Cargando…

Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment

Understanding dynamic and complex interaction of biological membranes with extracellular matrices plays a crucial role in controlling a variety of cell behavior and functions, from cell adhesion and growth to signaling and differentiation. Tremendous interest in tissue engineering has made it possib...

Descripción completa

Detalles Bibliográficos
Autores principales: Karaz, Selcan, Han, Mertcan, Akay, Gizem, Onal, Asim, Nizamoglu, Sedat, Kizilel, Seda, Senses, Erkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318666/
https://www.ncbi.nlm.nih.gov/pubmed/35877843
http://dx.doi.org/10.3390/membranes12070640
_version_ 1784755349372272640
author Karaz, Selcan
Han, Mertcan
Akay, Gizem
Onal, Asim
Nizamoglu, Sedat
Kizilel, Seda
Senses, Erkan
author_facet Karaz, Selcan
Han, Mertcan
Akay, Gizem
Onal, Asim
Nizamoglu, Sedat
Kizilel, Seda
Senses, Erkan
author_sort Karaz, Selcan
collection PubMed
description Understanding dynamic and complex interaction of biological membranes with extracellular matrices plays a crucial role in controlling a variety of cell behavior and functions, from cell adhesion and growth to signaling and differentiation. Tremendous interest in tissue engineering has made it possible to design polymeric scaffolds mimicking the topology and mechanical properties of the native extracellular microenvironment; however, a fundamental question remains unanswered: that is, how the viscoelastic extracellular environment modifies the hierarchical dynamics of lipid membranes. In this work, we used aqueous solutions of poly(ethylene glycol) (PEG) with different molecular weights to mimic the viscous medium of cells and nearly monodisperse unilamellar DMPC/DMPG liposomes as a membrane model. Using small-angle X-ray scattering (SAXS), dynamic light scattering, temperature-modulated differential scanning calorimetry, bulk rheology, and fluorescence lifetime spectroscopy, we investigated the structural phase map and multiscale dynamics of the liposome–polymer mixtures. The results suggest an unprecedented dynamic coupling between polymer chains and phospholipid bilayers at different length/time scales. The microviscosity of the lipid bilayers is directly influenced by the relaxation of the whole chain, resulting in accelerated dynamics of lipids within the bilayers in the case of short chains compared to the polymer-free liposome case. At the macroscopic level, the gel-to-fluid transition of the bilayers results in a remarkable thermal-stiffening behavior of polymer–liposome solutions that can be modified by the concentration of the liposomes and the polymer chain length.
format Online
Article
Text
id pubmed-9318666
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93186662022-07-27 Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment Karaz, Selcan Han, Mertcan Akay, Gizem Onal, Asim Nizamoglu, Sedat Kizilel, Seda Senses, Erkan Membranes (Basel) Article Understanding dynamic and complex interaction of biological membranes with extracellular matrices plays a crucial role in controlling a variety of cell behavior and functions, from cell adhesion and growth to signaling and differentiation. Tremendous interest in tissue engineering has made it possible to design polymeric scaffolds mimicking the topology and mechanical properties of the native extracellular microenvironment; however, a fundamental question remains unanswered: that is, how the viscoelastic extracellular environment modifies the hierarchical dynamics of lipid membranes. In this work, we used aqueous solutions of poly(ethylene glycol) (PEG) with different molecular weights to mimic the viscous medium of cells and nearly monodisperse unilamellar DMPC/DMPG liposomes as a membrane model. Using small-angle X-ray scattering (SAXS), dynamic light scattering, temperature-modulated differential scanning calorimetry, bulk rheology, and fluorescence lifetime spectroscopy, we investigated the structural phase map and multiscale dynamics of the liposome–polymer mixtures. The results suggest an unprecedented dynamic coupling between polymer chains and phospholipid bilayers at different length/time scales. The microviscosity of the lipid bilayers is directly influenced by the relaxation of the whole chain, resulting in accelerated dynamics of lipids within the bilayers in the case of short chains compared to the polymer-free liposome case. At the macroscopic level, the gel-to-fluid transition of the bilayers results in a remarkable thermal-stiffening behavior of polymer–liposome solutions that can be modified by the concentration of the liposomes and the polymer chain length. MDPI 2022-06-21 /pmc/articles/PMC9318666/ /pubmed/35877843 http://dx.doi.org/10.3390/membranes12070640 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Karaz, Selcan
Han, Mertcan
Akay, Gizem
Onal, Asim
Nizamoglu, Sedat
Kizilel, Seda
Senses, Erkan
Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
title Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
title_full Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
title_fullStr Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
title_full_unstemmed Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
title_short Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
title_sort multiscale dynamics of lipid vesicles in polymeric microenvironment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318666/
https://www.ncbi.nlm.nih.gov/pubmed/35877843
http://dx.doi.org/10.3390/membranes12070640
work_keys_str_mv AT karazselcan multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment
AT hanmertcan multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment
AT akaygizem multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment
AT onalasim multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment
AT nizamoglusedat multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment
AT kizilelseda multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment
AT senseserkan multiscaledynamicsoflipidvesiclesinpolymericmicroenvironment