Cargando…
Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov.
The metagenome of foulings from sulfidic spring “Serovodorodny” (Tatarstan, Russia), where members of the genus Thiothrix was observed, was sequenced. Representatives of the phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in the microbial community. The complete genome of Th...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319827/ https://www.ncbi.nlm.nih.gov/pubmed/35889019 http://dx.doi.org/10.3390/microorganisms10071300 |
_version_ | 1784755644938584064 |
---|---|
author | Ravin, Nikolai V. Rossetti, Simona Beletsky, Alexey V. Kadnikov, Vitaly V. Rudenko, Tatyana S. Smolyakov, Dmitry D. Moskvitina, Marina I. Gureeva, Maria V. Mardanov, Andrey V. Grabovich, Margarita Yu. |
author_facet | Ravin, Nikolai V. Rossetti, Simona Beletsky, Alexey V. Kadnikov, Vitaly V. Rudenko, Tatyana S. Smolyakov, Dmitry D. Moskvitina, Marina I. Gureeva, Maria V. Mardanov, Andrey V. Grabovich, Margarita Yu. |
author_sort | Ravin, Nikolai V. |
collection | PubMed |
description | The metagenome of foulings from sulfidic spring “Serovodorodny” (Tatarstan, Russia), where members of the genus Thiothrix was observed, was sequenced. Representatives of the phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in the microbial community. The complete genome of Thiothrix sp. KT was assembled from the metagenome. It displayed 93.93–99.72% 16S rRNA gene sequence identity to other Thiothrix species. The average nucleotide identity (ANI) и digital DNA-DNA hybridization (dDDH) showed that the genome designated KT represents a new species within the genus Thiothrix, ‘Candidatus Thiothrix sulfatifontis’ sp. nov. KT. The taxonomic status has been determined of the strain Thiothrix sp. CT3, isolated about 30 years ago and not assigned to any of Thiothrix species due to high 16S rRNA gene sequence identity with related species (i.e., 98.8–99.4%). The complete genome sequence of strain CT3 was determined. The ANI between CT3 and other Thiothrix species was below 82%, and the dDDH values were less than 40%, indicating that strain CT3 belongs to a novel species, Thiothrix winogradskyi sp. nov. A genome analysis showed that both strains are chemo-organoheterotrophs, chemolithotrophs (in the presence of hydrogen sulfide and thiosulfate) and chemoautotrophs. For the first time, representatives of Thiothrix showed anaerobic growth in the presence of thiosulfate. |
format | Online Article Text |
id | pubmed-9319827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93198272022-07-27 Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. Ravin, Nikolai V. Rossetti, Simona Beletsky, Alexey V. Kadnikov, Vitaly V. Rudenko, Tatyana S. Smolyakov, Dmitry D. Moskvitina, Marina I. Gureeva, Maria V. Mardanov, Andrey V. Grabovich, Margarita Yu. Microorganisms Article The metagenome of foulings from sulfidic spring “Serovodorodny” (Tatarstan, Russia), where members of the genus Thiothrix was observed, was sequenced. Representatives of the phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in the microbial community. The complete genome of Thiothrix sp. KT was assembled from the metagenome. It displayed 93.93–99.72% 16S rRNA gene sequence identity to other Thiothrix species. The average nucleotide identity (ANI) и digital DNA-DNA hybridization (dDDH) showed that the genome designated KT represents a new species within the genus Thiothrix, ‘Candidatus Thiothrix sulfatifontis’ sp. nov. KT. The taxonomic status has been determined of the strain Thiothrix sp. CT3, isolated about 30 years ago and not assigned to any of Thiothrix species due to high 16S rRNA gene sequence identity with related species (i.e., 98.8–99.4%). The complete genome sequence of strain CT3 was determined. The ANI between CT3 and other Thiothrix species was below 82%, and the dDDH values were less than 40%, indicating that strain CT3 belongs to a novel species, Thiothrix winogradskyi sp. nov. A genome analysis showed that both strains are chemo-organoheterotrophs, chemolithotrophs (in the presence of hydrogen sulfide and thiosulfate) and chemoautotrophs. For the first time, representatives of Thiothrix showed anaerobic growth in the presence of thiosulfate. MDPI 2022-06-27 /pmc/articles/PMC9319827/ /pubmed/35889019 http://dx.doi.org/10.3390/microorganisms10071300 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ravin, Nikolai V. Rossetti, Simona Beletsky, Alexey V. Kadnikov, Vitaly V. Rudenko, Tatyana S. Smolyakov, Dmitry D. Moskvitina, Marina I. Gureeva, Maria V. Mardanov, Andrey V. Grabovich, Margarita Yu. Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. |
title | Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. |
title_full | Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. |
title_fullStr | Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. |
title_full_unstemmed | Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. |
title_short | Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov. |
title_sort | two new species of filamentous sulfur bacteria of the genus thiothrix, thiothrix winogradskyi sp. nov. and ‘candidatus thiothrix sulfatifontis’ sp. nov. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319827/ https://www.ncbi.nlm.nih.gov/pubmed/35889019 http://dx.doi.org/10.3390/microorganisms10071300 |
work_keys_str_mv | AT ravinnikolaiv twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT rossettisimona twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT beletskyalexeyv twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT kadnikovvitalyv twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT rudenkotatyanas twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT smolyakovdmitryd twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT moskvitinamarinai twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT gureevamariav twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT mardanovandreyv twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov AT grabovichmargaritayu twonewspeciesoffilamentoussulfurbacteriaofthegenusthiothrixthiothrixwinogradskyispnovandcandidatusthiothrixsulfatifontisspnov |