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Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model

Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as well as to agglomerate, which reduces the effectiveness of their use for the antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake structure, can be a carrier of AgNP that stabilizes their movem...

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Autores principales: Zielińska-Górska, Marlena, Sawosz, Ewa, Sosnowska, Malwina, Hotowy, Anna, Grodzik, Marta, Górski, Konrad, Strojny-Cieślak, Barbara, Wierzbicki, Mateusz, Chwalibog, André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319156/
https://www.ncbi.nlm.nih.gov/pubmed/35890292
http://dx.doi.org/10.3390/pharmaceutics14071398
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author Zielińska-Górska, Marlena
Sawosz, Ewa
Sosnowska, Malwina
Hotowy, Anna
Grodzik, Marta
Górski, Konrad
Strojny-Cieślak, Barbara
Wierzbicki, Mateusz
Chwalibog, André
author_facet Zielińska-Górska, Marlena
Sawosz, Ewa
Sosnowska, Malwina
Hotowy, Anna
Grodzik, Marta
Górski, Konrad
Strojny-Cieślak, Barbara
Wierzbicki, Mateusz
Chwalibog, André
author_sort Zielińska-Górska, Marlena
collection PubMed
description Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as well as to agglomerate, which reduces the effectiveness of their use for the antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake structure, can be a carrier of AgNP that stabilizes their movement without inhibiting their antibacterial properties. Considering that the human skin is often the first contact with antimicrobial agent, the aim of the study was to assess whether the application of the complex of AgNP and GO is biocompatible with the skin model in in vitro studies. The conducted tests were performed in accordance with the criteria set in OECD TG439. AgNP-GO complex did not influence the genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well as MIP1β (macrophage inflammatory protein 1β) expressed in the GO group. Moreover, it showed a positive effect on the micro- and ultra-structure of the skin model. In conclusion, the synergistic effect of AgNP and GO as a complex can activate the process of epidermis renewal, which makes it suitable for use as a material for skin contact.
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spelling pubmed-93191562022-07-27 Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model Zielińska-Górska, Marlena Sawosz, Ewa Sosnowska, Malwina Hotowy, Anna Grodzik, Marta Górski, Konrad Strojny-Cieślak, Barbara Wierzbicki, Mateusz Chwalibog, André Pharmaceutics Article Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as well as to agglomerate, which reduces the effectiveness of their use for the antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake structure, can be a carrier of AgNP that stabilizes their movement without inhibiting their antibacterial properties. Considering that the human skin is often the first contact with antimicrobial agent, the aim of the study was to assess whether the application of the complex of AgNP and GO is biocompatible with the skin model in in vitro studies. The conducted tests were performed in accordance with the criteria set in OECD TG439. AgNP-GO complex did not influence the genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well as MIP1β (macrophage inflammatory protein 1β) expressed in the GO group. Moreover, it showed a positive effect on the micro- and ultra-structure of the skin model. In conclusion, the synergistic effect of AgNP and GO as a complex can activate the process of epidermis renewal, which makes it suitable for use as a material for skin contact. MDPI 2022-07-01 /pmc/articles/PMC9319156/ /pubmed/35890292 http://dx.doi.org/10.3390/pharmaceutics14071398 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zielińska-Górska, Marlena
Sawosz, Ewa
Sosnowska, Malwina
Hotowy, Anna
Grodzik, Marta
Górski, Konrad
Strojny-Cieślak, Barbara
Wierzbicki, Mateusz
Chwalibog, André
Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
title Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
title_full Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
title_fullStr Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
title_full_unstemmed Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
title_short Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
title_sort molecular biocompatibility of a silver nanoparticle complex with graphene oxide to human skin in a 3d epidermis in vitro model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319156/
https://www.ncbi.nlm.nih.gov/pubmed/35890292
http://dx.doi.org/10.3390/pharmaceutics14071398
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