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Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus

Porcine astrovirus (PAstV) has been identified as an important diarrheic pathogen with a broad global distribution. The PAstV is a potential pathogen to human beings and plays a role in public health. Until now, the divergence characteristics and pathogenesis of the PAstV are still not well known. I...

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Autores principales: Tao, Jie, Li, Benqiang, Cheng, Jinghua, Shi, Ying, Qiao, Changtao, Lin, Zhi, Liu, Huili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319226/
https://www.ncbi.nlm.nih.gov/pubmed/35891364
http://dx.doi.org/10.3390/v14071383
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author Tao, Jie
Li, Benqiang
Cheng, Jinghua
Shi, Ying
Qiao, Changtao
Lin, Zhi
Liu, Huili
author_facet Tao, Jie
Li, Benqiang
Cheng, Jinghua
Shi, Ying
Qiao, Changtao
Lin, Zhi
Liu, Huili
author_sort Tao, Jie
collection PubMed
description Porcine astrovirus (PAstV) has been identified as an important diarrheic pathogen with a broad global distribution. The PAstV is a potential pathogen to human beings and plays a role in public health. Until now, the divergence characteristics and pathogenesis of the PAstV are still not well known. In this study, the PAstV-4 strain PAstV/CH/2022/CM1 was isolated from the diarrheal feces of a piglet in Shanghai, which was identified to be a recombination of PAstV4/JPN (LC201612) and PAstV4/CHN (JX060808). A time tree based on the ORF2 protein of the astrovirus demonstrated that type 2–5 PAstV (PAstV-2 to 5) diverged from type 1 PAstV (PAstV-1) at a point from 1992 to 2000. To better understand the molecular basis of the virus, we sought to explore the host cell response to the PAstV/CH/2022/CM1 infection using proteomics. The results demonstrate that viral infection elicits global protein changes, and that the mitochondria seems to be a primary and an important target in viral infection. Importantly, there was crosstalk between autophagy and apoptosis, in which ATG7 might be the key mediator. In addition, the NOD-like receptor X1 (NLRX1) in the mitochondria was activated and participated in several important antiviral signaling pathways after the PAstV/CH/2022/CM1 infection, which was closely related to mitophagy. The NLRX1 may be a crucial protein for antagonizing a viral infection through autophagy, but this has yet to be validated. In conclusion, the data in this study provides more information for understanding the virus genomic characterization and the potential antiviral targets in a PAstV infection.
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spelling pubmed-93192262022-07-27 Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus Tao, Jie Li, Benqiang Cheng, Jinghua Shi, Ying Qiao, Changtao Lin, Zhi Liu, Huili Viruses Article Porcine astrovirus (PAstV) has been identified as an important diarrheic pathogen with a broad global distribution. The PAstV is a potential pathogen to human beings and plays a role in public health. Until now, the divergence characteristics and pathogenesis of the PAstV are still not well known. In this study, the PAstV-4 strain PAstV/CH/2022/CM1 was isolated from the diarrheal feces of a piglet in Shanghai, which was identified to be a recombination of PAstV4/JPN (LC201612) and PAstV4/CHN (JX060808). A time tree based on the ORF2 protein of the astrovirus demonstrated that type 2–5 PAstV (PAstV-2 to 5) diverged from type 1 PAstV (PAstV-1) at a point from 1992 to 2000. To better understand the molecular basis of the virus, we sought to explore the host cell response to the PAstV/CH/2022/CM1 infection using proteomics. The results demonstrate that viral infection elicits global protein changes, and that the mitochondria seems to be a primary and an important target in viral infection. Importantly, there was crosstalk between autophagy and apoptosis, in which ATG7 might be the key mediator. In addition, the NOD-like receptor X1 (NLRX1) in the mitochondria was activated and participated in several important antiviral signaling pathways after the PAstV/CH/2022/CM1 infection, which was closely related to mitophagy. The NLRX1 may be a crucial protein for antagonizing a viral infection through autophagy, but this has yet to be validated. In conclusion, the data in this study provides more information for understanding the virus genomic characterization and the potential antiviral targets in a PAstV infection. MDPI 2022-06-24 /pmc/articles/PMC9319226/ /pubmed/35891364 http://dx.doi.org/10.3390/v14071383 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tao, Jie
Li, Benqiang
Cheng, Jinghua
Shi, Ying
Qiao, Changtao
Lin, Zhi
Liu, Huili
Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus
title Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus
title_full Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus
title_fullStr Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus
title_full_unstemmed Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus
title_short Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus
title_sort genomic divergence characterization and quantitative proteomics exploration of type 4 porcine astrovirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319226/
https://www.ncbi.nlm.nih.gov/pubmed/35891364
http://dx.doi.org/10.3390/v14071383
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