Cargando…

Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development

Multidrug antimicrobial resistance is a constantly growing health care issue associated with increased mortality and morbidity, and huge financial burden. Bacteria frequently form biofilm communities responsible for numerous persistent infections resistant to conventional antibiotics. Herein, novel...

Descripción completa

Detalles Bibliográficos
Autores principales: Ivanova, Aleksandra, Ivanova, Kristina, Fiandra, Luisa, Mantecca, Paride, Catelani, Tiziano, Natan, Michal, Banin, Ehud, Jacobi, Gila, Tzanov, Tzanko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321328/
https://www.ncbi.nlm.nih.gov/pubmed/35886883
http://dx.doi.org/10.3390/ijms23147527
_version_ 1784756018179211264
author Ivanova, Aleksandra
Ivanova, Kristina
Fiandra, Luisa
Mantecca, Paride
Catelani, Tiziano
Natan, Michal
Banin, Ehud
Jacobi, Gila
Tzanov, Tzanko
author_facet Ivanova, Aleksandra
Ivanova, Kristina
Fiandra, Luisa
Mantecca, Paride
Catelani, Tiziano
Natan, Michal
Banin, Ehud
Jacobi, Gila
Tzanov, Tzanko
author_sort Ivanova, Aleksandra
collection PubMed
description Multidrug antimicrobial resistance is a constantly growing health care issue associated with increased mortality and morbidity, and huge financial burden. Bacteria frequently form biofilm communities responsible for numerous persistent infections resistant to conventional antibiotics. Herein, novel nanoparticles (NPs) loaded with the natural bactericide farnesol (FSL NPs) are generated using high-intensity ultrasound. The nanoformulation of farnesol improved its antibacterial properties and demonstrated complete eradication of Staphylococcus aureus within less than 3 h, without inducing resistance development, and was able to 100% inhibit the establishment of a drug-resistant S. aureus biofilm. These antibiotic-free nano-antimicrobials also reduced the mature biofilm at a very low concentration of the active agent. In addition to the outstanding antibacterial properties, the engineered nano-entities demonstrated strong antiviral properties and inhibited the spike proteins of SARS-CoV-2 by up to 83%. The novel FSL NPs did not cause skin tissue irritation and did not induce the secretion of anti-inflammatory cytokines in a 3D skin tissue model. These results support the potential of these bio-based nano-actives to replace the existing antibiotics and they may be used for the development of topical pharmaceutic products for controlling microbial skin infections, without inducing resistance development.
format Online
Article
Text
id pubmed-9321328
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93213282022-07-27 Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development Ivanova, Aleksandra Ivanova, Kristina Fiandra, Luisa Mantecca, Paride Catelani, Tiziano Natan, Michal Banin, Ehud Jacobi, Gila Tzanov, Tzanko Int J Mol Sci Article Multidrug antimicrobial resistance is a constantly growing health care issue associated with increased mortality and morbidity, and huge financial burden. Bacteria frequently form biofilm communities responsible for numerous persistent infections resistant to conventional antibiotics. Herein, novel nanoparticles (NPs) loaded with the natural bactericide farnesol (FSL NPs) are generated using high-intensity ultrasound. The nanoformulation of farnesol improved its antibacterial properties and demonstrated complete eradication of Staphylococcus aureus within less than 3 h, without inducing resistance development, and was able to 100% inhibit the establishment of a drug-resistant S. aureus biofilm. These antibiotic-free nano-antimicrobials also reduced the mature biofilm at a very low concentration of the active agent. In addition to the outstanding antibacterial properties, the engineered nano-entities demonstrated strong antiviral properties and inhibited the spike proteins of SARS-CoV-2 by up to 83%. The novel FSL NPs did not cause skin tissue irritation and did not induce the secretion of anti-inflammatory cytokines in a 3D skin tissue model. These results support the potential of these bio-based nano-actives to replace the existing antibiotics and they may be used for the development of topical pharmaceutic products for controlling microbial skin infections, without inducing resistance development. MDPI 2022-07-07 /pmc/articles/PMC9321328/ /pubmed/35886883 http://dx.doi.org/10.3390/ijms23147527 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ivanova, Aleksandra
Ivanova, Kristina
Fiandra, Luisa
Mantecca, Paride
Catelani, Tiziano
Natan, Michal
Banin, Ehud
Jacobi, Gila
Tzanov, Tzanko
Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
title Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
title_full Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
title_fullStr Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
title_full_unstemmed Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
title_short Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
title_sort antibacterial, antibiofilm, and antiviral farnesol-containing nanoparticles prevent staphylococcus aureus from drug resistance development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321328/
https://www.ncbi.nlm.nih.gov/pubmed/35886883
http://dx.doi.org/10.3390/ijms23147527
work_keys_str_mv AT ivanovaaleksandra antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT ivanovakristina antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT fiandraluisa antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT manteccaparide antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT catelanitiziano antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT natanmichal antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT baninehud antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT jacobigila antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment
AT tzanovtzanko antibacterialantibiofilmandantiviralfarnesolcontainingnanoparticlespreventstaphylococcusaureusfromdrugresistancedevelopment