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Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts

Damage to mesenchymal cells occurs by dental implant drills as a consequence of shear forces and heat generation. However, how the damaged mesenchymal cells can affect the polarization of macrophages and their differentiation into osteoclastogenesis is not fully understood. To simulate cell damage,...

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Autores principales: Panahipour, Layla, Abbasabadi, Azarakhsh Oladzad, Kaiser, Viktoria, Sordi, Mariane Beatriz, Kargarpour, Zahra, Gruber, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319356/
https://www.ncbi.nlm.nih.gov/pubmed/35887825
http://dx.doi.org/10.3390/jcm11144061
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author Panahipour, Layla
Abbasabadi, Azarakhsh Oladzad
Kaiser, Viktoria
Sordi, Mariane Beatriz
Kargarpour, Zahra
Gruber, Reinhard
author_facet Panahipour, Layla
Abbasabadi, Azarakhsh Oladzad
Kaiser, Viktoria
Sordi, Mariane Beatriz
Kargarpour, Zahra
Gruber, Reinhard
author_sort Panahipour, Layla
collection PubMed
description Damage to mesenchymal cells occurs by dental implant drills as a consequence of shear forces and heat generation. However, how the damaged mesenchymal cells can affect the polarization of macrophages and their differentiation into osteoclastogenesis is not fully understood. To simulate cell damage, we exposed suspended ST2 murine bone marrow stromal cells to freeze/thawing or sonication cycles, followed by centrifugation. We then evaluated the lysates for their capacity to modulate lipopolysaccharide-induced macrophage polarization and RANKL-MCSF-TGF-β-induced osteoclastogenesis. We report that lysates of ST2, particularly when sonicated, greatly diminished the expression of inflammatory IL6 and COX2 as well as moderately increased arginase 1 in primary macrophages. That was confirmed by lysates obtained from the osteocytic cell line IDG-SW3. Moreover, the ST2 lysate lowered the phosphorylation of p65 and p38 as well as the nuclear translocation of p65. We further show herein that lysates of damaged ST2 reduced the formation of osteoclast-like cells characterized by their multinuclearity and the expression of tartrate-resistant phosphatase and cathepsin K. Taken together, our data suggest that thermal and mechanical damage of mesenchymal cells causes the release of as-yet-to-be-defined molecules that dampen an inflammatory response and the formation of osteoclasts in vitro.
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spelling pubmed-93193562022-07-27 Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts Panahipour, Layla Abbasabadi, Azarakhsh Oladzad Kaiser, Viktoria Sordi, Mariane Beatriz Kargarpour, Zahra Gruber, Reinhard J Clin Med Article Damage to mesenchymal cells occurs by dental implant drills as a consequence of shear forces and heat generation. However, how the damaged mesenchymal cells can affect the polarization of macrophages and their differentiation into osteoclastogenesis is not fully understood. To simulate cell damage, we exposed suspended ST2 murine bone marrow stromal cells to freeze/thawing or sonication cycles, followed by centrifugation. We then evaluated the lysates for their capacity to modulate lipopolysaccharide-induced macrophage polarization and RANKL-MCSF-TGF-β-induced osteoclastogenesis. We report that lysates of ST2, particularly when sonicated, greatly diminished the expression of inflammatory IL6 and COX2 as well as moderately increased arginase 1 in primary macrophages. That was confirmed by lysates obtained from the osteocytic cell line IDG-SW3. Moreover, the ST2 lysate lowered the phosphorylation of p65 and p38 as well as the nuclear translocation of p65. We further show herein that lysates of damaged ST2 reduced the formation of osteoclast-like cells characterized by their multinuclearity and the expression of tartrate-resistant phosphatase and cathepsin K. Taken together, our data suggest that thermal and mechanical damage of mesenchymal cells causes the release of as-yet-to-be-defined molecules that dampen an inflammatory response and the formation of osteoclasts in vitro. MDPI 2022-07-14 /pmc/articles/PMC9319356/ /pubmed/35887825 http://dx.doi.org/10.3390/jcm11144061 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panahipour, Layla
Abbasabadi, Azarakhsh Oladzad
Kaiser, Viktoria
Sordi, Mariane Beatriz
Kargarpour, Zahra
Gruber, Reinhard
Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts
title Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts
title_full Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts
title_fullStr Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts
title_full_unstemmed Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts
title_short Damaged Mesenchymal Cells Dampen the Inflammatory Response of Macrophages and the Formation of Osteoclasts
title_sort damaged mesenchymal cells dampen the inflammatory response of macrophages and the formation of osteoclasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319356/
https://www.ncbi.nlm.nih.gov/pubmed/35887825
http://dx.doi.org/10.3390/jcm11144061
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