Triatoma guazu Lent and Wygodzinsky Is a Junior Synonym of Triatoma williami Galvão, Souza and Lima

SIMPLE SUMMARY: Triatomines are blood-sucking insects, potential vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma guazu and Triatoma williami are phylogenetically very close and occur in sympatry. Morphologic, morphometric, and genetic analyses were performed to discuss the taxonomic status of these species. Morphometric and molecular data do not show diagnostic characteristics between species, whereas their different patterns of connexival spots were considered a phenotypic polymorphism, common in triatomines. These results suggest T. guazu as a junior synonym of T. williami. Therefore, the synonym between these species is formally proposed here. ABSTRACT: Triatoma guazu Lent and Wygodzinsky and Triatoma williami Galvão, Souza, and Lima (Hemiptera: Triatominae) are found in human dwellings and are potential vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma guazu was described based solely on a single female specimen, from the municipality of Villarica, Guairá Department, Paraguay, and posteriorly, a male from Barra do Garças, Mato Grosso, Brazil was described and designated as the allotype of this species. Triatoma williami is found in the central-west of Brazil between Goiás, Mato Grosso, and Mato Grosso do Sul. However, the taxonomic “status” of these species is questioned. Previous studies indicate the lack of isoenzymatic diagnostic loci, morphometric similarity, low genetic divergence, and close evolutionary relationship of these species. In this study, we compared the morphology, morphometry, and mitochondrial DNA fragments of the populations of the two species. The morphological diagnostic characteristic among these species is the difference in the connexivum spots pattern, which has been recognized as a phenotypic variation that exists among populations resulting from ecological diversity. Furthermore, our analysis also revealed the morphometric similarity and low genetic divergence between these species. Therefore, in the present paper, we formally propose T. guazu as a junior synonym of T. williami.