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Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis

This study aimed to evaluate the relationship of esophageal epithelial permeability with mast cell infiltration and IgG4 deposits as well as chemokine levels in eosinophilic esophagitis (EoE) patients before and after treatment. Biopsies from controls and EoE patients before and after treatment were...

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Autores principales: Chen, Junji, Oshima, Tadayuki, Huang, Xinyi, Tomita, Toshihiko, Fukui, Hirokazu, Miwa, Hiroto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319696/
https://www.ncbi.nlm.nih.gov/pubmed/35888006
http://dx.doi.org/10.3390/jcm11144246
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author Chen, Junji
Oshima, Tadayuki
Huang, Xinyi
Tomita, Toshihiko
Fukui, Hirokazu
Miwa, Hiroto
author_facet Chen, Junji
Oshima, Tadayuki
Huang, Xinyi
Tomita, Toshihiko
Fukui, Hirokazu
Miwa, Hiroto
author_sort Chen, Junji
collection PubMed
description This study aimed to evaluate the relationship of esophageal epithelial permeability with mast cell infiltration and IgG4 deposits as well as chemokine levels in eosinophilic esophagitis (EoE) patients before and after treatment. Biopsies from controls and EoE patients before and after treatment were analyzed. Hematoxylin and eosin staining was used to show eosinophil infiltration. Paracellular permeability of the esophageal epithelium was assessed using surface biotinylation. Immunohistochemical staining was performed to examine mast cell infiltration and IgG4 deposits. Gene expression of chemokines was evaluated by qRT-PCR. Esophageal epithelial infiltration of mast cells, IgG4 deposits, and permeability were significantly increased in EoE patients. Levels of interleukin-13, calpain-14, and eotaxin-3 mRNAs were significantly upregulated, while filaggrin, serine peptidase inhibitor Kazal type 7 (SPINK7), and involucrin mRNAs were significantly downregulated in EoE patients. In patients achieving histologic remission diagnosed by eosinophil counts, a subset of EoE patients with unchanged permeability after treatment showed increases in mast cell infiltration, IgG4 deposits, and interleukin-13, calpain-14, filaggrin, and SPINK7 expression, with decreased eotaxin-3 and involucrin. Other EoE patients with decreased permeability displayed decreased eotaxin-3, involucrin, and mast cell infiltration, no IgG4 deposits, and increased IL-13, calpain-14, filaggrin, and SPINK7. Increased permeability of the esophagus in EoE patients without eosinophil infiltration after treatment was associated with mast cell infiltration and IgG4 deposits.
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spelling pubmed-93196962022-07-27 Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis Chen, Junji Oshima, Tadayuki Huang, Xinyi Tomita, Toshihiko Fukui, Hirokazu Miwa, Hiroto J Clin Med Article This study aimed to evaluate the relationship of esophageal epithelial permeability with mast cell infiltration and IgG4 deposits as well as chemokine levels in eosinophilic esophagitis (EoE) patients before and after treatment. Biopsies from controls and EoE patients before and after treatment were analyzed. Hematoxylin and eosin staining was used to show eosinophil infiltration. Paracellular permeability of the esophageal epithelium was assessed using surface biotinylation. Immunohistochemical staining was performed to examine mast cell infiltration and IgG4 deposits. Gene expression of chemokines was evaluated by qRT-PCR. Esophageal epithelial infiltration of mast cells, IgG4 deposits, and permeability were significantly increased in EoE patients. Levels of interleukin-13, calpain-14, and eotaxin-3 mRNAs were significantly upregulated, while filaggrin, serine peptidase inhibitor Kazal type 7 (SPINK7), and involucrin mRNAs were significantly downregulated in EoE patients. In patients achieving histologic remission diagnosed by eosinophil counts, a subset of EoE patients with unchanged permeability after treatment showed increases in mast cell infiltration, IgG4 deposits, and interleukin-13, calpain-14, filaggrin, and SPINK7 expression, with decreased eotaxin-3 and involucrin. Other EoE patients with decreased permeability displayed decreased eotaxin-3, involucrin, and mast cell infiltration, no IgG4 deposits, and increased IL-13, calpain-14, filaggrin, and SPINK7. Increased permeability of the esophagus in EoE patients without eosinophil infiltration after treatment was associated with mast cell infiltration and IgG4 deposits. MDPI 2022-07-21 /pmc/articles/PMC9319696/ /pubmed/35888006 http://dx.doi.org/10.3390/jcm11144246 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Junji
Oshima, Tadayuki
Huang, Xinyi
Tomita, Toshihiko
Fukui, Hirokazu
Miwa, Hiroto
Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis
title Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis
title_full Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis
title_fullStr Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis
title_full_unstemmed Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis
title_short Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis
title_sort esophageal mucosal permeability as a surrogate measure of cure in eosinophilic esophagitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319696/
https://www.ncbi.nlm.nih.gov/pubmed/35888006
http://dx.doi.org/10.3390/jcm11144246
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