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A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line

Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and s...

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Autores principales: Pilotti, Elisabetta, Cannata, Attilio, Magnani, Giacomo, Bignami, Fabio, Corsi, Andrea, Valenti, Maria Teresa, Shallak, Mariam, Forlani, Greta, Romanelli, Maria Grazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320395/
https://www.ncbi.nlm.nih.gov/pubmed/35886938
http://dx.doi.org/10.3390/ijms23147583
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author Pilotti, Elisabetta
Cannata, Attilio
Magnani, Giacomo
Bignami, Fabio
Corsi, Andrea
Valenti, Maria Teresa
Shallak, Mariam
Forlani, Greta
Romanelli, Maria Grazia
author_facet Pilotti, Elisabetta
Cannata, Attilio
Magnani, Giacomo
Bignami, Fabio
Corsi, Andrea
Valenti, Maria Teresa
Shallak, Mariam
Forlani, Greta
Romanelli, Maria Grazia
author_sort Pilotti, Elisabetta
collection PubMed
description Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and several mechanisms underlying HTLV-2 infection remain unexplored as well. Cellular miRNAs are key factors in the post-transcriptional regulation of gene expression and they are known to be potential targets for several pathogens to control the host microenvironment and, in particular, escape immune responses. Here, we identified a HTLV-2-related signature of eight miRNAs (miR-125a-3p, miR-381-3p, miR-502-5p, miR-708-5p, miR-548d-5p, miR-548c-5p, miR-1-3p, and miR-511-5p) in both HTLV-2 infected PBMC and BJABGu cell lines. Altered miRNA expression patterns were correlated with the impairment of Th cell differentiation and signaling pathways driven by cytokines and transcriptional factors such as the Runt-related transcription factor (RUNX) family members. Specifically, we demonstrated that the RUNX2 protein was significantly more expressed in the presence of Tax-2 compared with Tax-1 in an in vitro cell model. To the best of our knowledge, these data represent the first contribution to elucidating the HTLV-2 mediated alteration of host cell miRNA profiles that may impact on HTLV-2 replication and persistent infection.
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spelling pubmed-93203952022-07-27 A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line Pilotti, Elisabetta Cannata, Attilio Magnani, Giacomo Bignami, Fabio Corsi, Andrea Valenti, Maria Teresa Shallak, Mariam Forlani, Greta Romanelli, Maria Grazia Int J Mol Sci Article Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and several mechanisms underlying HTLV-2 infection remain unexplored as well. Cellular miRNAs are key factors in the post-transcriptional regulation of gene expression and they are known to be potential targets for several pathogens to control the host microenvironment and, in particular, escape immune responses. Here, we identified a HTLV-2-related signature of eight miRNAs (miR-125a-3p, miR-381-3p, miR-502-5p, miR-708-5p, miR-548d-5p, miR-548c-5p, miR-1-3p, and miR-511-5p) in both HTLV-2 infected PBMC and BJABGu cell lines. Altered miRNA expression patterns were correlated with the impairment of Th cell differentiation and signaling pathways driven by cytokines and transcriptional factors such as the Runt-related transcription factor (RUNX) family members. Specifically, we demonstrated that the RUNX2 protein was significantly more expressed in the presence of Tax-2 compared with Tax-1 in an in vitro cell model. To the best of our knowledge, these data represent the first contribution to elucidating the HTLV-2 mediated alteration of host cell miRNA profiles that may impact on HTLV-2 replication and persistent infection. MDPI 2022-07-08 /pmc/articles/PMC9320395/ /pubmed/35886938 http://dx.doi.org/10.3390/ijms23147583 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pilotti, Elisabetta
Cannata, Attilio
Magnani, Giacomo
Bignami, Fabio
Corsi, Andrea
Valenti, Maria Teresa
Shallak, Mariam
Forlani, Greta
Romanelli, Maria Grazia
A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line
title A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line
title_full A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line
title_fullStr A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line
title_full_unstemmed A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line
title_short A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line
title_sort panel of eight mirnas is deregulated in htlv-2 infected pbmcs and bjabgu cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320395/
https://www.ncbi.nlm.nih.gov/pubmed/35886938
http://dx.doi.org/10.3390/ijms23147583
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