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Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy

Diabetic nephropathy (DN) remains the most common reason for end-stage renal disease and a leading cause of kidney replacement therapy. Multifactorial pathophysiological mechanisms underlie the development of DN. Among the signalling pathways involved, nuclear factor-κB (NF-κB) plays a key role in p...

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Autores principales: Abbad, Lilia, Prakoura, Niki, Michon, Arthur, Chalghoumi, Rym, Reichelt-Wurm, Simone, Banas, Miriam C., Chatziantoniou, Christos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320904/
https://www.ncbi.nlm.nih.gov/pubmed/35883655
http://dx.doi.org/10.3390/cells11142212
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author Abbad, Lilia
Prakoura, Niki
Michon, Arthur
Chalghoumi, Rym
Reichelt-Wurm, Simone
Banas, Miriam C.
Chatziantoniou, Christos
author_facet Abbad, Lilia
Prakoura, Niki
Michon, Arthur
Chalghoumi, Rym
Reichelt-Wurm, Simone
Banas, Miriam C.
Chatziantoniou, Christos
author_sort Abbad, Lilia
collection PubMed
description Diabetic nephropathy (DN) remains the most common reason for end-stage renal disease and a leading cause of kidney replacement therapy. Multifactorial pathophysiological mechanisms underlie the development of DN. Among the signalling pathways involved, nuclear factor-κB (NF-κB) plays a key role in pathogenesis triggering inflammation, oxidative stress and fibrosis. Recent evidence shows that periostin, a matricellular protein, is involved in the development of renal glomerular diseases through interaction with NF-κB signalling. The aim of the present study is to investigate the contribution of periostin and its interaction with NF-κB in DN development. To this end, we used the BTBR ob/ob mice model of diabetes type 2, and we applied transcriptomic analysis, immunostaining and methods quantifying protein and mRNA expressions. We found that increased periostin expression was correlated with decreased renal function, advanced stage renal damage and fibrosis, and NF-κB activation. Subsequently, we identified novel pathways and genes regulated by the NF-κB-periostin interaction which are involved in the mechanisms of progression of DN. Some of these genes, such as FGF1 and GDF15, have the potential to be new biomarkers and/or targets for the therapy of DN.
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spelling pubmed-93209042022-07-27 Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy Abbad, Lilia Prakoura, Niki Michon, Arthur Chalghoumi, Rym Reichelt-Wurm, Simone Banas, Miriam C. Chatziantoniou, Christos Cells Article Diabetic nephropathy (DN) remains the most common reason for end-stage renal disease and a leading cause of kidney replacement therapy. Multifactorial pathophysiological mechanisms underlie the development of DN. Among the signalling pathways involved, nuclear factor-κB (NF-κB) plays a key role in pathogenesis triggering inflammation, oxidative stress and fibrosis. Recent evidence shows that periostin, a matricellular protein, is involved in the development of renal glomerular diseases through interaction with NF-κB signalling. The aim of the present study is to investigate the contribution of periostin and its interaction with NF-κB in DN development. To this end, we used the BTBR ob/ob mice model of diabetes type 2, and we applied transcriptomic analysis, immunostaining and methods quantifying protein and mRNA expressions. We found that increased periostin expression was correlated with decreased renal function, advanced stage renal damage and fibrosis, and NF-κB activation. Subsequently, we identified novel pathways and genes regulated by the NF-κB-periostin interaction which are involved in the mechanisms of progression of DN. Some of these genes, such as FGF1 and GDF15, have the potential to be new biomarkers and/or targets for the therapy of DN. MDPI 2022-07-15 /pmc/articles/PMC9320904/ /pubmed/35883655 http://dx.doi.org/10.3390/cells11142212 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abbad, Lilia
Prakoura, Niki
Michon, Arthur
Chalghoumi, Rym
Reichelt-Wurm, Simone
Banas, Miriam C.
Chatziantoniou, Christos
Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy
title Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy
title_full Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy
title_fullStr Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy
title_full_unstemmed Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy
title_short Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy
title_sort role of periostin and nuclear factor-κb interplay in the development of diabetic nephropathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320904/
https://www.ncbi.nlm.nih.gov/pubmed/35883655
http://dx.doi.org/10.3390/cells11142212
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