Cargando…

Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture

Numerous cardiovascular diseases (CVD) eventually lead to severe myocardial dysfunction, which is the most common cause of death worldwide. A better understanding of underlying molecular mechanisms of cardiovascular pathologies seems to be crucial to develop effective therapeutic options. Therefore,...

Descripción completa

Detalles Bibliográficos
Autores principales: Nawrocki, Mariusz J., Jopek, Karol, Kaczmarek, Mariusz, Zdun, Maciej, Mozdziak, Paul, Jemielity, Marek, Perek, Bartłomiej, Bukowska, Dorota, Kempisty, Bartosz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319992/
https://www.ncbi.nlm.nih.gov/pubmed/35885988
http://dx.doi.org/10.3390/genes13071205
_version_ 1784755684886183936
author Nawrocki, Mariusz J.
Jopek, Karol
Kaczmarek, Mariusz
Zdun, Maciej
Mozdziak, Paul
Jemielity, Marek
Perek, Bartłomiej
Bukowska, Dorota
Kempisty, Bartosz
author_facet Nawrocki, Mariusz J.
Jopek, Karol
Kaczmarek, Mariusz
Zdun, Maciej
Mozdziak, Paul
Jemielity, Marek
Perek, Bartłomiej
Bukowska, Dorota
Kempisty, Bartosz
author_sort Nawrocki, Mariusz J.
collection PubMed
description Numerous cardiovascular diseases (CVD) eventually lead to severe myocardial dysfunction, which is the most common cause of death worldwide. A better understanding of underlying molecular mechanisms of cardiovascular pathologies seems to be crucial to develop effective therapeutic options. Therefore, a worthwhile endeavor is a detailed molecular characterization of cells extracted from the myocardium. A transcriptomic profile of atrial cardiomyocytes during long-term primary cell culture revealed the expression patterns depending on the duration of the culture and the heart segment of origin (right atrial appendage and right atrium). Differentially expressed genes (DEGs) were classified as involved in ontological groups such as: “cellular component assembly”, “cellular component organization”, “cellular component biogenesis”, and “cytoskeleton organization”. Transcriptomic profiling allowed us to indicate the increased expression of COL5A2, COL8A1, and COL12A1, encoding different collagen subunits, pivotal in cardiac extracellular matrix (ECM) structure. Conversely, genes important for cellular architecture, such as ABLIM1, TMOD1, XIRP1, and PHACTR1, were downregulated during in vitro culture. The culture conditions may create a favorable environment for reconstruction of the ECM structures, whereas they may be suboptimal for expression of some pivotal transcripts responsible for the formation of intracellular structures.
format Online
Article
Text
id pubmed-9319992
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93199922022-07-27 Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture Nawrocki, Mariusz J. Jopek, Karol Kaczmarek, Mariusz Zdun, Maciej Mozdziak, Paul Jemielity, Marek Perek, Bartłomiej Bukowska, Dorota Kempisty, Bartosz Genes (Basel) Article Numerous cardiovascular diseases (CVD) eventually lead to severe myocardial dysfunction, which is the most common cause of death worldwide. A better understanding of underlying molecular mechanisms of cardiovascular pathologies seems to be crucial to develop effective therapeutic options. Therefore, a worthwhile endeavor is a detailed molecular characterization of cells extracted from the myocardium. A transcriptomic profile of atrial cardiomyocytes during long-term primary cell culture revealed the expression patterns depending on the duration of the culture and the heart segment of origin (right atrial appendage and right atrium). Differentially expressed genes (DEGs) were classified as involved in ontological groups such as: “cellular component assembly”, “cellular component organization”, “cellular component biogenesis”, and “cytoskeleton organization”. Transcriptomic profiling allowed us to indicate the increased expression of COL5A2, COL8A1, and COL12A1, encoding different collagen subunits, pivotal in cardiac extracellular matrix (ECM) structure. Conversely, genes important for cellular architecture, such as ABLIM1, TMOD1, XIRP1, and PHACTR1, were downregulated during in vitro culture. The culture conditions may create a favorable environment for reconstruction of the ECM structures, whereas they may be suboptimal for expression of some pivotal transcripts responsible for the formation of intracellular structures. MDPI 2022-07-05 /pmc/articles/PMC9319992/ /pubmed/35885988 http://dx.doi.org/10.3390/genes13071205 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nawrocki, Mariusz J.
Jopek, Karol
Kaczmarek, Mariusz
Zdun, Maciej
Mozdziak, Paul
Jemielity, Marek
Perek, Bartłomiej
Bukowska, Dorota
Kempisty, Bartosz
Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
title Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
title_full Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
title_fullStr Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
title_full_unstemmed Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
title_short Transcriptomic Profile of Genes Regulating the Structural Organization of Porcine Atrial Cardiomyocytes during Primary In Vitro Culture
title_sort transcriptomic profile of genes regulating the structural organization of porcine atrial cardiomyocytes during primary in vitro culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319992/
https://www.ncbi.nlm.nih.gov/pubmed/35885988
http://dx.doi.org/10.3390/genes13071205
work_keys_str_mv AT nawrockimariuszj transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT jopekkarol transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT kaczmarekmariusz transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT zdunmaciej transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT mozdziakpaul transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT jemielitymarek transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT perekbartłomiej transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT bukowskadorota transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture
AT kempistybartosz transcriptomicprofileofgenesregulatingthestructuralorganizationofporcineatrialcardiomyocytesduringprimaryinvitroculture