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Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
Bacillus Calmette–Guérin (BCG) vaccine is an attenuated strain of Mycobacterium bovis that provides weak protection against tuberculosis (TB). Mast cells (MCs) are tissue-resident immune cells strategically that serve as the first line of defence against pathogenic threats. In this study, we investi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320129/ https://www.ncbi.nlm.nih.gov/pubmed/35886897 http://dx.doi.org/10.3390/ijms23147549 |
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author | Garcia-Rodriguez, Karen M. Goenka, Anu Thomson, Darren D. Bahri, Rajia Tontini, Chiara Salcman, Barbora Hernandez-Pando, Rogelio Bulfone-Paus, Silvia |
author_facet | Garcia-Rodriguez, Karen M. Goenka, Anu Thomson, Darren D. Bahri, Rajia Tontini, Chiara Salcman, Barbora Hernandez-Pando, Rogelio Bulfone-Paus, Silvia |
author_sort | Garcia-Rodriguez, Karen M. |
collection | PubMed |
description | Bacillus Calmette–Guérin (BCG) vaccine is an attenuated strain of Mycobacterium bovis that provides weak protection against tuberculosis (TB). Mast cells (MCs) are tissue-resident immune cells strategically that serve as the first line of defence against pathogenic threats. In this study, we investigated the response of human MCs (hMCs) to BCG. We found that naïve hMCs exposed to BCG did not secrete cytokines, degranulate, or support the uptake and intracellular growth of bacteria. Since we could show that in hMCs IL-33 promotes the transcription of host-pathogen interaction, cell adhesion and activation genes, we used IL-33 for cell priming. The treatment of hMCs with IL-33, but not IFN-γ, before BCG stimulation increased IL-8, MCP-1 and IL-13 secretion, and induced an enhanced expression of the mycobacteria-binding receptor CD48. These effects were comparable to those caused by the recombinant Mycobacterium tuberculosis (Mtb) 19-KDa lipoprotein. Finally, stimulation of hMCs with IL-33 incremented MC-BCG interactions. Thus, we propose that IL-33 may improve the immunogenicity of BCG vaccine by sensitising hMCs. |
format | Online Article Text |
id | pubmed-9320129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93201292022-07-27 Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming Garcia-Rodriguez, Karen M. Goenka, Anu Thomson, Darren D. Bahri, Rajia Tontini, Chiara Salcman, Barbora Hernandez-Pando, Rogelio Bulfone-Paus, Silvia Int J Mol Sci Article Bacillus Calmette–Guérin (BCG) vaccine is an attenuated strain of Mycobacterium bovis that provides weak protection against tuberculosis (TB). Mast cells (MCs) are tissue-resident immune cells strategically that serve as the first line of defence against pathogenic threats. In this study, we investigated the response of human MCs (hMCs) to BCG. We found that naïve hMCs exposed to BCG did not secrete cytokines, degranulate, or support the uptake and intracellular growth of bacteria. Since we could show that in hMCs IL-33 promotes the transcription of host-pathogen interaction, cell adhesion and activation genes, we used IL-33 for cell priming. The treatment of hMCs with IL-33, but not IFN-γ, before BCG stimulation increased IL-8, MCP-1 and IL-13 secretion, and induced an enhanced expression of the mycobacteria-binding receptor CD48. These effects were comparable to those caused by the recombinant Mycobacterium tuberculosis (Mtb) 19-KDa lipoprotein. Finally, stimulation of hMCs with IL-33 incremented MC-BCG interactions. Thus, we propose that IL-33 may improve the immunogenicity of BCG vaccine by sensitising hMCs. MDPI 2022-07-07 /pmc/articles/PMC9320129/ /pubmed/35886897 http://dx.doi.org/10.3390/ijms23147549 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Garcia-Rodriguez, Karen M. Goenka, Anu Thomson, Darren D. Bahri, Rajia Tontini, Chiara Salcman, Barbora Hernandez-Pando, Rogelio Bulfone-Paus, Silvia Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming |
title | Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming |
title_full | Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming |
title_fullStr | Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming |
title_full_unstemmed | Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming |
title_short | Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming |
title_sort | bacillus calmette–guérin-induced human mast cell activation relies on il-33 priming |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320129/ https://www.ncbi.nlm.nih.gov/pubmed/35886897 http://dx.doi.org/10.3390/ijms23147549 |
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