells may form channel-like structures through a process known as vasculogenic mimicry (VM). VM is generally associated with metastasis, mesenchymal phenotype, and treatment resistance. VM can be driven by antiangiogenic treatments and/or tumor microenvironment-de...

Descripción completa

Detalles Bibliográficos
Autores principales: Morales-Guadarrama, Gabriela, Méndez-Pérez, Edgar A., García-Quiroz, Janice, Avila, Euclides, García-Becerra, Rocío, Zentella-Dehesa, Alejandro, Larrea, Fernando, Díaz, Lorenza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318499/
https://www.ncbi.nlm.nih.gov/pubmed/35887002
http://dx.doi.org/10.3390/ijms23147659
_version_ 1784755306713055232
author Morales-Guadarrama, Gabriela
Méndez-Pérez, Edgar A.
García-Quiroz, Janice
Avila, Euclides
García-Becerra, Rocío
Zentella-Dehesa, Alejandro
Larrea, Fernando
Díaz, Lorenza
author_facet Morales-Guadarrama, Gabriela
Méndez-Pérez, Edgar A.
García-Quiroz, Janice
Avila, Euclides
García-Becerra, Rocío
Zentella-Dehesa, Alejandro
Larrea, Fernando
Díaz, Lorenza
author_sort Morales-Guadarrama, Gabriela
collection PubMed
description In highly aggressive tumors, cancer cells may form channel-like structures through a process known as vasculogenic mimicry (VM). VM is generally associated with metastasis, mesenchymal phenotype, and treatment resistance. VM can be driven by antiangiogenic treatments and/or tumor microenvironment-derived factors, including those from the endothelium. Curcumin, a turmeric product, inhibits VM in some tumors, while calcitriol, the most active vitamin D metabolite, exerts potent antineoplastic effects. However, the effect of these natural products on VM in breast cancer remains unknown. Herein, we studied the effect of both compounds on triple-negative breast cancer (TNBC) VM-capacity in a co-culture model. The process of endothelial cell-induced VM in two human TNBC cell lines was robustly inhibited by calcitriol and partially by curcumin. Calcitriol promoted TNBC cells’ morphological change from spindle-like to cobblestone-shape, while curcumin diminished VM 3D-structure. Notably, the treatments dephosphorylated several active kinases, especially those involved in the PI3K/Akt pathway. In summary, calcitriol and curcumin disrupted endothelium-induced VM in TNBC cells partially by PI3K/Akt inactivation and mesenchymal phenotype inhibition. Our results support the possible use of these natural compounds as adjuvants for VM inactivation in patients with malignant tumors inherently capable of forming VM, or those with antiangiogenic therapy, warranting further in vivo studies.
format Online
Article
Text
id pubmed-9318499
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93184992022-07-27 Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin Morales-Guadarrama, Gabriela Méndez-Pérez, Edgar A. García-Quiroz, Janice Avila, Euclides García-Becerra, Rocío Zentella-Dehesa, Alejandro Larrea, Fernando Díaz, Lorenza Int J Mol Sci Article In highly aggressive tumors, cancer cells may form channel-like structures through a process known as vasculogenic mimicry (VM). VM is generally associated with metastasis, mesenchymal phenotype, and treatment resistance. VM can be driven by antiangiogenic treatments and/or tumor microenvironment-derived factors, including those from the endothelium. Curcumin, a turmeric product, inhibits VM in some tumors, while calcitriol, the most active vitamin D metabolite, exerts potent antineoplastic effects. However, the effect of these natural products on VM in breast cancer remains unknown. Herein, we studied the effect of both compounds on triple-negative breast cancer (TNBC) VM-capacity in a co-culture model. The process of endothelial cell-induced VM in two human TNBC cell lines was robustly inhibited by calcitriol and partially by curcumin. Calcitriol promoted TNBC cells’ morphological change from spindle-like to cobblestone-shape, while curcumin diminished VM 3D-structure. Notably, the treatments dephosphorylated several active kinases, especially those involved in the PI3K/Akt pathway. In summary, calcitriol and curcumin disrupted endothelium-induced VM in TNBC cells partially by PI3K/Akt inactivation and mesenchymal phenotype inhibition. Our results support the possible use of these natural compounds as adjuvants for VM inactivation in patients with malignant tumors inherently capable of forming VM, or those with antiangiogenic therapy, warranting further in vivo studies. MDPI 2022-07-11 /pmc/articles/PMC9318499/ /pubmed/35887002 http://dx.doi.org/10.3390/ijms23147659 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morales-Guadarrama, Gabriela
Méndez-Pérez, Edgar A.
García-Quiroz, Janice
Avila, Euclides
García-Becerra, Rocío
Zentella-Dehesa, Alejandro
Larrea, Fernando
Díaz, Lorenza
Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin
title Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin
title_full Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin
title_fullStr Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin
title_full_unstemmed Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin
title_short Endothelium-Dependent Induction of Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cells Is Inhibited by Calcitriol and Curcumin
title_sort endothelium-dependent induction of vasculogenic mimicry in human triple-negative breast cancer cells is inhibited by calcitriol and curcumin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318499/
https://www.ncbi.nlm.nih.gov/pubmed/35887002
http://dx.doi.org/10.3390/ijms23147659
work_keys_str_mv AT moralesguadarramagabriela endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT mendezperezedgara endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT garciaquirozjanice endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT avilaeuclides endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT garciabecerrarocio endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT zentelladehesaalejandro endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT larreafernando endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin
AT diazlorenza endotheliumdependentinductionofvasculogenicmimicryinhumantriplenegativebreastcancercellsisinhibitedbycalcitriolandcurcumin