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Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida

In the current study, the anti-inflammatory and analgesic potential of Alnus nitida (leaves and fruits) was evaluated in the Sprague-Dawley rat. Traditionally, A. nitida was used for the treatment of inflammatory ailments. However, A. nitida leaves and fruits have not been yet reported regarding any...

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Autores principales: Sajid, Moniba, Khan, Muhammad Rashid, Ijaz, Muhammad Umar, Ismail, Hammad, Bhatti, Muhammad Zeeshan, Shah, Sayed Afzal, Ali, Saima, Tareen, Muhammad Usman, Alotaibi, Saqer S., Albogami, Sarah M., Batiha, Gaber El-Saber
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320741/
https://www.ncbi.nlm.nih.gov/pubmed/35889458
http://dx.doi.org/10.3390/molecules27144582
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author Sajid, Moniba
Khan, Muhammad Rashid
Ijaz, Muhammad Umar
Ismail, Hammad
Bhatti, Muhammad Zeeshan
Shah, Sayed Afzal
Ali, Saima
Tareen, Muhammad Usman
Alotaibi, Saqer S.
Albogami, Sarah M.
Batiha, Gaber El-Saber
author_facet Sajid, Moniba
Khan, Muhammad Rashid
Ijaz, Muhammad Umar
Ismail, Hammad
Bhatti, Muhammad Zeeshan
Shah, Sayed Afzal
Ali, Saima
Tareen, Muhammad Usman
Alotaibi, Saqer S.
Albogami, Sarah M.
Batiha, Gaber El-Saber
author_sort Sajid, Moniba
collection PubMed
description In the current study, the anti-inflammatory and analgesic potential of Alnus nitida (leaves and fruits) was evaluated in the Sprague-Dawley rat. Traditionally, A. nitida was used for the treatment of inflammatory ailments. However, A. nitida leaves and fruits have not been yet reported regarding any potential medicinal effects. Leaves/fruits of A. nitida were extracted with methanol and fractionated to attain n-hexane, chloroform, ethyl acetate and aqueous fractions. These extracts were then evaluated for in vivo analgesic and anti-inflammatory potential. For in vivo anti-inflammatory activity, carrageenan-induced paw edema assay, Freunds’ complete adjuvant-induced edema, xylene-induced ear edema and histamine-induced paw edema models were used in rats, which showed significant (p < 0.01) reduction (70–80%) in edema in comparison of inflammatory controls. On other hand, for the analgesic assessment, hot plate assay and acetic acid-induced writhing tests were used, which showed a significant (p < 0.01) rise in latency time (40–60%) as compared with pain-induced controls. These results were comparable with standard drugs in a concentration-dependent manner and no mortality or toxicity was observed during all experiments. Then, for the identification of chemical constituents gas chromatography–mass spectrometry (GC-MS) analysis was performed, which indicated the presence of neophytadiene, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, phytol and vitamin E, justifying the use of A. nitida to treat inflammatory disorders.
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spelling pubmed-93207412022-07-27 Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida Sajid, Moniba Khan, Muhammad Rashid Ijaz, Muhammad Umar Ismail, Hammad Bhatti, Muhammad Zeeshan Shah, Sayed Afzal Ali, Saima Tareen, Muhammad Usman Alotaibi, Saqer S. Albogami, Sarah M. Batiha, Gaber El-Saber Molecules Article In the current study, the anti-inflammatory and analgesic potential of Alnus nitida (leaves and fruits) was evaluated in the Sprague-Dawley rat. Traditionally, A. nitida was used for the treatment of inflammatory ailments. However, A. nitida leaves and fruits have not been yet reported regarding any potential medicinal effects. Leaves/fruits of A. nitida were extracted with methanol and fractionated to attain n-hexane, chloroform, ethyl acetate and aqueous fractions. These extracts were then evaluated for in vivo analgesic and anti-inflammatory potential. For in vivo anti-inflammatory activity, carrageenan-induced paw edema assay, Freunds’ complete adjuvant-induced edema, xylene-induced ear edema and histamine-induced paw edema models were used in rats, which showed significant (p < 0.01) reduction (70–80%) in edema in comparison of inflammatory controls. On other hand, for the analgesic assessment, hot plate assay and acetic acid-induced writhing tests were used, which showed a significant (p < 0.01) rise in latency time (40–60%) as compared with pain-induced controls. These results were comparable with standard drugs in a concentration-dependent manner and no mortality or toxicity was observed during all experiments. Then, for the identification of chemical constituents gas chromatography–mass spectrometry (GC-MS) analysis was performed, which indicated the presence of neophytadiene, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, phytol and vitamin E, justifying the use of A. nitida to treat inflammatory disorders. MDPI 2022-07-18 /pmc/articles/PMC9320741/ /pubmed/35889458 http://dx.doi.org/10.3390/molecules27144582 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sajid, Moniba
Khan, Muhammad Rashid
Ijaz, Muhammad Umar
Ismail, Hammad
Bhatti, Muhammad Zeeshan
Shah, Sayed Afzal
Ali, Saima
Tareen, Muhammad Usman
Alotaibi, Saqer S.
Albogami, Sarah M.
Batiha, Gaber El-Saber
Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
title Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
title_full Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
title_fullStr Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
title_full_unstemmed Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
title_short Evaluation of Phytochemistry and Pharmacological Properties of Alnus nitida
title_sort evaluation of phytochemistry and pharmacological properties of alnus nitida
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320741/
https://www.ncbi.nlm.nih.gov/pubmed/35889458
http://dx.doi.org/10.3390/molecules27144582
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