Cargando…

Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells

OBJECTIVE(S): Exosomes became the subject of extensive research in drug delivery approach due to their potential applicability as therapeutic tools for cancer therapy. Thymoquinone (Tq) is an anti-cancer agent due to its great anti-proliferative effect. However, poor solubility and weak bioavailabil...

Descripción completa

Detalles Bibliográficos
Autores principales: Ebrahimian, Mahboubeh, Hashemi, Maryam, Etemad, Leila, Salmasi, Zahra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320205/
https://www.ncbi.nlm.nih.gov/pubmed/35949303
http://dx.doi.org/10.22038/IJBMS.2022.64092.14116
_version_ 1784755736201396224
author Ebrahimian, Mahboubeh
Hashemi, Maryam
Etemad, Leila
Salmasi, Zahra
author_facet Ebrahimian, Mahboubeh
Hashemi, Maryam
Etemad, Leila
Salmasi, Zahra
author_sort Ebrahimian, Mahboubeh
collection PubMed
description OBJECTIVE(S): Exosomes became the subject of extensive research in drug delivery approach due to their potential applicability as therapeutic tools for cancer therapy. Thymoquinone (Tq) is an anti-cancer agent due to its great anti-proliferative effect. However, poor solubility and weak bioavailability restrict its therapeutic applications. In this study, exosomes secreted from human adipocyte-derived mesenchymal stem cells (AdMSCs) were isolated and the efficacy of a novel encapsulation method for loading of Tq was investigated. Finally, the cytotoxic effect of Tq incorporated exosomes against cancer cells was evaluated. MATERIALS AND METHODS: Exosomes secreted from AdMSCs were isolated via ultracentrifugation and characterized by electron microscopy and western blotting. Then, through a novel encapsulation approach, Tq was loaded into exosomes by the combination of three methods including incubation, freeze-thawing, and surfactant treatment. Then, the encapsulation efficiency, in vitro cellular uptake, and cytotoxicity of Tq incorporated exosomes (Tq@EXOs) in MCF7 and L929 cells were estimated. RESULTS: Tq loading into exosomes through our novel method caused a significant improvement in encapsulation efficiency of about 60%. The fluorescent microscopy and flow cytometry outcomes indicated the efficient uptake of Tq@EXOs-FITC by cells throughout 4 hr. Furthermore, MTT results displayed the ability of Tq@EXOs in effectively decreasing the cell viability of MCF7 without causing any obvious cytotoxicity on L929 as normal cells. CONCLUSION: The results suggest that our approach provides effective loading of Tq into exosomes which offer a valuable and safe platform for drug delivery to cancer cells thus having a great potential for clinical studies.
format Online
Article
Text
id pubmed-9320205
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-93202052022-08-09 Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells Ebrahimian, Mahboubeh Hashemi, Maryam Etemad, Leila Salmasi, Zahra Iran J Basic Med Sci Original Article OBJECTIVE(S): Exosomes became the subject of extensive research in drug delivery approach due to their potential applicability as therapeutic tools for cancer therapy. Thymoquinone (Tq) is an anti-cancer agent due to its great anti-proliferative effect. However, poor solubility and weak bioavailability restrict its therapeutic applications. In this study, exosomes secreted from human adipocyte-derived mesenchymal stem cells (AdMSCs) were isolated and the efficacy of a novel encapsulation method for loading of Tq was investigated. Finally, the cytotoxic effect of Tq incorporated exosomes against cancer cells was evaluated. MATERIALS AND METHODS: Exosomes secreted from AdMSCs were isolated via ultracentrifugation and characterized by electron microscopy and western blotting. Then, through a novel encapsulation approach, Tq was loaded into exosomes by the combination of three methods including incubation, freeze-thawing, and surfactant treatment. Then, the encapsulation efficiency, in vitro cellular uptake, and cytotoxicity of Tq incorporated exosomes (Tq@EXOs) in MCF7 and L929 cells were estimated. RESULTS: Tq loading into exosomes through our novel method caused a significant improvement in encapsulation efficiency of about 60%. The fluorescent microscopy and flow cytometry outcomes indicated the efficient uptake of Tq@EXOs-FITC by cells throughout 4 hr. Furthermore, MTT results displayed the ability of Tq@EXOs in effectively decreasing the cell viability of MCF7 without causing any obvious cytotoxicity on L929 as normal cells. CONCLUSION: The results suggest that our approach provides effective loading of Tq into exosomes which offer a valuable and safe platform for drug delivery to cancer cells thus having a great potential for clinical studies. Mashhad University of Medical Sciences 2022-06 /pmc/articles/PMC9320205/ /pubmed/35949303 http://dx.doi.org/10.22038/IJBMS.2022.64092.14116 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ebrahimian, Mahboubeh
Hashemi, Maryam
Etemad, Leila
Salmasi, Zahra
Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
title Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
title_full Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
title_fullStr Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
title_full_unstemmed Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
title_short Thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
title_sort thymoquinone-loaded mesenchymal stem cell-derived exosome as an efficient nano-system against breast cancer cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320205/
https://www.ncbi.nlm.nih.gov/pubmed/35949303
http://dx.doi.org/10.22038/IJBMS.2022.64092.14116
work_keys_str_mv AT ebrahimianmahboubeh thymoquinoneloadedmesenchymalstemcellderivedexosomeasanefficientnanosystemagainstbreastcancercells
AT hashemimaryam thymoquinoneloadedmesenchymalstemcellderivedexosomeasanefficientnanosystemagainstbreastcancercells
AT etemadleila thymoquinoneloadedmesenchymalstemcellderivedexosomeasanefficientnanosystemagainstbreastcancercells
AT salmasizahra thymoquinoneloadedmesenchymalstemcellderivedexosomeasanefficientnanosystemagainstbreastcancercells