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Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness...

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Autores principales: Trovão, Mafalda, Schüler, Lisa M., Machado, Adriana, Bombo, Gabriel, Navalho, Sofia, Barros, Ana, Pereira, Hugo, Silva, Joana, Freitas, Filomena, Varela, João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318807/
https://www.ncbi.nlm.nih.gov/pubmed/35877733
http://dx.doi.org/10.3390/md20070440
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author Trovão, Mafalda
Schüler, Lisa M.
Machado, Adriana
Bombo, Gabriel
Navalho, Sofia
Barros, Ana
Pereira, Hugo
Silva, Joana
Freitas, Filomena
Varela, João
author_facet Trovão, Mafalda
Schüler, Lisa M.
Machado, Adriana
Bombo, Gabriel
Navalho, Sofia
Barros, Ana
Pereira, Hugo
Silva, Joana
Freitas, Filomena
Varela, João
author_sort Trovão, Mafalda
collection PubMed
description Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed.
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spelling pubmed-93188072022-07-27 Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production Trovão, Mafalda Schüler, Lisa M. Machado, Adriana Bombo, Gabriel Navalho, Sofia Barros, Ana Pereira, Hugo Silva, Joana Freitas, Filomena Varela, João Mar Drugs Review Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed. MDPI 2022-06-30 /pmc/articles/PMC9318807/ /pubmed/35877733 http://dx.doi.org/10.3390/md20070440 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Trovão, Mafalda
Schüler, Lisa M.
Machado, Adriana
Bombo, Gabriel
Navalho, Sofia
Barros, Ana
Pereira, Hugo
Silva, Joana
Freitas, Filomena
Varela, João
Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production
title Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production
title_full Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production
title_fullStr Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production
title_full_unstemmed Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production
title_short Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production
title_sort random mutagenesis as a promising tool for microalgal strain improvement towards industrial production
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318807/
https://www.ncbi.nlm.nih.gov/pubmed/35877733
http://dx.doi.org/10.3390/md20070440
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