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KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport

Photomorphogenic remodelling of seedling growth is a key developmental transition in the plant life cycle. The α/β‐hydrolase signalling protein KARRIKIN‐INSENSITIVE2 (KAI2), a close homologue of the strigolactone receptor DWARF14 (D14), is involved in this process, but it is unclear how the effects...

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Autores principales: Hamon‐Josse, Maxime, Villaécija‐Aguilar, José Antonio, Ljung, Karin, Leyser, Ottoline, Gutjahr, Caroline, Bennett, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320994/
https://www.ncbi.nlm.nih.gov/pubmed/35313031
http://dx.doi.org/10.1111/nph.18110
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author Hamon‐Josse, Maxime
Villaécija‐Aguilar, José Antonio
Ljung, Karin
Leyser, Ottoline
Gutjahr, Caroline
Bennett, Tom
author_facet Hamon‐Josse, Maxime
Villaécija‐Aguilar, José Antonio
Ljung, Karin
Leyser, Ottoline
Gutjahr, Caroline
Bennett, Tom
author_sort Hamon‐Josse, Maxime
collection PubMed
description Photomorphogenic remodelling of seedling growth is a key developmental transition in the plant life cycle. The α/β‐hydrolase signalling protein KARRIKIN‐INSENSITIVE2 (KAI2), a close homologue of the strigolactone receptor DWARF14 (D14), is involved in this process, but it is unclear how the effects of KAI2 on development are mediated. Here, using a combination of physiological, pharmacological, genetic and imaging approaches in Arabidopsis thaliana (Heynh.) we show that kai2 phenotypes arise because of a failure to downregulate auxin transport from the seedling shoot apex towards the root system, rather than a failure to respond to light per se. We demonstrate that KAI2 controls the light‐induced remodelling of the PIN‐mediated auxin transport system in seedlings, promoting a reduction in PIN7 abundance in older tissues, and an increase of PIN1/PIN2 abundance in the root meristem. We show that removing PIN3, PIN4 and PIN7 from kai2 mutants, or pharmacological inhibition of auxin transport and synthesis, is sufficient to suppress most kai2 seedling phenotypes. We conclude that KAI2 regulates seedling morphogenesis by its effects on the auxin transport system. We propose that KAI2 is not required for the light‐mediated changes in PIN gene expression but is required for the appropriate changes in PIN protein abundance within cells.
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spelling pubmed-93209942022-07-30 KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport Hamon‐Josse, Maxime Villaécija‐Aguilar, José Antonio Ljung, Karin Leyser, Ottoline Gutjahr, Caroline Bennett, Tom New Phytol Research Photomorphogenic remodelling of seedling growth is a key developmental transition in the plant life cycle. The α/β‐hydrolase signalling protein KARRIKIN‐INSENSITIVE2 (KAI2), a close homologue of the strigolactone receptor DWARF14 (D14), is involved in this process, but it is unclear how the effects of KAI2 on development are mediated. Here, using a combination of physiological, pharmacological, genetic and imaging approaches in Arabidopsis thaliana (Heynh.) we show that kai2 phenotypes arise because of a failure to downregulate auxin transport from the seedling shoot apex towards the root system, rather than a failure to respond to light per se. We demonstrate that KAI2 controls the light‐induced remodelling of the PIN‐mediated auxin transport system in seedlings, promoting a reduction in PIN7 abundance in older tissues, and an increase of PIN1/PIN2 abundance in the root meristem. We show that removing PIN3, PIN4 and PIN7 from kai2 mutants, or pharmacological inhibition of auxin transport and synthesis, is sufficient to suppress most kai2 seedling phenotypes. We conclude that KAI2 regulates seedling morphogenesis by its effects on the auxin transport system. We propose that KAI2 is not required for the light‐mediated changes in PIN gene expression but is required for the appropriate changes in PIN protein abundance within cells. John Wiley and Sons Inc. 2022-04-09 2022-07 /pmc/articles/PMC9320994/ /pubmed/35313031 http://dx.doi.org/10.1111/nph.18110 Text en © 2022 The Authors New Phytologist © 2022 New Phytologist Foundation https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hamon‐Josse, Maxime
Villaécija‐Aguilar, José Antonio
Ljung, Karin
Leyser, Ottoline
Gutjahr, Caroline
Bennett, Tom
KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport
title KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport
title_full KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport
title_fullStr KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport
title_full_unstemmed KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport
title_short KAI2 regulates seedling development by mediating light‐induced remodelling of auxin transport
title_sort kai2 regulates seedling development by mediating light‐induced remodelling of auxin transport
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320994/
https://www.ncbi.nlm.nih.gov/pubmed/35313031
http://dx.doi.org/10.1111/nph.18110
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