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Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study
Obesity is a risk factor of chronic kidney disease (CKD), leading to alterations in the renal vascular structure. This study tested if renal vascular density and tortuosity was quantifiable in vivo in obese rats using microbubble-based super-resolution ultrasound imaging. The kidneys of two 11-week-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318608/ https://www.ncbi.nlm.nih.gov/pubmed/35885531 http://dx.doi.org/10.3390/diagnostics12071626 |
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author | Søgaard, Stinne Byrholdt Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Christoffersen, Christina Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin |
author_facet | Søgaard, Stinne Byrholdt Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Christoffersen, Christina Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin |
author_sort | Søgaard, Stinne Byrholdt |
collection | PubMed |
description | Obesity is a risk factor of chronic kidney disease (CKD), leading to alterations in the renal vascular structure. This study tested if renal vascular density and tortuosity was quantifiable in vivo in obese rats using microbubble-based super-resolution ultrasound imaging. The kidneys of two 11-week-old and two 20-week-old male obese Zucker rats were compared with age-matched male lean Zucker rats. The super-resolution ultrasound images were manually divided into inner medulla, outer medulla, and cortex, and each area was subdivided into arteries and veins. We quantified vascular density and tortuosity, number of detected microbubbles, and generated tracks. For comparison, we assessed glomerular filtration rate, albumin/creatinine ratio, and renal histology to evaluate CKD. The number of detected microbubbles and generated tracks varied between animals and significantly affected quantification of vessel density. In areas with a comparable number of tracks, density increased in the obese animals, concomitant with a decrease in glomerular filtration rate and an increase in albumin/creatinine ratio, but without any pathology in the histological staining. The results indicate that super-resolution ultrasound imaging can be used to quantify structural alterations in the renal vasculature. Techniques to generate more comparable number of microbubble tracks and confirmation of the findings in larger-scale studies are needed. |
format | Online Article Text |
id | pubmed-9318608 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93186082022-07-27 Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study Søgaard, Stinne Byrholdt Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Christoffersen, Christina Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin Diagnostics (Basel) Article Obesity is a risk factor of chronic kidney disease (CKD), leading to alterations in the renal vascular structure. This study tested if renal vascular density and tortuosity was quantifiable in vivo in obese rats using microbubble-based super-resolution ultrasound imaging. The kidneys of two 11-week-old and two 20-week-old male obese Zucker rats were compared with age-matched male lean Zucker rats. The super-resolution ultrasound images were manually divided into inner medulla, outer medulla, and cortex, and each area was subdivided into arteries and veins. We quantified vascular density and tortuosity, number of detected microbubbles, and generated tracks. For comparison, we assessed glomerular filtration rate, albumin/creatinine ratio, and renal histology to evaluate CKD. The number of detected microbubbles and generated tracks varied between animals and significantly affected quantification of vessel density. In areas with a comparable number of tracks, density increased in the obese animals, concomitant with a decrease in glomerular filtration rate and an increase in albumin/creatinine ratio, but without any pathology in the histological staining. The results indicate that super-resolution ultrasound imaging can be used to quantify structural alterations in the renal vasculature. Techniques to generate more comparable number of microbubble tracks and confirmation of the findings in larger-scale studies are needed. MDPI 2022-07-04 /pmc/articles/PMC9318608/ /pubmed/35885531 http://dx.doi.org/10.3390/diagnostics12071626 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Søgaard, Stinne Byrholdt Andersen, Sofie Bech Taghavi, Iman Hoyos, Carlos Armando Villagómez Christoffersen, Christina Hansen, Kristoffer Lindskov Jensen, Jørgen Arendt Nielsen, Michael Bachmann Sørensen, Charlotte Mehlin Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study |
title | Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study |
title_full | Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study |
title_fullStr | Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study |
title_full_unstemmed | Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study |
title_short | Super-Resolution Ultrasound Imaging Provides Quantification of the Renal Cortical and Medullary Vasculature in Obese Zucker Rats: A Pilot Study |
title_sort | super-resolution ultrasound imaging provides quantification of the renal cortical and medullary vasculature in obese zucker rats: a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318608/ https://www.ncbi.nlm.nih.gov/pubmed/35885531 http://dx.doi.org/10.3390/diagnostics12071626 |
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