Cargando…
Improvement of Gene Delivery and Mutation Efficiency in the CRISPR-Cas9 Wheat (Triticum aestivum L.) Genomics System via Biolistics
Discovery of the CRISPR-Cas9 gene editing system revolutionized the field of plant genomics. Despite advantages in the ease of designing gRNA and the low cost of the CRISPR-Cas9 system, there are still hurdles to overcome in low mutation efficiencies, specifically in hexaploid wheat. In conjunction...
Autores principales: | Tanaka, Jaclyn, Minkenberg, Bastian, Poddar, Snigdha, Staskawicz, Brian, Cho, Myeong-Je |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318839/ https://www.ncbi.nlm.nih.gov/pubmed/35885963 http://dx.doi.org/10.3390/genes13071180 |
Ejemplares similares
-
Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.)
por: Poddar, Snigdha, et al.
Publicado: (2023) -
Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat
por: Hamada, Haruyasu, et al.
Publicado: (2018) -
Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube
por: Nagahara, Shiori, et al.
Publicado: (2021) -
A Streamlined Protocol for Wheat (Triticum aestivum) Protoplast Isolation and Transformation With CRISPR-Cas Ribonucleoprotein Complexes
por: Brandt, Kali M., et al.
Publicado: (2020) -
An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue
por: Miller, Kyle, et al.
Publicado: (2021)