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Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity

Fibrous membranes based on natural polymers obtained by the electrospinning technique are a great choice for wound dressings. In order to promote an efficient wound repair, and to avoid antibiotics, antibacterial plant extracts can be incorporated. In the present work, the new electrospun nanofibre...

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Autores principales: Suflet, Dana M., Popescu, Irina, Pelin, Irina M., David, Geta, Serbezeanu, Diana, Rîmbu, Cristina M., Daraba, Oana M., Enache, Alin A., Bercea, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319135/
https://www.ncbi.nlm.nih.gov/pubmed/35877524
http://dx.doi.org/10.3390/gels8070439
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author Suflet, Dana M.
Popescu, Irina
Pelin, Irina M.
David, Geta
Serbezeanu, Diana
Rîmbu, Cristina M.
Daraba, Oana M.
Enache, Alin A.
Bercea, Maria
author_facet Suflet, Dana M.
Popescu, Irina
Pelin, Irina M.
David, Geta
Serbezeanu, Diana
Rîmbu, Cristina M.
Daraba, Oana M.
Enache, Alin A.
Bercea, Maria
author_sort Suflet, Dana M.
collection PubMed
description Fibrous membranes based on natural polymers obtained by the electrospinning technique are a great choice for wound dressings. In order to promote an efficient wound repair, and to avoid antibiotics, antibacterial plant extracts can be incorporated. In the present work, the new electrospun nanofibre membranes based on monobasic phosphate curdlan (PCurd) and polyvinyl alcohol (PVA) were obtained for the first time. To establish the adequate mixing ratio for electrospinning, the behaviour of the PCurd and PVA mixture was studied by viscometry and rheology. In order to confer antimicrobial activity with the nanofibre membrane, clove essential oil (CEO) was incorporated into the electrospun solution. Well-defined and drop-free nanofibres with a diameter between 157 nm and 110 nm were obtained. The presence of CEO in the obtained nanofibres was confirmed by ATR–FTIR spectroscopy, by the phenolic and flavonoid contents, and by the antioxidant activity of the membranes. In physiological conditions, CEO was released from the membrane after 24 h. The in vivo antimicrobial tests showed a good inhibitory activity against E. coli and higher activity against S. aureus. Furthermore, the viability cell test showed the lack of cytotoxicity of the nanofibre membrane with and without CEO, confirming its potential use in wound treatment.
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spelling pubmed-93191352022-07-27 Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity Suflet, Dana M. Popescu, Irina Pelin, Irina M. David, Geta Serbezeanu, Diana Rîmbu, Cristina M. Daraba, Oana M. Enache, Alin A. Bercea, Maria Gels Article Fibrous membranes based on natural polymers obtained by the electrospinning technique are a great choice for wound dressings. In order to promote an efficient wound repair, and to avoid antibiotics, antibacterial plant extracts can be incorporated. In the present work, the new electrospun nanofibre membranes based on monobasic phosphate curdlan (PCurd) and polyvinyl alcohol (PVA) were obtained for the first time. To establish the adequate mixing ratio for electrospinning, the behaviour of the PCurd and PVA mixture was studied by viscometry and rheology. In order to confer antimicrobial activity with the nanofibre membrane, clove essential oil (CEO) was incorporated into the electrospun solution. Well-defined and drop-free nanofibres with a diameter between 157 nm and 110 nm were obtained. The presence of CEO in the obtained nanofibres was confirmed by ATR–FTIR spectroscopy, by the phenolic and flavonoid contents, and by the antioxidant activity of the membranes. In physiological conditions, CEO was released from the membrane after 24 h. The in vivo antimicrobial tests showed a good inhibitory activity against E. coli and higher activity against S. aureus. Furthermore, the viability cell test showed the lack of cytotoxicity of the nanofibre membrane with and without CEO, confirming its potential use in wound treatment. MDPI 2022-07-13 /pmc/articles/PMC9319135/ /pubmed/35877524 http://dx.doi.org/10.3390/gels8070439 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suflet, Dana M.
Popescu, Irina
Pelin, Irina M.
David, Geta
Serbezeanu, Diana
Rîmbu, Cristina M.
Daraba, Oana M.
Enache, Alin A.
Bercea, Maria
Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity
title Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity
title_full Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity
title_fullStr Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity
title_full_unstemmed Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity
title_short Phosphorylated Curdlan Gel/Polyvinyl Alcohol Electrospun Nanofibres Loaded with Clove Oil with Antibacterial Activity
title_sort phosphorylated curdlan gel/polyvinyl alcohol electrospun nanofibres loaded with clove oil with antibacterial activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319135/
https://www.ncbi.nlm.nih.gov/pubmed/35877524
http://dx.doi.org/10.3390/gels8070439
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