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Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity

Diabetes mellitus (DM) and its complications are a severe public health concern due to the high incidence, morbidity, and mortality rates. The present study aims to synthesize and characterize silver nanoparticles (AgNPs) using the aqueous leaf extract of Psidium guajava (PGE) for investigating its...

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Autores principales: Nagaraja, Sreeharsha, Ahmed, Syed Sagheer, D. R., Bharathi, Goudanavar, Prakash, M., Rupesh Kumar, Fattepur, Santosh, Meravanige, Girish, Shariff, Arshia, Shiroorkar, Predeepkumar Narayanappa, Habeebuddin, Mohammed, Telsang, Mallikarjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319605/
https://www.ncbi.nlm.nih.gov/pubmed/35889209
http://dx.doi.org/10.3390/molecules27144336
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author Nagaraja, Sreeharsha
Ahmed, Syed Sagheer
D. R., Bharathi
Goudanavar, Prakash
M., Rupesh Kumar
Fattepur, Santosh
Meravanige, Girish
Shariff, Arshia
Shiroorkar, Predeepkumar Narayanappa
Habeebuddin, Mohammed
Telsang, Mallikarjun
author_facet Nagaraja, Sreeharsha
Ahmed, Syed Sagheer
D. R., Bharathi
Goudanavar, Prakash
M., Rupesh Kumar
Fattepur, Santosh
Meravanige, Girish
Shariff, Arshia
Shiroorkar, Predeepkumar Narayanappa
Habeebuddin, Mohammed
Telsang, Mallikarjun
author_sort Nagaraja, Sreeharsha
collection PubMed
description Diabetes mellitus (DM) and its complications are a severe public health concern due to the high incidence, morbidity, and mortality rates. The present study aims to synthesize and characterize silver nanoparticles (AgNPs) using the aqueous leaf extract of Psidium guajava (PGE) for investigating its antidiabetic activity. Psidium guajava silver nanoparticles (PGAg NPs) were prepared and characterized by various parameters. The in vivo study was conducted using PGE and PGAg NPs in Streptozotocin (STZ)-induced diabetic rats to assess their antidiabetic properties. STZ of 55 mg/kg was injected to induce diabetes. The PGE, PGAg NPs at a dose of 200 and 400 mg/kg and standard drug Metformin (100 mg/kg) were administered daily to diabetic rats for 21 days through the oral route. Blood glucose level, body weight changes, lipid profiles, and histopathology of the rats’ liver and pancreas were examined. In the diabetic rats, PGE and PGAg NPs produced a drastic decrease in the blood glucose level, preventing subsequent weight loss and ameliorating lipid profile parameters. The histopathological findings revealed the improvements in pancreas and liver cells due to the repercussion of PGE and PGAg NPs. A compelling effect was observed in all doses of PGE and PGAg NPs; however, PGAg NPs exhibited a more promising result. Thus, from the results, it is concluded that the synthesized PGAg NPs has potent antidiabetic activity due to its enhanced surface area and smaller particle size of nanoparticles.
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spelling pubmed-93196052022-07-27 Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity Nagaraja, Sreeharsha Ahmed, Syed Sagheer D. R., Bharathi Goudanavar, Prakash M., Rupesh Kumar Fattepur, Santosh Meravanige, Girish Shariff, Arshia Shiroorkar, Predeepkumar Narayanappa Habeebuddin, Mohammed Telsang, Mallikarjun Molecules Article Diabetes mellitus (DM) and its complications are a severe public health concern due to the high incidence, morbidity, and mortality rates. The present study aims to synthesize and characterize silver nanoparticles (AgNPs) using the aqueous leaf extract of Psidium guajava (PGE) for investigating its antidiabetic activity. Psidium guajava silver nanoparticles (PGAg NPs) were prepared and characterized by various parameters. The in vivo study was conducted using PGE and PGAg NPs in Streptozotocin (STZ)-induced diabetic rats to assess their antidiabetic properties. STZ of 55 mg/kg was injected to induce diabetes. The PGE, PGAg NPs at a dose of 200 and 400 mg/kg and standard drug Metformin (100 mg/kg) were administered daily to diabetic rats for 21 days through the oral route. Blood glucose level, body weight changes, lipid profiles, and histopathology of the rats’ liver and pancreas were examined. In the diabetic rats, PGE and PGAg NPs produced a drastic decrease in the blood glucose level, preventing subsequent weight loss and ameliorating lipid profile parameters. The histopathological findings revealed the improvements in pancreas and liver cells due to the repercussion of PGE and PGAg NPs. A compelling effect was observed in all doses of PGE and PGAg NPs; however, PGAg NPs exhibited a more promising result. Thus, from the results, it is concluded that the synthesized PGAg NPs has potent antidiabetic activity due to its enhanced surface area and smaller particle size of nanoparticles. MDPI 2022-07-06 /pmc/articles/PMC9319605/ /pubmed/35889209 http://dx.doi.org/10.3390/molecules27144336 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagaraja, Sreeharsha
Ahmed, Syed Sagheer
D. R., Bharathi
Goudanavar, Prakash
M., Rupesh Kumar
Fattepur, Santosh
Meravanige, Girish
Shariff, Arshia
Shiroorkar, Predeepkumar Narayanappa
Habeebuddin, Mohammed
Telsang, Mallikarjun
Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity
title Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity
title_full Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity
title_fullStr Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity
title_full_unstemmed Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity
title_short Green Synthesis and Characterization of Silver Nanoparticles of Psidium guajava Leaf Extract and Evaluation for Its Antidiabetic Activity
title_sort green synthesis and characterization of silver nanoparticles of psidium guajava leaf extract and evaluation for its antidiabetic activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319605/
https://www.ncbi.nlm.nih.gov/pubmed/35889209
http://dx.doi.org/10.3390/molecules27144336
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