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Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde

This study aimed to investigate, for the first time, the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales in Cape Verde. A total of 98 inpatients hospitalized at Hospital Universitário Agostinho Neto were screened for rectal coloniz...

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Autores principales: Freire, Samanta, Grilo, Teresa, Teixeira, Maria Luísa, Fernandes, Euclides, Poirel, Laurent, Aires-de-Sousa, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318797/
https://www.ncbi.nlm.nih.gov/pubmed/35889144
http://dx.doi.org/10.3390/microorganisms10071426
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author Freire, Samanta
Grilo, Teresa
Teixeira, Maria Luísa
Fernandes, Euclides
Poirel, Laurent
Aires-de-Sousa, Marta
author_facet Freire, Samanta
Grilo, Teresa
Teixeira, Maria Luísa
Fernandes, Euclides
Poirel, Laurent
Aires-de-Sousa, Marta
author_sort Freire, Samanta
collection PubMed
description This study aimed to investigate, for the first time, the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales in Cape Verde. A total of 98 inpatients hospitalized at Hospital Universitário Agostinho Neto were screened for rectal colonization. All ESBL- and carbapenemase-producing isolates were tested for antimicrobial susceptibility and characterized by multilocus sequence typing. Mating-out assay followed by PCR-based replicon typing were performed to characterize the plasmids harboring carbapenemase encoding genes. A large proportion of patients carried ESBL- or carbapenemase-producing Enterobacterales (56% and 6%, respectively). Among 93 ESBL-producing isolates, there were mainly Klebsiella pneumoniae (58%) and Escherichia coli (37%). Five different ESBLs were detected, with CTX-M-15 being highly predominant (92%). Six carbapenemase-producing isolates (five E. coli and one K. pneumoniae) were recovered, and all of the OXA-48-like type (four OXA-181, one OXA-48, and one OXA-244). The bla(OXA-48) gene was located on an IncFI-type plasmid, the bla(OXA-181) gene on IncFI or IncX3 plasmids, and the bla(OXA-244) gene was found to be chromosomally located. The five carbapenemase-producing E. coli isolates belonged to five distinct sequence types. This study overall showed a very high prevalence of ESBL-producing Enterobacterales, as well as the emergence of carbapenemase producers in this hospital.
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spelling pubmed-93187972022-07-27 Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde Freire, Samanta Grilo, Teresa Teixeira, Maria Luísa Fernandes, Euclides Poirel, Laurent Aires-de-Sousa, Marta Microorganisms Article This study aimed to investigate, for the first time, the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales in Cape Verde. A total of 98 inpatients hospitalized at Hospital Universitário Agostinho Neto were screened for rectal colonization. All ESBL- and carbapenemase-producing isolates were tested for antimicrobial susceptibility and characterized by multilocus sequence typing. Mating-out assay followed by PCR-based replicon typing were performed to characterize the plasmids harboring carbapenemase encoding genes. A large proportion of patients carried ESBL- or carbapenemase-producing Enterobacterales (56% and 6%, respectively). Among 93 ESBL-producing isolates, there were mainly Klebsiella pneumoniae (58%) and Escherichia coli (37%). Five different ESBLs were detected, with CTX-M-15 being highly predominant (92%). Six carbapenemase-producing isolates (five E. coli and one K. pneumoniae) were recovered, and all of the OXA-48-like type (four OXA-181, one OXA-48, and one OXA-244). The bla(OXA-48) gene was located on an IncFI-type plasmid, the bla(OXA-181) gene on IncFI or IncX3 plasmids, and the bla(OXA-244) gene was found to be chromosomally located. The five carbapenemase-producing E. coli isolates belonged to five distinct sequence types. This study overall showed a very high prevalence of ESBL-producing Enterobacterales, as well as the emergence of carbapenemase producers in this hospital. MDPI 2022-07-14 /pmc/articles/PMC9318797/ /pubmed/35889144 http://dx.doi.org/10.3390/microorganisms10071426 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Freire, Samanta
Grilo, Teresa
Teixeira, Maria Luísa
Fernandes, Euclides
Poirel, Laurent
Aires-de-Sousa, Marta
Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde
title Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde
title_full Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde
title_fullStr Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde
title_full_unstemmed Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde
title_short Screening and Characterization of Multidrug-Resistant Enterobacterales among Hospitalized Patients in the African Archipelago of Cape Verde
title_sort screening and characterization of multidrug-resistant enterobacterales among hospitalized patients in the african archipelago of cape verde
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318797/
https://www.ncbi.nlm.nih.gov/pubmed/35889144
http://dx.doi.org/10.3390/microorganisms10071426
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