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Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers
Urinary catheter infections remain an issue for many patients and can complicate their health status, especially for individuals who require long-term catheterization. Catheters can be colonized by biofilm-forming bacteria resistant to the administered antibiotics. Therefore, this study aimed to inv...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319761/ https://www.ncbi.nlm.nih.gov/pubmed/35889016 http://dx.doi.org/10.3390/microorganisms10071297 |
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author | Rugaie, Osamah Al Abdellatif, Ahmed A. H. El-Mokhtar, Mohamed A. Sabet, Marwa A. Abdelfattah, Ahmed Alsharidah, Mansour Aldubaib, Musaed Barakat, Hassan Abudoleh, Suha Mujahed Al-Regaiey, Khalid A. Tawfeek, Hesham M. |
author_facet | Rugaie, Osamah Al Abdellatif, Ahmed A. H. El-Mokhtar, Mohamed A. Sabet, Marwa A. Abdelfattah, Ahmed Alsharidah, Mansour Aldubaib, Musaed Barakat, Hassan Abudoleh, Suha Mujahed Al-Regaiey, Khalid A. Tawfeek, Hesham M. |
author_sort | Rugaie, Osamah Al |
collection | PubMed |
description | Urinary catheter infections remain an issue for many patients and can complicate their health status, especially for individuals who require long-term catheterization. Catheters can be colonized by biofilm-forming bacteria resistant to the administered antibiotics. Therefore, this study aimed to investigate the efficacy of silver nanoparticles (AgNPs) stabilized with different polymeric materials generated via a one-step simple coating technique for their ability to inhibit biofilm formation on urinary catheters. AgNPs were prepared and characterized to confirm their formation and determine their size, charge, morphology, and physical stability. Screening of the antimicrobial activity of nanoparticle formulations and determining minimal inhibitory concentration (MIC) and their cytotoxicity against PC3 cells were performed. Moreover, the antibiofilm activity and efficacy of the AgNPs coated on the urinary catheters under static and flowing conditions were examined against a clinical isolate of Escherichia coli. The results showed that the investigated polymers could form physically stable AgNPs, especially those prepared using polyvinyl pyrrolidone (PVP) and ethyl cellulose (EC). Preliminary screening and MIC determinations suggested that the AgNPs-EC and AgNPs-PVP had superior antibacterial effects against E. coli. AgNPs-EC and AgNPs-PVP inhibited biofilm formation to 58.2% and 50.8% compared with AgNPs-PEG, silver nitrate solution and control samples. In addition, coating urinary catheters with AgNPs-EC and AgNPs-PVP at concentrations lower than the determined IC50 values significantly (p < 0.05; t-test) inhibited bacterial biofilm formation compared with noncoated catheters under both static and static and flowing conditions using two different types of commercial Foley urinary catheters. The data obtained in this study provide evidence that AgNP-coated EC and PVP could be useful as potential antibacterial and antibiofilm catheter coating agents to prevent the development of urinary tract infections caused by E. coli. |
format | Online Article Text |
id | pubmed-9319761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93197612022-07-27 Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers Rugaie, Osamah Al Abdellatif, Ahmed A. H. El-Mokhtar, Mohamed A. Sabet, Marwa A. Abdelfattah, Ahmed Alsharidah, Mansour Aldubaib, Musaed Barakat, Hassan Abudoleh, Suha Mujahed Al-Regaiey, Khalid A. Tawfeek, Hesham M. Microorganisms Article Urinary catheter infections remain an issue for many patients and can complicate their health status, especially for individuals who require long-term catheterization. Catheters can be colonized by biofilm-forming bacteria resistant to the administered antibiotics. Therefore, this study aimed to investigate the efficacy of silver nanoparticles (AgNPs) stabilized with different polymeric materials generated via a one-step simple coating technique for their ability to inhibit biofilm formation on urinary catheters. AgNPs were prepared and characterized to confirm their formation and determine their size, charge, morphology, and physical stability. Screening of the antimicrobial activity of nanoparticle formulations and determining minimal inhibitory concentration (MIC) and their cytotoxicity against PC3 cells were performed. Moreover, the antibiofilm activity and efficacy of the AgNPs coated on the urinary catheters under static and flowing conditions were examined against a clinical isolate of Escherichia coli. The results showed that the investigated polymers could form physically stable AgNPs, especially those prepared using polyvinyl pyrrolidone (PVP) and ethyl cellulose (EC). Preliminary screening and MIC determinations suggested that the AgNPs-EC and AgNPs-PVP had superior antibacterial effects against E. coli. AgNPs-EC and AgNPs-PVP inhibited biofilm formation to 58.2% and 50.8% compared with AgNPs-PEG, silver nitrate solution and control samples. In addition, coating urinary catheters with AgNPs-EC and AgNPs-PVP at concentrations lower than the determined IC50 values significantly (p < 0.05; t-test) inhibited bacterial biofilm formation compared with noncoated catheters under both static and static and flowing conditions using two different types of commercial Foley urinary catheters. The data obtained in this study provide evidence that AgNP-coated EC and PVP could be useful as potential antibacterial and antibiofilm catheter coating agents to prevent the development of urinary tract infections caused by E. coli. MDPI 2022-06-27 /pmc/articles/PMC9319761/ /pubmed/35889016 http://dx.doi.org/10.3390/microorganisms10071297 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rugaie, Osamah Al Abdellatif, Ahmed A. H. El-Mokhtar, Mohamed A. Sabet, Marwa A. Abdelfattah, Ahmed Alsharidah, Mansour Aldubaib, Musaed Barakat, Hassan Abudoleh, Suha Mujahed Al-Regaiey, Khalid A. Tawfeek, Hesham M. Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers |
title | Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers |
title_full | Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers |
title_fullStr | Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers |
title_full_unstemmed | Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers |
title_short | Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers |
title_sort | retardation of bacterial biofilm formation by coating urinary catheters with metal nanoparticle-stabilized polymers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319761/ https://www.ncbi.nlm.nih.gov/pubmed/35889016 http://dx.doi.org/10.3390/microorganisms10071297 |
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