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Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis

Polyunsaturated fatty acids (PUFA) hypersensitize yeast to oxidative stress. Ethanol accumulation during fermentation is another factor that induces oxidative stress via mitochondrial dysfunction and ROS overproduction. Since this microorganism has raised growing interest as a PUFA factory, we have...

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Autores principales: Olmos-Orizaba, Berenice Eridani, Arroyo-Peñaloza, José Santos, Martínez-Alcántar, Lorena, Montoya-Pérez, Rocío, Flores-García, Alberto, Rodríguez-Orozco, Alain Raimundo, Calderón-Cortés, Elizabeth, Saavedra-Molina, Alfredo, Campos-García, Jesús, Cortés-Rojo, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320082/
https://www.ncbi.nlm.nih.gov/pubmed/35888140
http://dx.doi.org/10.3390/life12071052
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author Olmos-Orizaba, Berenice Eridani
Arroyo-Peñaloza, José Santos
Martínez-Alcántar, Lorena
Montoya-Pérez, Rocío
Flores-García, Alberto
Rodríguez-Orozco, Alain Raimundo
Calderón-Cortés, Elizabeth
Saavedra-Molina, Alfredo
Campos-García, Jesús
Cortés-Rojo, Christian
author_facet Olmos-Orizaba, Berenice Eridani
Arroyo-Peñaloza, José Santos
Martínez-Alcántar, Lorena
Montoya-Pérez, Rocío
Flores-García, Alberto
Rodríguez-Orozco, Alain Raimundo
Calderón-Cortés, Elizabeth
Saavedra-Molina, Alfredo
Campos-García, Jesús
Cortés-Rojo, Christian
author_sort Olmos-Orizaba, Berenice Eridani
collection PubMed
description Polyunsaturated fatty acids (PUFA) hypersensitize yeast to oxidative stress. Ethanol accumulation during fermentation is another factor that induces oxidative stress via mitochondrial dysfunction and ROS overproduction. Since this microorganism has raised growing interest as a PUFA factory, we have studied if the combination of PUFA plus ethanol enhances yeast death. Respiration, ROS generation, lipid peroxidation, mitochondrial cardiolipin content, and cell death were assessed in yeast grown in the presence of 10% ethanol (ETOH) or linolenic acid (C18:3), or ethanol plus C18:3 (ETOH+C18:3). Lipid peroxidation and cardiolipin loss were several-fold higher in cells with ETOH+C18:3 than with C18:3. On the contrary, ETOH tended to increase cardiolipin content without inducing changes in lipid peroxidation. This was consistent with a remarkable diminution of cell growth and an exacerbated propidium iodide staining in cells with only ETOH+C18:3. The respiration rate decreased with all the treatments to a similar degree, and this was paralleled with similar increments in ROS between all the treatments. These results indicate that PUFA plus ethanol hypersensitize yeast to necrotic cell death by exacerbating membrane damage and mitochondrial cardiolipin loss, independent of mitochondrial dysfunction and ROS generation. The implications of these observations for some biotechnological applications in yeast and its physiology are discussed.
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spelling pubmed-93200822022-07-27 Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis Olmos-Orizaba, Berenice Eridani Arroyo-Peñaloza, José Santos Martínez-Alcántar, Lorena Montoya-Pérez, Rocío Flores-García, Alberto Rodríguez-Orozco, Alain Raimundo Calderón-Cortés, Elizabeth Saavedra-Molina, Alfredo Campos-García, Jesús Cortés-Rojo, Christian Life (Basel) Article Polyunsaturated fatty acids (PUFA) hypersensitize yeast to oxidative stress. Ethanol accumulation during fermentation is another factor that induces oxidative stress via mitochondrial dysfunction and ROS overproduction. Since this microorganism has raised growing interest as a PUFA factory, we have studied if the combination of PUFA plus ethanol enhances yeast death. Respiration, ROS generation, lipid peroxidation, mitochondrial cardiolipin content, and cell death were assessed in yeast grown in the presence of 10% ethanol (ETOH) or linolenic acid (C18:3), or ethanol plus C18:3 (ETOH+C18:3). Lipid peroxidation and cardiolipin loss were several-fold higher in cells with ETOH+C18:3 than with C18:3. On the contrary, ETOH tended to increase cardiolipin content without inducing changes in lipid peroxidation. This was consistent with a remarkable diminution of cell growth and an exacerbated propidium iodide staining in cells with only ETOH+C18:3. The respiration rate decreased with all the treatments to a similar degree, and this was paralleled with similar increments in ROS between all the treatments. These results indicate that PUFA plus ethanol hypersensitize yeast to necrotic cell death by exacerbating membrane damage and mitochondrial cardiolipin loss, independent of mitochondrial dysfunction and ROS generation. The implications of these observations for some biotechnological applications in yeast and its physiology are discussed. MDPI 2022-07-14 /pmc/articles/PMC9320082/ /pubmed/35888140 http://dx.doi.org/10.3390/life12071052 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olmos-Orizaba, Berenice Eridani
Arroyo-Peñaloza, José Santos
Martínez-Alcántar, Lorena
Montoya-Pérez, Rocío
Flores-García, Alberto
Rodríguez-Orozco, Alain Raimundo
Calderón-Cortés, Elizabeth
Saavedra-Molina, Alfredo
Campos-García, Jesús
Cortés-Rojo, Christian
Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis
title Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis
title_full Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis
title_fullStr Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis
title_full_unstemmed Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis
title_short Linolenic Acid Plus Ethanol Exacerbates Cell Death in Saccharomyces cerevisiae by Promoting Lipid Peroxidation, Cardiolipin Loss, and Necrosis
title_sort linolenic acid plus ethanol exacerbates cell death in saccharomyces cerevisiae by promoting lipid peroxidation, cardiolipin loss, and necrosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320082/
https://www.ncbi.nlm.nih.gov/pubmed/35888140
http://dx.doi.org/10.3390/life12071052
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