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Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan

The World Health Organization initiated a global surveillance system for respiratory syncytial virus (RSV) in 2015, and the pilot surveillance is ongoing. The real-time RT-PCR RSV assays (Pan-RSV and duplex assays) developed by the United States Centers for Disease Control and Prevention are applied...

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Autores principales: Suwa, Reiko, Kume, Yohei, Kawase, Miyuki, Chishiki, Mina, Ono, Takashi, Norito, Sakurako, Sato, Ko, Okamoto, Michiko, Kumaki, Satoru, Nagai, Yukio, Hosoya, Mitsuaki, Takeda, Makoto, Nishimura, Hidekazu, Hashimoto, Koichi, Shirato, Kazuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319774/
https://www.ncbi.nlm.nih.gov/pubmed/35889999
http://dx.doi.org/10.3390/pathogens11070754
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author Suwa, Reiko
Kume, Yohei
Kawase, Miyuki
Chishiki, Mina
Ono, Takashi
Norito, Sakurako
Sato, Ko
Okamoto, Michiko
Kumaki, Satoru
Nagai, Yukio
Hosoya, Mitsuaki
Takeda, Makoto
Nishimura, Hidekazu
Hashimoto, Koichi
Shirato, Kazuya
author_facet Suwa, Reiko
Kume, Yohei
Kawase, Miyuki
Chishiki, Mina
Ono, Takashi
Norito, Sakurako
Sato, Ko
Okamoto, Michiko
Kumaki, Satoru
Nagai, Yukio
Hosoya, Mitsuaki
Takeda, Makoto
Nishimura, Hidekazu
Hashimoto, Koichi
Shirato, Kazuya
author_sort Suwa, Reiko
collection PubMed
description The World Health Organization initiated a global surveillance system for respiratory syncytial virus (RSV) in 2015, and the pilot surveillance is ongoing. The real-time RT-PCR RSV assays (Pan-RSV and duplex assays) developed by the United States Centers for Disease Control and Prevention are applied as the standard assays. To introduce these as standard assays in Japan, their practicality was evaluated using 2261 specimens obtained from pediatric inpatients in Japan, which were collected from 2018 to 2021. Although the Pan-RSV and duplex assays had similar analytical sensitivities, they yielded 630 (27.9%) and 786 (34.8%) RSV-positive specimens, respectively (p < 0.001). Although sequencing analysis showed mismatches in the reverse primer used in the Pan-RSV assay, these mismatches did not affect its analytical sensitivity. The analysis of read numbers of RSV isolates from air–liquid interface culture of human bronchial/tracheal epithelial cells showed that the duplex assay had a greater number of reads than did the Pan-RSV assay. Therefore, the duplex assay has superior detection performance compared with the Pan-RSV assay, but the two assays have similar analytical sensitivities.
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spelling pubmed-93197742022-07-27 Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan Suwa, Reiko Kume, Yohei Kawase, Miyuki Chishiki, Mina Ono, Takashi Norito, Sakurako Sato, Ko Okamoto, Michiko Kumaki, Satoru Nagai, Yukio Hosoya, Mitsuaki Takeda, Makoto Nishimura, Hidekazu Hashimoto, Koichi Shirato, Kazuya Pathogens Article The World Health Organization initiated a global surveillance system for respiratory syncytial virus (RSV) in 2015, and the pilot surveillance is ongoing. The real-time RT-PCR RSV assays (Pan-RSV and duplex assays) developed by the United States Centers for Disease Control and Prevention are applied as the standard assays. To introduce these as standard assays in Japan, their practicality was evaluated using 2261 specimens obtained from pediatric inpatients in Japan, which were collected from 2018 to 2021. Although the Pan-RSV and duplex assays had similar analytical sensitivities, they yielded 630 (27.9%) and 786 (34.8%) RSV-positive specimens, respectively (p < 0.001). Although sequencing analysis showed mismatches in the reverse primer used in the Pan-RSV assay, these mismatches did not affect its analytical sensitivity. The analysis of read numbers of RSV isolates from air–liquid interface culture of human bronchial/tracheal epithelial cells showed that the duplex assay had a greater number of reads than did the Pan-RSV assay. Therefore, the duplex assay has superior detection performance compared with the Pan-RSV assay, but the two assays have similar analytical sensitivities. MDPI 2022-07-01 /pmc/articles/PMC9319774/ /pubmed/35889999 http://dx.doi.org/10.3390/pathogens11070754 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suwa, Reiko
Kume, Yohei
Kawase, Miyuki
Chishiki, Mina
Ono, Takashi
Norito, Sakurako
Sato, Ko
Okamoto, Michiko
Kumaki, Satoru
Nagai, Yukio
Hosoya, Mitsuaki
Takeda, Makoto
Nishimura, Hidekazu
Hashimoto, Koichi
Shirato, Kazuya
Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan
title Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan
title_full Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan
title_fullStr Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan
title_full_unstemmed Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan
title_short Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan
title_sort practical validation of united states centers for disease control and prevention assays for the detection of human respiratory syncytial virus in pediatric inpatients in japan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319774/
https://www.ncbi.nlm.nih.gov/pubmed/35889999
http://dx.doi.org/10.3390/pathogens11070754
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