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Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)

Diseases of the muscle tissue, particularly those disorders which result from the pathology of individual muscle cells, are often called myopathies. The diversity of the content of individual cells is of interest with regard to their role in both biochemical mechanisms and the structure of muscle ti...

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Autores principales: Kasprzyk, Paula, Wróbel, Paweł M., Dudała, Joanna, Geraki, Kalotina, Szczerbowska-Boruchowska, Magdalena, Radwańska, Edyta, Krzyżewski, Roger M., Adamek, Dariusz, Lankosz, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320641/
https://www.ncbi.nlm.nih.gov/pubmed/35887280
http://dx.doi.org/10.3390/ijms23147931
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author Kasprzyk, Paula
Wróbel, Paweł M.
Dudała, Joanna
Geraki, Kalotina
Szczerbowska-Boruchowska, Magdalena
Radwańska, Edyta
Krzyżewski, Roger M.
Adamek, Dariusz
Lankosz, Marek
author_facet Kasprzyk, Paula
Wróbel, Paweł M.
Dudała, Joanna
Geraki, Kalotina
Szczerbowska-Boruchowska, Magdalena
Radwańska, Edyta
Krzyżewski, Roger M.
Adamek, Dariusz
Lankosz, Marek
author_sort Kasprzyk, Paula
collection PubMed
description Diseases of the muscle tissue, particularly those disorders which result from the pathology of individual muscle cells, are often called myopathies. The diversity of the content of individual cells is of interest with regard to their role in both biochemical mechanisms and the structure of muscle tissue itself. These studies focus on the preliminary analysis of the differences that may occur between diseased tissues and tissues that have been recognised as a reference group. To do so, 13 samples of biopsied human muscle tissues were studied: 3 diagnosed as dystrophies, 6 as (non-dystrophic) myopathy and 4 regarded as references. From these sets of muscle biopsies, 135 completely measured muscle fibres were separated altogether, which were subjected to investigations using synchrotron radiation X-ray fluorescence (SR-XRF). Muscle fibres were analysed in terms of the composition of elements such as Br, Ca, Cl, Cr, Cu, Fe, K, Mn, P, S and Zn. The performed statistical tests indicate that all three groups (dystrophies—D; myopathies—M; references—R) show statistically significant differences in their elemental compositions, and the greatest impact, according to the multivariate discriminate analysis (MDA), comes from elements such as Ca, Cu, K, Cl and S.
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spelling pubmed-93206412022-07-27 Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF) Kasprzyk, Paula Wróbel, Paweł M. Dudała, Joanna Geraki, Kalotina Szczerbowska-Boruchowska, Magdalena Radwańska, Edyta Krzyżewski, Roger M. Adamek, Dariusz Lankosz, Marek Int J Mol Sci Article Diseases of the muscle tissue, particularly those disorders which result from the pathology of individual muscle cells, are often called myopathies. The diversity of the content of individual cells is of interest with regard to their role in both biochemical mechanisms and the structure of muscle tissue itself. These studies focus on the preliminary analysis of the differences that may occur between diseased tissues and tissues that have been recognised as a reference group. To do so, 13 samples of biopsied human muscle tissues were studied: 3 diagnosed as dystrophies, 6 as (non-dystrophic) myopathy and 4 regarded as references. From these sets of muscle biopsies, 135 completely measured muscle fibres were separated altogether, which were subjected to investigations using synchrotron radiation X-ray fluorescence (SR-XRF). Muscle fibres were analysed in terms of the composition of elements such as Br, Ca, Cl, Cr, Cu, Fe, K, Mn, P, S and Zn. The performed statistical tests indicate that all three groups (dystrophies—D; myopathies—M; references—R) show statistically significant differences in their elemental compositions, and the greatest impact, according to the multivariate discriminate analysis (MDA), comes from elements such as Ca, Cu, K, Cl and S. MDPI 2022-07-19 /pmc/articles/PMC9320641/ /pubmed/35887280 http://dx.doi.org/10.3390/ijms23147931 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kasprzyk, Paula
Wróbel, Paweł M.
Dudała, Joanna
Geraki, Kalotina
Szczerbowska-Boruchowska, Magdalena
Radwańska, Edyta
Krzyżewski, Roger M.
Adamek, Dariusz
Lankosz, Marek
Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)
title Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)
title_full Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)
title_fullStr Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)
title_full_unstemmed Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)
title_short Elemental Composition of Skeletal Muscle Fibres Studied with Synchrotron Radiation X-ray Fluorescence (SR-XRF)
title_sort elemental composition of skeletal muscle fibres studied with synchrotron radiation x-ray fluorescence (sr-xrf)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320641/
https://www.ncbi.nlm.nih.gov/pubmed/35887280
http://dx.doi.org/10.3390/ijms23147931
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