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Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming

Bacillus Calmette–Guérin (BCG) vaccine is an attenuated strain of Mycobacterium bovis that provides weak protection against tuberculosis (TB). Mast cells (MCs) are tissue-resident immune cells strategically that serve as the first line of defence against pathogenic threats. In this study, we investi...

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Autores principales: Garcia-Rodriguez, Karen M., Goenka, Anu, Thomson, Darren D., Bahri, Rajia, Tontini, Chiara, Salcman, Barbora, Hernandez-Pando, Rogelio, Bulfone-Paus, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320129/
https://www.ncbi.nlm.nih.gov/pubmed/35886897
http://dx.doi.org/10.3390/ijms23147549
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author Garcia-Rodriguez, Karen M.
Goenka, Anu
Thomson, Darren D.
Bahri, Rajia
Tontini, Chiara
Salcman, Barbora
Hernandez-Pando, Rogelio
Bulfone-Paus, Silvia
author_facet Garcia-Rodriguez, Karen M.
Goenka, Anu
Thomson, Darren D.
Bahri, Rajia
Tontini, Chiara
Salcman, Barbora
Hernandez-Pando, Rogelio
Bulfone-Paus, Silvia
author_sort Garcia-Rodriguez, Karen M.
collection PubMed
description Bacillus Calmette–Guérin (BCG) vaccine is an attenuated strain of Mycobacterium bovis that provides weak protection against tuberculosis (TB). Mast cells (MCs) are tissue-resident immune cells strategically that serve as the first line of defence against pathogenic threats. In this study, we investigated the response of human MCs (hMCs) to BCG. We found that naïve hMCs exposed to BCG did not secrete cytokines, degranulate, or support the uptake and intracellular growth of bacteria. Since we could show that in hMCs IL-33 promotes the transcription of host-pathogen interaction, cell adhesion and activation genes, we used IL-33 for cell priming. The treatment of hMCs with IL-33, but not IFN-γ, before BCG stimulation increased IL-8, MCP-1 and IL-13 secretion, and induced an enhanced expression of the mycobacteria-binding receptor CD48. These effects were comparable to those caused by the recombinant Mycobacterium tuberculosis (Mtb) 19-KDa lipoprotein. Finally, stimulation of hMCs with IL-33 incremented MC-BCG interactions. Thus, we propose that IL-33 may improve the immunogenicity of BCG vaccine by sensitising hMCs.
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spelling pubmed-93201292022-07-27 Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming Garcia-Rodriguez, Karen M. Goenka, Anu Thomson, Darren D. Bahri, Rajia Tontini, Chiara Salcman, Barbora Hernandez-Pando, Rogelio Bulfone-Paus, Silvia Int J Mol Sci Article Bacillus Calmette–Guérin (BCG) vaccine is an attenuated strain of Mycobacterium bovis that provides weak protection against tuberculosis (TB). Mast cells (MCs) are tissue-resident immune cells strategically that serve as the first line of defence against pathogenic threats. In this study, we investigated the response of human MCs (hMCs) to BCG. We found that naïve hMCs exposed to BCG did not secrete cytokines, degranulate, or support the uptake and intracellular growth of bacteria. Since we could show that in hMCs IL-33 promotes the transcription of host-pathogen interaction, cell adhesion and activation genes, we used IL-33 for cell priming. The treatment of hMCs with IL-33, but not IFN-γ, before BCG stimulation increased IL-8, MCP-1 and IL-13 secretion, and induced an enhanced expression of the mycobacteria-binding receptor CD48. These effects were comparable to those caused by the recombinant Mycobacterium tuberculosis (Mtb) 19-KDa lipoprotein. Finally, stimulation of hMCs with IL-33 incremented MC-BCG interactions. Thus, we propose that IL-33 may improve the immunogenicity of BCG vaccine by sensitising hMCs. MDPI 2022-07-07 /pmc/articles/PMC9320129/ /pubmed/35886897 http://dx.doi.org/10.3390/ijms23147549 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia-Rodriguez, Karen M.
Goenka, Anu
Thomson, Darren D.
Bahri, Rajia
Tontini, Chiara
Salcman, Barbora
Hernandez-Pando, Rogelio
Bulfone-Paus, Silvia
Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
title Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
title_full Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
title_fullStr Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
title_full_unstemmed Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
title_short Bacillus Calmette–Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming
title_sort bacillus calmette–guérin-induced human mast cell activation relies on il-33 priming
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320129/
https://www.ncbi.nlm.nih.gov/pubmed/35886897
http://dx.doi.org/10.3390/ijms23147549
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